Endothelial juxtaposition of distinct adult stem cells activates angiogenesis signaling molecules in endothelial cells

Mohammadi, Elham; Nassiri, Seyed Mahdi; Rahbarghazi‬, Reza; Siavashi, Vahid; Araghi, Atefeh

doi:10.1007/s00441-015-2228-2

Cited by 38 publications

(33 citation statements)

References 46 publications

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“…It is a normal and vital process in growth and development, as well as in wound healing [2]. In contrast, pathological angiogenesis is observed in a wide range of diseases, including tumor growth, lipid metabolism disorders and chronic inflammation, as a result of excessive pro-angiogenic signals and a lack of sufficient factors to mediate vessel maturation [3].…”

Section: Introductionmentioning

confidence: 99%

DEPTOR Deficiency-Mediated mTORc1 Hyperactivation in Vascular Endothelial Cells Promotes Angiogenesis

Ding

Shan

Nai

et al. 2018

Cell Physiol Biochem

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Background/Aims: The mechanistic target of rapamycin (mTOR) signaling pathway is essential for angiogenesis and embryonic development. DEP domain-containing mTOR-interacting protein (DEPTOR) is an mTOR binding protein that functions to inhibit the mTOR pathway In vitro experiments suggest that DEPTOR is crucial for vascular endothelial cell (EC) activation and angiogenic responses. However, knowledge of the effects of DEPTOR on angiogenesis in vivo is limited. This study aimed to determine the role of DEPTOR in tissue angiogenesis and to elucidate the molecular mechanisms. Methods: Cre/loxP conditional gene knockout strategy was used to delete the Deptor gene in mouse vascular ECs. The expression or distribution of cluster of differentiation 31 (CD31), vascular endothelial growth factor (VEGF) and hypoxia inducible factor-1 alpha (HIF-1α) were detected by immunohistochemical staining or western blot. Tube formation assay was used to measure angiogenesis in vitro. Results: Deptor knockdown led to increased expression of CD31, VEGF and HIF-1α in heart, liver, kidney and aorta. After treatment with rapamycin, their expression was significantly down regulated. In vitro, human umbilical vein endothelial cells (HUVECs) were transfected with DEPTOR-specific small interfering RNA (siRNA), which resulted in a significant increase in endothelial tube formation and migration rates. In contrast, DEPTOR overexpression markedly reduced the expression of CD31, VEGF and HIF-1α. Conclusions: Our findings demonstrated that deletion of the Deptor gene in vascular ECs resulted in upregulated expression of CD31 and HIF-1α, and further stimulated the expression of VEGF which promoted angiogenesis, indicating that disruption of normal angiogenic pathways may occur through hyperactivation of the mTORC1/HIF-1α/VEGF signaling pathway.

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Section: Introductionmentioning

confidence: 99%

DEPTOR Deficiency-Mediated mTORc1 Hyperactivation in Vascular Endothelial Cells Promotes Angiogenesis

Ding

Shan

Nai

et al. 2018

Cell Physiol Biochem

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“…1.44p (NIH, Sacaton, AZ) in order to measure the tube length as well as to score cell colonies for tubulogenesis. Tubulogenesis was scored using a colony scoring system according to our previous publications . Briefly, 25 colonies were examined in each well and a 0 to 4 score was given to each colony as follows: 0, aggregate colony with no sprouting; (1) colony sprouting without arborization; (2) with arborization; (3) with anastomosis; and (4) development of a complex tubular network.…”

Section: Methodsmentioning

confidence: 99%

“…Western blot analysis was performed as previously described . In brief, cell lysates were mixed with equal volume of 2× Laemmli sample buffer.…”

Section: Methodsmentioning

confidence: 99%

Increased circulation mobilization of endothelial progenitor cells in preterm infants with retinopathy of prematurity

Babaei

Alibabrdel

Asadian

et al. 2018

J of Cellular Biochemistry

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Retinopathy of prematurity (ROP) is a result of increased pathological neoangiogenesis of the retina in preterm infants. Cells responsible for the pathogenesis of ROP are unclear, but some evidence indicates that bone marrow derived cells are involved in this disorder. Endothelial progenitor cells (EPCs), play a role in angiogenesis in response to tissue ischemia or endothelial damage. In this study, the number of cEPCs in preterm infants with ROP was determined to identify whether the circulation mobilization of EPCs is associated with ROP. We evaluated 99 participants in this study: 22 preterm infants with ROP, 35 preterm infants without ROP, and 42 full-term infants. The release of EPCs in the circulation was first quantified. Thereafter, cEPCs were harvested and cultivated, then the biological features of these cells including migratory, proliferative, and tubulogenic activities were analyzed. The mRNA levels of some proangiogenic factors were also measured in preterm infants. Our results showed greater numbers of cEPCs in infants with ROP, which was associated with increased serum concentrations of angiogenic factors and with augmented proliferative, migratory, and tubulogenic activity of these cells. Western blotting showed increased protein levels of VEGF and HIF-α in cEPCs harvested from ROP infants. This study showed that ROP in preterm infants is associated with increased mobilization of EPCs into the circulation. Therefore, increased cEPCs along with elevated levels of angiogenic factors and tubulogenesis suggest that these cells may play a role in the development and progression of ROP.

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“…1.44p (NIH, USA) to quantify the tube length. Tubulogenesis was scored using a colony scoring system according to our previous publications (Mohammadi et al 2015). Briefly, 25 colonies were examined in each well and a 0-4 score was assigned to each colony as follows: 0, aggregate colony with no sprouting; 1, colony sprouting without arborization; 2, with arborization; 3, with anastomosis; and 4, development of a complex tubular network.…”

Section: Effects Of Ang-1 and Ang-2 On Matrigel Tubulogenesis Of Cultmentioning

confidence: 99%

“…Densitometry of proteins was performed using the ImageJ Version 1.44p software (NIH, USA). The percentage area under the curve of each band was divided by the corresponding percentage area under the curve of the actin band, and the calculated values were compared statistically between the C and T groups (Mohammadi et al 2015).…”

Section: Western Blot Analysismentioning

confidence: 99%

Angiogenic activity of endothelial progenitor cells through angiopoietin-1 and angiopoietin-2

Siavashi

Sariri

Nassiri

et al. 2016

Animal Cells and Systems

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Angiogenesis is a regulated process involving the proliferation, migration, and remodeling of different cell types particularly mature endothelial cells and recently discovered progenitor cells, named as endothelial progenitor cells (EPCs). Up to now, many attempts have been made to understand the dynamic balance of pro-and anti-angiogenic factors on EPCs on different milieu. It has been accepted that Ang-1, -2 and Tie-1, -2 signaling play a key role on angiogenesis pathways in endothelial lineage cells. In the current experiment, the angiogenic/angiomodulatory potency of Ang-1 and -2 was investigated on isolated EPCs. Freshly isolated EPCs were exposed to different concentrations of Ang-1 and -2 (25 and 50 ng/ml) over a course of 7 and 14 days. Corroborating to our results, a superior effect of Ang-1 on angiogenic properties, including an increased concentration of vascular endothelial growth factor, in vitro tubulogenesis, EPC migratory, Tie-2 expression and clonogenicity, was determined. A large amount of positive mature endothelium markers was achieved in EPCs being-exposed to Ang-1 peptide. Nonetheless, the number of CD133 positive cells increased in the presence of Ang-2. Collectively, we conclude that Ang-1 potentially induces functional and mature vascular-like behavior in EPCs more than Ang-2.ARTICLE HISTORY

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Endothelial juxtaposition of distinct adult stem cells activates angiogenesis signaling molecules in endothelial cells

Cited by 38 publications

References 46 publications

DEPTOR Deficiency-Mediated mTORc1 Hyperactivation in Vascular Endothelial Cells Promotes Angiogenesis

DEPTOR Deficiency-Mediated mTORc1 Hyperactivation in Vascular Endothelial Cells Promotes Angiogenesis

Increased circulation mobilization of endothelial progenitor cells in preterm infants with retinopathy of prematurity

Angiogenic activity of endothelial progenitor cells through angiopoietin-1 and angiopoietin-2

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