Endothelial damage and vascular calcification in patients with chronic kidney disease

Soriano, Sagrario; Carmona, Andrés; Triviño, Francisco; Rodriguez, Mariano; Marina, Álvarez-Benito,; Martín‐Malo, Alejandro; Alvarez-Lara, Maria-Antonia; Ramı́rez, Rafael; Aljama, Pedro; Carracedo, Julia

doi:10.1152/ajprenal.00114.2014

Cited by 60 publications

(69 citation statements)

References 43 publications

(51 reference statements)

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“…Activated endothelial cells in uremia are capable of producing microparticles that induce OC expression in endothelial progenitor cells, VSMCs and fibroblast. The imbalance in the process of endothelial damage and repair, therefore, may contribute to VC via increasing the number of OC-positive endothelial progenitor cells [34]. …”

Section: Discussionmentioning

confidence: 99%

The Impact of Osteocalcin, Osteoprotegerin and Osteopontin on Arterial Stiffness in Chronic Renal Failure Patients on Hemodialysis

Csiky

Sági

Peti³

et al. 2017

Kidney Blood Press Res

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Background/Aims: This cross-sectional study was designed to assess the relationship between vascular stiffness (VS) and bone-related proteins involved in the development of arteriosclerosis in patients on regular hemodialysis (HD). Methods: 68 consecutive patients in stable clinical condition who received regular HD in the FMC Dialysis Center, Pécs were included. VS parameters (carotid-femoral pulse wave velocity – PWV, aortic augmentation index - AIx) were determined by applanation tonometry (SphygmoCor, AtCor Medical, Sidney) and the routine latoratory test were completed with measurements of osteocalcin (OC), osteopontin (OP) and osteoprotegerin (OPG) by using commercially available ELISA kits. 35 heathcare workers served as controls. Results: In patients on regular HD PWV markedly increased and there was several-fold elevation in the interrelated bone-specific proteins (OC, OP, OPG). PWV was found to be independently associated only with OC (β:-0.25, p<0.029) and age (r=0.411,p<0.000), but risk factors for arterial calcification had significant impact on OC (systolic blood pressure, hsCRP, BMI), OPG (age, BMI) and OP (LDL-cholesterol). Conclusion: Except for OC, our results failed to document direct association of vascular lesion with OP and OPG, therefore their high circulating levels may be an epiphenomenon or they may have counter-regulatory role to attenuate the uremic calcification process.

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Section: Discussionmentioning

confidence: 99%

The Impact of Osteocalcin, Osteoprotegerin and Osteopontin on Arterial Stiffness in Chronic Renal Failure Patients on Hemodialysis

Csiky

Sági

Peti³

et al. 2017

Kidney Blood Press Res

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“…diabetes, kidney disease, atherosclerosis and coronary artery diseases) endothelial damages are also associated with vascular calcification [22]. It has been demonstrated that procalcific polarization of circulating progenitors may contribute to vascular mineralization in patients exhibiting a calcifying potential in vitro [9,23].…”

Section: Discussionmentioning

confidence: 99%

Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction

et al. 2016

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IntroductionAlthough rare, circulating endothelial and progenitor cells could be considered as markers of endothelial damage and repair potential, possibly predicting the severity of cardiovascular manifestations. A number of studies highlighted the role of these cells in age-related diseases, including those characterized by ectopic calcification. Nevertheless, their use in clinical practice is still controversial, mainly due to difficulties in finding reproducible and accurate methods for their determination.MethodsCirculating mature cells (CMC, CD45-, CD34+, CD133-) and circulating progenitor cells (CPC, CD45dim, CD34bright, CD133+) were investigated by polychromatic high-speed flow cytometry to detect the expression of endothelial (CD309+) or osteogenic (BAP+) differentiation markers in healthy subjects and in patients affected by peripheral vascular manifestations associated with ectopic calcification.ResultsThis study shows that: 1) polychromatic flow cytometry represents a valuable tool to accurately identify rare cells; 2) the balance of CD309+ on CMC/CD309+ on CPC is altered in patients affected by peripheral vascular manifestations, suggesting the occurrence of vascular damage and low repair potential; 3) the increase of circulating cells exhibiting a shift towards an osteoblast-like phenotype (BAP+) is observed in the presence of ectopic calcification.ConclusionDifferences between healthy subjects and patients with ectopic calcification indicate that this approach may be useful to better evaluate endothelial dysfunction in a clinical context.

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“…Circulating MPs have been the most studied EVs in humans with CKD and ESRD, conditions in which MPs have been associated with endothelial dysfunction, 67 cardiovascular mortality, 68,69 increased risk for calcification, 70,71 anemia, 72,73 and disease-specific alteration in coagulation. 74 In a study of a heterogeneous group of patients with chronic renal failure and those on peritoneal dialysis and hemodialysis, all patients compared with healthy controls had higher levels of endothelial-derived microparticles (EMPs) and MPs of leukocyte origin.…”

Section: Ckd/uremia/esrdmentioning

confidence: 99%

Extracellular Vesicles in Renal Diseases

Erdbrügger

2016

Journal of the American Society of Nephrology

165

175

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Extracellular vesicles from the urine and circulation have gained significant interest as potential diagnostic biomarkers in renal diseases. Urinary extracellular vesicles contain proteins from all sections of the nephron, whereas most studied circulating extracellular vesicles are derived from platelets, immune cells, and the endothelium. In addition to their diagnostic role as markers of kidney and vascular damage, extracellular vesicles may have functional significance in renal health and disease by facilitating communication between cells and protecting against kidney injury and bacterial infection in the urinary tract. However, the current understanding of extracellular vesicles has derived mostly from studies with very small numbers of patients or in vitro data. Moreover, accurate assessment of these vesicles remains a challenge, in part because of a lack of consensus in the methodologies to measure extracellular vesicles and the inability of most techniques to capture the entire size range of these vesicles. However, newer techniques and standardized protocols to improve the detection of extracellular vesicles are in development. A clearer understanding of the composition and biology of extracellular vesicles will provide insights into their pathophysiologic, diagnostic, and therapeutic roles.

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Endothelial damage and vascular calcification in patients with chronic kidney disease

Abstract: Carracedo J. Endothelial damage and vascular calcification in patients with chronic kidney disease. Am J Physiol

Cited by 60 publications

References 43 publications

The Impact of Osteocalcin, Osteoprotegerin and Osteopontin on Arterial Stiffness in Chronic Renal Failure Patients on Hemodialysis

The Impact of Osteocalcin, Osteoprotegerin and Osteopontin on Arterial Stiffness in Chronic Renal Failure Patients on Hemodialysis

Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction

Extracellular Vesicles in Renal Diseases

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