Endothelial colony-forming cells and mesenchymal stem cells from ECMO circuits of term infants

Abstract

Objective: Endothelial progenitor cells (EPCs) have been examined in numerous adult diseases and have been suggested as a cellular-based therapy. However, there are no reports describing EPCs being isolated from newborn peripheral blood.Study Design: Endothelial colony-forming cells (ECFCs), a subtype of EPCs, were isolated from blood collected from 12 neonatal extracorporeal membrane oxygenation (ECMO) circuits.Result: ECFCs were isolated in all samples. We unexpectedly isolated a distinctly different colony of mesenchymal stem cells (MSCs) in seven samples. Both cell types expressed the expected endothelial or mesenchymal cell surface antigens. Conclusion:To our knowledge, this is the first report of ECFCs and MSCs isolated from peripheral blood of critically ill term newborns. Both cells types may be mobilized in response to critical illness or to the ECMO circuit. Further studies evaluating the role of stem cells in various newborn conditions are warranted.

Accumulation of Multipotent Progenitor Cells on Polymethylpentene Membranes During Extracorporeal Membrane Oxygenation

“…Similar differentiation was also described by Hösli et al. . However, there was no correlation between the type and number of colonies isolated and any clinical outcome (mortality, length of mechanical ventilation, length of oxygen therapy).…”

“…The monocytoid cells that weakly expressed all used cell markers were not described in other studies . They were suppressed by usage of differentiation media to select for specific progenitor cells or excluded by cloning single colonies . It can't be excluded that that the CD45+/CD31+ cells were capable to differentiate into endothelial‐like cells (CD45−/CD31+) or mesenchymal‐like cells (CD45−/CD31−).…”

“…However, the fluorescence intensity of both markers gradually decreased and aggravated demarcation to the CD45−/CD31− cell population. The monocytoid cells that weakly expressed all used cell markers were not described in other studies . They were suppressed by usage of differentiation media to select for specific progenitor cells or excluded by cloning single colonies .…”

“…There is even evidence that survival rate increased with higher levels of EPCs (4). In addition, treatment with ECMO might also cause mobilization of progenitor cells (6,7). However, there was no correlation between ECMO treatment, increase in progenitor cell concentration, and improvement of disease.…”

Expression and function of toll-like receptors in human circulating endothelial colony forming cells

“…Conversely, the bone marrow (BM) of adult healthy individuals and the umbilical cord blood (UCB) of preterm and full-term neonates contain higher numbers of ECFCs, with UCB-ECFCs displaying higher proliferative and clonogenic potential compared to adult BM-and PB-ECFC [1,[9][10][11][12]. Circulating PB-and UCB-ECFCs may vary in frequency in certain pathological conditions [13][14][15][16].…”