Endothelial colony-forming cell conditioned media promote angiogenesis in vitro and prevent pulmonary hypertension in experimental bronchopulmonary dysplasia

Baker, Christopher D.; Seedorf, Gregory J.; Wisniewski, B; Black, Claudine P.; Ryan, Sharon; Balasubramaniam, Vivek; Abman, Steven H.

doi:10.1152/ajplung.00400.2012

Cited by 87 publications

(71 citation statements)

References 42 publications

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“…Here, cells are injected directly at the site of vascular ligation to ascertain their angiogenic and neovasculogenic capacity [40,41]. Assessing candidate EPC populations by quantification has also been attempted in pathologies such as diabetes, preeclampsia, and bronchopulmonary dysplasia [42][43][44][45][46]. Since their discovery in 2004, there has been a growing body of evidence to suggest that ECFCs play a role in regenerating vascular structures and can form de novo vessels when injected into an ischemic site [15,[47][48][49].…”

Section: Potency Of Epcsmentioning

confidence: 99%

Concise Review: Functional Definition of Endothelial Progenitor Cells: A Molecular Perspective

Patel

Donovan

Khosrotehrani

2016

Stem Cells Translational Medicine

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Notch signaling driving the quiescence of progenitors has been shown to be central to progenitor self‐renewal. This new molecular definition has tremendous translational consequences because progenitors have been shown to display greater vasculogenic potential. This molecular definition of endothelial progenitor cell (EPC) self‐renewal allows assessment of the quality of presumed EPC preparations.

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Section: Potency Of Epcsmentioning

confidence: 99%

Concise Review: Functional Definition of Endothelial Progenitor Cells: A Molecular Perspective

Patel

Donovan

Khosrotehrani

2016

Stem Cells Translational Medicine

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“…[38] ECFC Decreased in preterm human CB exposed to hyperoxia in vitro [6] ECFC Decreased in preterm human CB exposed to hyperoxia in vitro. Impaired expression of VEGFR-2+ and eNOS.…”

Section: Cell Characterizationmentioning

confidence: 97%

“…Studies using both term and preterm ECFC conditioned medium (ECFC-CM) from cord blood have shown that the conditioned medium contained soluble factors that promote the growth of fetal sheep pulmonary artery endothelial cells, fetal alveolar type 2 cells and angiogenesis in vitro [38].…”

Section: The Role Of Bone Marrow Derived Cell Types and Soluble Factomentioning

confidence: 99%

“…When bleomycin treated newborn rats (showing impaired vascular and alveolar growth and secondary pulmonary hypertension, but no pulmonary fibrosis) were used as an experimental model of BPD, ECFC-CM reduced right ventricular hypertrophy (RVH), but did not increase alveolar septation [38]. It has long been debated whether the EPC affect vascular repair by engraftment in the injured area.…”

Section: The Role Of Bone Marrow Derived Cell Types and Soluble Factomentioning

confidence: 99%

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Circulating Endothelial Progenitors and Bone Marrow Derived Cells as Biomarkers for Risk of Bronchopulmonary Dysplasia

Kirzner¹

2014

J Bone Marrow Res

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Bronchopulmonary dysplasia (BPD), also known as neonatal chronic lung disease, is a multifactorial disease and its pathogenesis starts even before birth. Animal models of BPD and the study of infants with BPD suggest that impaired lung vascular development leads to the failure of alveolar development and strategies that promote vascular development result in improved alveolarization of the lung. Since 1997 when Asahara identified endothelial cell progenitors (EPCs) as blood cells having the ability to contribute to postnatal vasculogenesis, several studies have attempted to elucidate the role of EPCs in neonatal lung development and lung injury and repair. This review outlines the progress in defining early EPCs (believed to be of hematopoietic origin) and late EPCs or true EPCs (believed to be of endothelial origin) through the use of cell culture assays and flow cytometric characterization. Both animal and human studies have attempted to correlate the frequency of these specific populations with susceptibility to BPD. Animal studies use hyperoxia or endotoxininduced lung injury as a model of BPD. Human studies use frequencies of specific cell populations as a prognostic index of BPD. Conflicting outcomes are likely the result of a lack of consistent definitions. Recently, there is increasing evidence that blood and bone marrow-derived stem cells exert a beneficial effect in models of chronic lung injury, not so much by engraftment and differentiation, but by a paracrine effect on the existing lung progenitor cells.

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“…[19][20][21] Demonstrations of the critical role of the "secretome" in mediating proangiogenic effects of EPCs and other progenitor cells, independent of cell engraftment, further illustrate the close interplay between vasculogenic and angiogenic mechanisms. [22][23][24] During formation of secondary septae in the alveolar stage of lung development, the double capillary network in the immature gas-exchange region fuses to form a single capillary layer. 25,26 Between birth and adulthood, the alveolar and capillary surface areas expand nearly 20-fold and the capillary volume by 35-fold, primarily by sprouting angiogenesis from preexisting vessels and through energy-efficient intussusceptive growth.…”

Section: Impaired Lung Vascular Development In Pediatric Pah General mentioning

confidence: 99%

The Robyn Barst Memorial Lecture: Differences between the Fetal, Newborn, and Adult Pulmonary Circulations: Relevance for Age‐Specific Therapies (2013 Grover Conference Series)

et al. 2014

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Pulmonary arterial hypertension (PAH) contributes to poor outcomes in diverse diseases in newborns, infants, and children. Many aspects of pediatric PAH parallel the pathophysiology and disease courses observed in adult patients; however, critical maturational differences exist that contribute to distinct outcomes and therapeutic responses in children. In comparison with adult PAH, disruption of lung vascular growth and development, or angiogenesis, plays an especially prominent role in the pathobiology of pediatric PAH. In children, abnormalities of lung vascular development have consequences well beyond the adverse hemodynamic effects of PAH alone. The developing endothelium also plays critical roles in development of the distal airspace, establishing lung surface area for gas exchange and maintenance of lung structure throughout postnatal life through angiocrine signaling. Impaired functional and structural adaptations of the pulmonary circulation during the transition from fetal to postnatal life contribute significantly to poor outcomes in such disorders as persistent pulmonary hypertension of the newborn, congenital diaphragmatic hernia, bronchopulmonary dysplasia, Down syndrome, and forms of congenital heart disease. In addition, several studies support the hypothesis that early perinatal events that alter lung vascular growth or function may set the stage for increased susceptibility to PAH in adult patients ("fetal programming"). Thus, insights into basic mechanisms underlying unique features of the developing pulmonary circulation, especially as related to preservation of endothelial survival and function, may provide unique therapeutic windows and distinct strategies to improve short-and long-term outcomes of children with PAH.Keywords: pulmonary vascular development, angiogenesis, alveolarization, persistent pulmonary hypertension of the newborn, congenital diaphragmatic hernia, bronchopulmonary dysplasia, Down syndrome, pediatric pulmonary hypertension. Pulmonary arterial hypertension (PAH) is a devastating and progressive disease that is characterized by abnormalities of vascular tone and reactivity, vessel wall structure, growth, and thrombosis. 1 Mechanisms contributing to PAH and its progression are complex and remain incompletely understood. Insights into vascular biology over the past few decades have led to remarkable improvements in the clinical course, outcomes, and survival in adults with PAH, yet relatively few studies have been performed in children. As in adults, PAH contributes to poor outcomes in diverse cardiac, pulmonary, and systemic diseases in newborns, infants, and children (Table 1). 2 There is a clear need to define the natural history of pediatric PAH in diverse settings; to develop new strategies to identify at-risk patients early in their course; and to establish novel approaches to better diagnose, evaluate, and treat children with PAH. 3,4 Despite many similarities between pediatric and adult PAH, critical differences exist that currently limit our clinical appr...

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Endothelial colony-forming cell conditioned media promote angiogenesis in vitro and prevent pulmonary hypertension in experimental bronchopulmonary dysplasia

Abstract: Baker CD, Seedorf GJ, Wisniewski BL, Black CP, Ryan SL, Balasubramaniam V, Abman SH. Endothelial colony-forming cell conditioned media promote angiogenesis in vitro and prevent pulmonary hypertension in experimental bronchopulmonary dysplasia.

Cited by 87 publications

References 42 publications

Concise Review: Functional Definition of Endothelial Progenitor Cells: A Molecular Perspective

Concise Review: Functional Definition of Endothelial Progenitor Cells: A Molecular Perspective

Circulating Endothelial Progenitors and Bone Marrow Derived Cells as Biomarkers for Risk of Bronchopulmonary Dysplasia

The Robyn Barst Memorial Lecture: Differences between the Fetal, Newborn, and Adult Pulmonary Circulations: Relevance for Age‐Specific Therapies (2013 Grover Conference Series)

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