Endothelial Cell Response to Fusobacterium nucleatum

Abstract

c Vascular response is an essential aspect of an effective immune response to periodontal disease pathogens, as new blood vessel formation contributes to wound healing and inflammation. Gaining a greater understanding of the factors that affect vascular response may then contribute to future breakthroughs in dental medicine. In this study, we have characterized the endothelial cell response to the common bacterium Fusobacterium nucleatum, an important bridging species that facilitates the activity of late colonizers of the dental biofilm. Endothelial cells were infected with Fusobacterium nucleatum (strain 25586) for periods of 4, 12, 24, or 48 h. Cell proliferation and tube formation were analyzed, and expression of adhesion molecules (CD31 and CD34) and vascular endothelial growth factor (VEGF) receptors 1 and 2 was measured by fluorescenceactivated cell sorter (FACS) analysis. Data indicate that F. nucleatum impaired endothelial cell proliferation and tube formation. The findings suggest that the modified endothelial cell response acts as a mechanism promoting the pathogenic progression of periodontal diseases and may potentially suggest the involvement of periodontopathogens in systemic diseases associated with periodontal inflammation.

Persistent Exposure to Fusobacterium nucleatum Triggers Chemokine/Cytokine Release and Inhibits the Proliferation and Osteogenic Differentiation Capabilities of Human Gingiva-Derived Mesenchymal Stem Cells

“…In this study, we demonstrate that F. nucleatum inhibits the proliferation of GMSCs. It has been reported that F. nucleatum can impair endothelial cell and mononuclear cell proliferation, which is consistent with our finding in GMSCs (Haake and Lindemann, 1997;Mendes et al, 2016). Viable and formaldehyde-treated, but not sonicated or heat-treated F. nucleatum bacteria, can cause severe aggregation and apoptosis of peripheral blood mononuclear cells (PBMCs) (Huynh et al, 2011).…”

Fusobacterium nucleatum and colorectal cancer: A mechanistic overview

“…Under hypoxia condition, hypoxia‐inducible factor‐1 (HIF‐1) increases the expression of angiogenesis growth factors, including vascular endothelial growth factor (VEGF) and its receptors (VEGFRs; Hashemi Goradel et al, ). Consistent with this proposal, Mendes et al () reported that infection of epithelial cells with F. nucleatum increases angiogenesis via upregulating VEGF and VEGFR1 and 2. Another effect of hypoxia and HIF‐1 in tumor microenvironment is related to the promotion of myeloid‐derived suppressor cells (MDSCs), which act as immunosuppressive cells in cancer (Chiu et al, ).…”

Green tea prevents vascular disturbs and attenuates periodontal breakdown in long‐term hyperglycaemia in T1D rats

“…Vascular response is an essential aspect of an effective immune response to periopathogens (Mendes et al., ). In our study, the higher periodontal and dental tissue loss observed in T1D‐W rats was associated with vascular alteration in PDL.…”