Endothelial Cell Membrane Sensitivity to Shear Stress is Lipid Domain Dependent

Tabouillot, Tristan; Muddana, Hari S.; Butler, Peter J.

doi:10.1007/s12195-010-0136-9

Cited by 41 publications

(46 citation statements)

References 74 publications

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“…GPCRs, such as rhodopsin have also been shown to be very sensitive to comparable changes in membrane thickness and lipid composition (which affects various bilayer parameters, including fluidity) [9,94,95]. Notably it has also been reported that mechanical perturbation of the cell membrane leads to membrane fluidity changes of similar magnitude as determined in this study [22,23,96,97]. Although changes in membrane fluidity may not directly affect conformational equilibrium between active and inactive states of the membrane protein since populations of conformational states are primarily determined by the free energy differences, the fluidity could have an effect on downstream signaling.…”

Section: Discussionsupporting

confidence: 68%

Effect of membrane tension on the physical properties of DOPC lipid bilayer membrane

Reddy

Warshaviak

Chachisvilis

2012

Biochimica et Biophysica Acta (BBA) - Biomembranes

106

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Molecular dynamics simulations of a dioleoylphosphocholine (DOPC) lipid bilayer were performed to explore its mechanosensitivity. Variations in the bilayer properties, such as area per lipid, volume, thickness, hydration depth (HD), hydration thickness (HT), lateral diffusion coefficient, and changes in lipid structural order were computed in the membrane tension range 0 to 15 dyn/cm. We determined that an increase in membrane tension results in a decrease in the bilayer thickness and HD of ∼5% and ∼5.7% respectively, whereas area per lipid, volume, and HT/HD increased by 6.8%, 2.4%, and 5% respectively. The changes in lipid conformation and orientation were characterized using orientational (S2) and deuterium (SCD) order parameters. Upon increase of membrane tension both order parameters indicated an increase in lipid disorder by 10– 20%, mostly in the tail end region of the hydrophobic chains. The effect of membrane tension on lipid lateral diffusion in the DOPC bilayer was analyzed on three different time scales corresponding to inertial motion, anomalous diffusion and normal diffusion. The results showed that lateral diffusion of lipid molecules is anomalous in nature due to the non-exponential distribution of waiting times. The anomalous and normal diffusion coefficients increased by 20% and 52% when the membrane tension changed from 0 to 15 dyn/cm, respectively. In conclusion, our studies showed that membrane tension causes relatively significant changes in the area per lipid, volume, polarity, membrane thickness, and fluidity of the membrane suggesting multiple mechanisms by which mechanical perturbation of the membrane could trigger mechanosensitive response in cells.

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Section: Discussionsupporting

confidence: 68%

Effect of membrane tension on the physical properties of DOPC lipid bilayer membrane

Reddy

Warshaviak

Chachisvilis

2012

Biochimica et Biophysica Acta (BBA) - Biomembranes

106

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“…tension) of the membrane. [23][24] In time-resolved fluorescence measurements, we observe a clear difference in the fluorescence lifetime of the DiI between the living cell and the GPMV. Suggesting the cell membrane may be maintained in a different region of the phase diagram and may even actively avoid a temperature-driven miscibility phase transition.…”

Section: Introductionmentioning

confidence: 89%

“…8 The fluorescence lifetime of DiI is linearly correlated with the local viscosity of the dye's surrounding environment, so DiI fluorescence lifetime can be used as membrane tension reporter. 23 We collected data without distinguishing the different phases of phase separated GPMV to correctly mimic the data collection from the cell membrane with hypothetical nano-scale phase separation. …”

Section: Lifetime Measurementmentioning

confidence: 99%

Live Cell Plasma Membranes Do Not Exhibit a Miscibility Phase Transition over a Wide Range of Temperatures

et al. 2015

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Lipid:cholesterol mixtures derived from cell membranes, as well as their synthetic reconstitutions, exhibit well defined miscibility phase transitions and critical phenomena near physiological temperatures. This suggests that lipid:cholesterol-mediated phase separation plays a role in the organization of live cell membranes. However, macroscopic lipid phase separation is not generally observed in cell membranes and the degree to which properties of isolated lipid mixtures are preserved in the cell membrane remain unknown. A fundamental property of phase transitions is that the variation of tagged particle diffusion with temperature exhibits an abrupt change as the system passes through the transition, even when the two phases are distributed in a nanometer-scale emulsion. We support this using a variety of Monte-Carlo and atomistic simulations on model lipid membrane systems. However, temperature dependent fluorescence correlation spectroscopy of labeled lipids and membrane-anchored proteins in live cell membranes show a consistently smooth increase of the diffusion coefficient as a function of temperature. We find no evidence of a discrete miscibility phase transition throughout a wide range of temperatures: 14 -37 °C. This contrasts the behavior of giant plasma membrane vesicles (GPMVs) blebbed from the same cells, which do exhibit phase transitions and macroscopic phase separation. Fluorescence lifetime analysis of a DiI probe in both cases reveals a significant environmental difference between the live cell and the GPMV. Taken together, these data suggest the live cell membrane may avoid the miscibility phase transition inherent to its lipid constituents by actively regulating physical paramters, such as tension, in the membrane.

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“…Recent MD simulation results suggested that the fluorescence lifetime of DiI chromophores embedded in lipid bilayer is an effective indicator of relative membrane tension, 119 which was confirmed in experiments. 120 Indeed, fluorescence lifetime measurements of DiI-C 12 within cells demonstrated that cell membrane is less tense on softer PA gels. 117 This predicts that the cellular uptake of cells on softer PA gels or denser fibrous substrates is higher on a per membrane area basis.…”

Section: Local Mechanical Environmental Effectsmentioning

confidence: 99%

Physical Principles of Nanoparticle Cellular Endocytosis

2015

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This review article focuses on the physiochemical mechanisms underlying nanoparticle uptake into cells. When nanoparticles are in close vicinity to a cell, the interactions between the nanoparticles and the cell membrane generate forces from different origins. This leads to the membrane wrapping of the nanoparticles followed by cellular uptake. This article discusses how the kinetics, energetics, and forces are related to these interactions and dependent on the size, shape, and stiffness of nanoparticles, the biomechanical properties of the cell membrane, as well as the local environment of the cells. The discussed fundamental principles of the physiochemical causes for nanoparticle–cell interaction may guide new studies of nanoparticle endocytosis and lead to better strategies to design nanoparticle-based approaches for biomedical applications.

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Endothelial Cell Membrane Sensitivity to Shear Stress is Lipid Domain Dependent

Cited by 41 publications

References 74 publications

Effect of membrane tension on the physical properties of DOPC lipid bilayer membrane

Effect of membrane tension on the physical properties of DOPC lipid bilayer membrane

Live Cell Plasma Membranes Do Not Exhibit a Miscibility Phase Transition over a Wide Range of Temperatures

Physical Principles of Nanoparticle Cellular Endocytosis

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