Endothelial cell adhesion molecules in healthy adults during acute hyperhomocysteinemia and mild hypertriglyceridemia

Mansoor, Mohammad Azam; Seljeflot, I.; Arnesen, Harald; Knudsen, Andreas; Bates, Christopher J.; Mishra, Gita D.; Larsen, Terje H.

doi:10.1016/j.clinbiochem.2004.01.003

Cited by 28 publications

(17 citation statements)

References 38 publications

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“…Interestingly, Mansoor 21) also reported that elevated homocysteine was associated with increased adhesion molecules, a finding also observed in this study.…”

Section: Discussionsupporting

confidence: 76%

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Increased Remnant Lipoprotein in Patients with Coronary Artery Disease-Evaluation Utilizing a Newly Developed Remnant Assay, Remnant Lipoproteins Cholesterol Homogenous Assay (RemL-C)

Nakada

Kurosawa

Tohyama

et al. 2007

JAT

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Aim: Remnant lipoprotein is an emerging risk factor for coronary artery disease (CAD); however, the development of a specific remnant lipoprotein assay has struggled due to its heterogeneous nature. This study aimed to evaluate the clinical importance of a newly developed assay for remnant lipoprotein, RemL-C, in patients with CAD. Methods: This assay utilizes surfactant and phospholipase-D to selectively degrade and solubilize remnant lipoprotein. One hundred and sixty consecutive CAD patients who underwent coronary catheterization were recruited. Results: Remnant liporotein, RemL-C, was significantly higher in CAD patients (p 0.001). Additionally, TG, hs-CRP, ICAM-1, VCAM-1, and homocysteine were significantly higher, but HDL-C and adiponectin were lower with LDL-C unchanged. Since RemL-C levels correlated with plasma TG levels, two subgroups, normotriglycedemic and normolipidemic CAD groups, were extracted. In both groups, RemL-C was still significantly higher than controls. HDL-C, but not RemL-C, was associated with the severity of CAD. RemL-C significantly correlated with TG-rich lipoproteins, in particular VLDL and IDL, when limited to normolipidemic CAD patients. Conclusion: Remnant lipoprotein, measured by RemL-C, was increased in CAD patients independent of TG levels, indicating impaired remnant lipoprotein metabolism in these patients. CAD severity was associated with HDL-C, but not with remnant lipoprotein, indicating differential roles of lipoproteins in the development of coronary atherosclerosis. This study therefore provides clinical significance to assess coronary risk by measuring RemL-C, particularly among patients with normal TG levels. J Atheroscler Thromb, 2007; 14:56-64.

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“…Interestingly, Mansoor 21) also reported that elevated homocysteine was associated with increased adhesion molecules, a finding also observed in this study.…”

Section: Discussionsupporting

confidence: 76%

“…0.001), RemL-C (r 0.31, p 0.007), thus implying that homocysteine associates with lipoprotein metabolism as shown in previous studies 20,21) . Interestingly, Mansoor 21) also reported that elevated homocysteine was associated with increased adhesion molecules, a finding also observed in this study.…”

Section: Discussionsupporting

confidence: 49%

Increased Remnant Lipoprotein in Patients with Coronary Artery Disease-Evaluation Utilizing a Newly Developed Remnant Assay, Remnant Lipoproteins Cholesterol Homogenous Assay (RemL-C)

Nakada

Kurosawa

Tohyama

et al. 2007

JAT

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“…The same correlation was found in recent report among normal Brazilian children (from 1 to 8 years old) from Southeast Brazil (Alessio et al, 2004). The transient hypermocyteinaemia and mild hypertriglyceridemia have been associated to an inflammatory activation of the endothelium (Mansoor et al, 2004). An hyperhomocysteinaemia has also been described to increase thrombotic risk in hypertensive patients by increase platelet activation and decrease soluble P-selectin (Spencer et al, 2004).…”

Section: Folate Vitamin B12 and Thcy In Brazilian Newbornsupporting

confidence: 84%

Folate, vitamin B12 and total homocysteine levels in neonates from Brazil

Couto

Moreira²,

Santos

et al. 2006

Eur J Clin Nutr

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Objective: To determine folates, vitamin B12 and total homocysteine levels among neonates from mothers of low or high socioeconomic status. Design: We carried out a cross-sectional transversal study comprising 143 neonates from two maternity hospitals in the city of Salvador, Northeast of Brazil. Cord blood samples were obtained at the time of delivery from newborns from low (group 1, n ¼ 77) or high (group 2, n ¼ 66) socioeconomic status. The vitamin B12 and folates were analyzed by electrochemiluminescence immunoassay and by a competitive test using a natural folate-binding protein (FBP), respectively. Total homocyteine levels were measured by fluorescence polarization immunoassay. Maternal environmental risk factors for pregnancy complications were obtained from all mothers. Results: Only 2% of women from group 1 received prenatal care/vitamin supplementation, whereas almost all mothers from group 2 (96%) were properly followed. Anemia and/or infections pre-or during pregnancy was more prevalent among mothers of babies from group 1. Folate levels among newborns from group 1 and 2 were 7.3872.71 and 8.8374.06 ng/ml, respectively. No difference in the vitamin B12 levels was determined between groups. In addition, tHcy serum levels were higher among newborns from group 1 compared to those from group 2 (8.5474.06 vs 6.3571.33 mmol/l, respectively; P ¼ 0.005).Conclusion: These results demonstrate that unprivileged young woman has limited accesses to prenatal care, present high-risk factors that hamper both maternal and newborn health. Maternal and newborn health status could be improved by simply reinforcing the use of folate-enriched diet. The work presented illustrates the challenges that developing countries have to face in order to provide preventive adequate health care to the population at large.

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“…Acute hypertriglyceridemia induced by an oral fat load caused endothelial dysfunction in subjects with dyslipidemia 30 and increased circulatory cellular adhesion molecules in healthy subjects. 31 Further study is needed to clarify the involvement of apoCIII in this phenomenon. ApoCIII impaired insulin signaling in vascular endothelial cells and caused endothelial dysfunction at Ϸ100 g/mL in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Apolipoprotein CIII Links Hyperlipidemia With Vascular Endothelial Cell Dysfunction

et al. 2008

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Background-Apolipoprotein CIII (apoCIII) is a component of some triglyceride-rich very-low-density and low-density lipoprotein and is elevated in dyslipidemia with insulin resistance and the metabolic syndrome. We previously reported that apoCIII directly activates proinflammatory and atherogenic signaling in vascular endothelial cells through protein kinase C-␤ (PKC␤). Because PKC␤ impairs the response of vascular endothelial cells to insulin, we tested the hypothesis that apoCIII affects insulin signaling in vascular endothelial cells and its function in vitro and in vivo. Methods and Results-ApoCIII inhibited insulin-induced tyrosine phosphorylation of insulin receptor substrate 1 (IRS-1), decreasing phosphatidylinositol 3-kinase (PI3K)/Akt activation in human umbilical vein endothelial cells. These effects of apoCIII led to reduced endothelial nitric oxide synthase (eNOS) activation and NO release into the media. ApoCIII activated PKC␤ in human umbilical vein endothelial cells, resulting in IRS-1 dysfunction via serine phosphorylation. ApoCIII also activated mitogen-activated protein kinase through PKC␤. The impaired insulin signaling was restored by PKC␤ inhibitor or MEK1 inhibitor. ApoCIII-rich very-low-density lipoprotein and apoCIII impaired insulin signaling in the aorta of C57BL/6J mice and in human umbilical vein endothelial cells, which was recovered by PKC␤ inhibitor. They also inhibited endothelium-dependent relaxation of the aortas of C57BL/6J mice. In summary, apoCIII in very-low-density lipoprotein impaired insulin stimulation of NO production by vascular endothelium and induced endothelial dysfunction in vivo. This adverse effect of apoCIII was mediated by its activation of PKC␤, which inhibits the IRS-1/PI3K/Akt/eNOS pathway. Conclusion-Our

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Endothelial cell adhesion molecules in healthy adults during acute hyperhomocysteinemia and mild hypertriglyceridemia

Cited by 28 publications

References 38 publications

Increased Remnant Lipoprotein in Patients with Coronary Artery Disease-Evaluation Utilizing a Newly Developed Remnant Assay, Remnant Lipoproteins Cholesterol Homogenous Assay (RemL-C)

Increased Remnant Lipoprotein in Patients with Coronary Artery Disease-Evaluation Utilizing a Newly Developed Remnant Assay, Remnant Lipoproteins Cholesterol Homogenous Assay (RemL-C)

Folate, vitamin B12 and total homocysteine levels in neonates from Brazil

Apolipoprotein CIII Links Hyperlipidemia With Vascular Endothelial Cell Dysfunction

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