Endothelial Brg1 fine-tunes Notch signaling during zebrafish heart regeneration

Xiao, Chenglu; Hou, Junjie; Wang, Fang; Song, Yabing; Zheng, Jiyuan; Luo, Lingfei; Wang, Jianbin; Ding, Wanqiu; Zhu, Xiaojun; Xiong, Jing-Wei

doi:10.1038/s41536-023-00293-4

Cited by 4 publications

(2 citation statements)

References 72 publications

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“…BRG1 localization to promoter regions has been shown to initiate transcription following SWI/SNF-dependent chromatin remodeling [ 81 – 83 ]. There is also evidence to suggest that BRG1 can function as a transcriptional silencer [ 84 ] or regulator of H3K4me3 levels at promoters [ 85 ], highlighting the capacity of the SWI/SNF chromatin remodeling complex to exert versatile transcriptional control. BRG1 can bind both promoters and enhancers, with aberrant BAF complex activity affecting enhancer function and bivalent promoter regulation in cancers [ 86 – 93 ].…”

Section: Discussionmentioning

confidence: 99%

Chromatin profiling and state predictions reveal insights into epigenetic regulation during early porcine development

Innis,

Cabot

2024

Epigenetics & Chromatin

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Background Given their physiological similarities to humans, pigs are increasingly used as model organisms in human-oriented biomedical studies. Additionally, their value to animal agriculture across the globe has led to the development of numerous studies to investigate how to improve livestock welfare and production efficiency. As such, pigs are uniquely poised as compelling models that can yield findings with potential implications in both human and animal contexts. Despite this, many gaps remain in our knowledge about the foundational mechanisms that govern gene expression in swine across different developmental stages, particularly in early development. To address some of these gaps, we profiled the histone marks H3K4me3, H3K27ac, and H3K27me3 and the SWI/SNF central ATPase BRG1 in two porcine cell lines representing discrete early developmental time points and used the resulting information to construct predicted chromatin state maps for these cells. We combined this approach with analysis of publicly available RNA-seq data to examine the relationship between epigenetic status and gene expression in these cell types. Results In porcine fetal fibroblast (PFF) and trophectoderm cells (PTr2), we saw expected patterns of enrichment for each of the profiled epigenetic features relative to specific genomic regions. H3K4me3 was primarily enriched at and around global gene promoters, H3K27ac was enriched in promoter and intergenic regions, H3K27me3 had broad stretches of enrichment across the genome and narrower enrichment patterns in and around the promoter regions of some genes, and BRG1 primarily had detectable enrichment at and around promoter regions and in intergenic stretches, with many instances of H3K27ac co-enrichment. We used this information to perform genome-wide chromatin state predictions for 10 different states using ChromHMM. Using the predicted chromatin state maps, we identified a subset of genomic regions marked by broad H3K4me3 enrichment, and annotation of these regions revealed that they were highly associated with essential developmental processes and consisted largely of expressed genes. We then compared the identities of the genes marked by these regions to genes identified as cell-type-specific using transcriptome data and saw that a subset of broad H3K4me3-marked genes was also specifically expressed in either PFF or PTr2 cells. Conclusions These findings enhance our understanding of the epigenetic landscape present in early swine development and provide insight into how variabilities in chromatin state are linked to cell identity. Furthermore, this data captures foundational epigenetic details in two valuable porcine cell lines and contributes to the growing body of knowledge surrounding the epigenetic landscape in this species.

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Section: Discussionmentioning

confidence: 99%

Chromatin profiling and state predictions reveal insights into epigenetic regulation during early porcine development

Innis,

Cabot

2024

Epigenetics & Chromatin

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“…In this process, ECs can also release growth factors and inflammatory cytokines and interact with other cells to promote the regeneration and repair of cardiomyocytes. In one study, the cardiac endothelial Brg1-Kdm7aa-Notch axis was demonstrated to regulate myocardial proliferation and regeneration via modulating the H3K4me3 at notch promoters in zebrafish ( Xiao et al, 2023 ).…”

Section: The Extracellular Microenvironment Of Cardiomyocytes Favors ...mentioning

confidence: 99%

Effects and mechanisms of the myocardial microenvironment on cardiomyocyte proliferation and regeneration

Zheng,

Hao,

Xia

et al. 2024

Front. Cell Dev. Biol.

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The adult mammalian cardiomyocyte has a limited capacity for self-renewal, which leads to the irreversible heart dysfunction and poses a significant threat to myocardial infarction patients. In the past decades, research efforts have been predominantly concentrated on the cardiomyocyte proliferation and heart regeneration. However, the heart is a complex organ that comprises not only cardiomyocytes but also numerous noncardiomyocyte cells, all playing integral roles in maintaining cardiac function. In addition, cardiomyocytes are exposed to a dynamically changing physical environment that includes oxygen saturation and mechanical forces. Recently, a growing number of studies on myocardial microenvironment in cardiomyocyte proliferation and heart regeneration is ongoing. In this review, we provide an overview of recent advances in myocardial microenvironment, which plays an important role in cardiomyocyte proliferation and heart regeneration.

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Arsenite exposure disturbs maternal-to-zygote transition by attenuating H3K27ac during mouse preimplantation development

Sun

Huang

et al. 2023

Environmental Pollution

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Endothelial Brg1 fine-tunes Notch signaling during zebrafish heart regeneration

Cited by 4 publications

References 72 publications

Chromatin profiling and state predictions reveal insights into epigenetic regulation during early porcine development

Chromatin profiling and state predictions reveal insights into epigenetic regulation during early porcine development

Effects and mechanisms of the myocardial microenvironment on cardiomyocyte proliferation and regeneration

Arsenite exposure disturbs maternal-to-zygote transition by attenuating H3K27ac during mouse preimplantation development

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