Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs

Li, Heng; Liu, Shiqi; Hu, Yumeng; Zhao, Bin; Sun, Yeqing; Xu, Dan

doi:10.1016/j.ecoenv.2020.110267

Cited by 14 publications

(7 citation statements)

References 43 publications

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“…[14] Due to toxicity behaviour these pesticides cause various health related issues such as cancer, primary hepatocytes, seizures, gastrointestinal problems, stimulates cell migration in endothelial cells signalling pathways, endoplasmic reticulum stress, oxidative stress. [15][16][17][18] The livers cytochrome P-450 contains desulfurized ET in its active form, proved to be hazardous due to its strong inhibition of brain acetyl cholinesterase. [13] Hence, WHO (20μgL À 1 ) and the Bureau of Indian Standards (0.4μgL À 1 ) set the permissible limit of ES for drinking water.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of a Biochar‐Based Iron Oxide Catalyst for Efficient Degradation of Pesticides: Kinetics and Photoactivity

et al. 2023

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Photocatalysis is considered as promising methodology for the removal of pesticides from wastewater given its cost‐effective and environmental benignity. Pesticide toxicity and bioaccumulation have brought up environmental issues, underlining the necessity for efficient removal techniques based on novel nanomaterials. Herein, biochar (BC) synthesized by using waste peels of citrus limetta embedded with green synthesized Fe2O3 nanoparticle by using green tea extract. By using PXRD analysis, the α phase of Fe2O3 confirmed. Endosulfan (ES) and Ethion (ET) pesticides are degraded photocatalytically using a sequential, BC@ Fe2O3 nanohybrid. At the pollutant concentration (50 mg L−1), with catalytic dose (25 mg) in acidic condition under natural sunlight, BC@ Fe2O3 showed to be extremely effective in the breakdown of pesticides. The higher removal of pesticides by BC@ Fe2O3 supported by higher value of zeta potential (−22.5 mV), enhanced surface area (74 m2 g−1) and lower value of band gap (1.8 eV) as an effect of interactive characteristic and semiconducting behaviour. The fast decline in pesticide concentration followed by a gradual decrease demonstrated Ist order kinetics triggered by Langmuir adsorption. The availability of active species such as oxygen, hydroxyl radicals and holes, which are liable for pesticide breakdown, proved by scavenger analysis. Formation of safer metabolites during the photocatalytic studies confirmed by the LC‐MS studies. Finally, modified BC@ Fe2O3 nanohybrid may prove to be a viable alternative catalyst for industrial applications due to their reusability (n=7), charge separation, stability and high surface activity.

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Section: Introductionmentioning

confidence: 99%

Green Synthesis of a Biochar‐Based Iron Oxide Catalyst for Efficient Degradation of Pesticides: Kinetics and Photoactivity

et al. 2023

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“…When noxious stimuli act on cells, phosphorylated FAK or part of FAK-related signaling pathways can upregulate intracellular ROS levels, leading to abnormal cellular oxidative metabolism [ 28 , 29 ]. Y15, an FAK inhibitor, can target the Y397 site of FAK and inhibits its autophosphorylation [ 21 ], thereby regulating NO, ROS, MDA, and SOD levels [ 30 , 31 ] via inhibiting of Src/FAK, PI3K/Akt/endothelial nitric oxide synthase (eNOS), and FAK/Akt/eNOS signaling pathways [ 19 , 20 ] and resulting in reduced oxidative stress [ 32 ]. In addition, inhibiting FAK activation is also beneficial to alleviate the inflammatory damage [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Focal Adhesion Kinase Inhibitor Inhibits the Oxidative Damage Induced by Central Venous Catheter via Abolishing Focal Adhesion Kinase-Protein Kinase B Pathway Activation

Wang

Lin

Jiang

et al. 2021

BioMed Research International

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The vascular injury induced by central venous catheter (CVC) indwelling is the basis for the occurrence and development of CVC-related complications, such as phlebitis, venous thrombosis, and catheter-related infections. Focal adhesion kinase (FAK) and FAK-protein kinase B (AKT) signaling pathway are of great significance in tissue repair after trauma. Here, we investigated the role and mechanism of the FAK inhibitor (1,2,4,5-phenyltetramine tetrahydrochloride (Y15)) in oxidative damage caused by CVC. EA.hy926 cells were divided into the control group (normal control), CVCs+scratches group (the intercepted CVC segments coculturing with scratched EA.hy926 cells), and CVCs+scratches+Y15 group (Y15 was added to the cell culture supernatant with CVCs + scratches at a final concentration of 50 μmol·L-1). New Zealand rabbits were randomly divided into the control group (normal control), CVC group (CVC was inserted through the rabbit’s right jugular vein to the junction of the right atrium and superior vena cava), and CVC+Y15 group (CVC was immersed in a 50 μmol·L-1 Y15 solutions before insertion). The levels of markers and proteins related to oxidative damage in cells, cell culture supernatant, serum, and external jugular vein were measured by commercial kits and western blot, respectively. We found that Y15 treatment significantly decreased ROS and MDA levels and increased cell viability, NO, and SOD levels in a time-dependent manner in rabbit serum and cell culture supernatant. In addition, Y15 effectively reduced the CVC-induced pathological changes of damaged vascular tissues. Y15 also downregulated the levels of p-FAK Tyr 397 and p-Akt Ser 473 in damaged external jugular vein and EA.hy926 cells. These findings suggest that Y15 alleviated CVC-induced oxidative damage to blood vessels by suppressing focal FAK-Akt pathway activation.

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“…The effect of different peptides on cell migration was evaluated based on a wound healing assay. , Briefly, HUVECs were seeded on a 6-well plate at a density of 1 × 10 6 cells/well and cultured at 37 °C, 5% CO 2 . After 24 h, the confluent cell layers were gently scratched using a plastic pipette to create a scarification.…”

Section: Experimental Methodsmentioning

confidence: 99%

Surface Functionalization of Electrospun Scaffolds by QK-AG73 Peptide for Enhanced Interaction with Vascular Endothelial Cells

Bai,

Zhang,

Gao

et al. 2023

Langmuir

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Rapid endothelialization still remains challenging for blood-contacting biomaterials, especially for long-term, functional, small-diameter vascular grafts. The vascular endothelial growth factor (VEGF)-mimicking QK peptide holds great promise in promoting vascular endothelial cellular activities such as adhesion, spreading, proliferation, and migration. Syndecans are transmembrane proteoglycans that are highly expressed on cell surfaces, including vascular endothelial cells, which can act as docking receptors to provide binding sites for a variety of cellular growth and signaling molecules. Herein, a novel peptide QK-AG73 that coupled the QK domain with the syndecan binding peptide AG73 was proposed, aiming to synergistically enhance the interaction with vascular endothelial cells. In addition, mechanically matched bioactive scaffolds based on poly(L-lactide-co-εcaprolactone) were successfully prepared by surface functionalization of the covalently combined QK-AG73 peptide. The result showed that the adhesion of human umbilical vein endothelial cells (HUVECs) was increased by approximately 2-fold on QK-AG73-modified surface compared with those modified with a single QK or AG73 peptide. Moreover, surface functionalization of electrospun scaffolds by this QK-AG73 peptide was more efficient in specifically promoting the proliferation of HUVECs and allowing them to grow with an elongated cobblestone-like cell morphology. It was hypothesized that both VEGF receptors and transmembrane syndecan receptors were involved in cellular regulation by the QK-AG73 peptide, which resulted in synergistic improvement of the interactions with vascular endothelial cells and provided a promising strategy to promote endothelialization of small-diameter vascular grafts.

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Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs

Cited by 14 publications

References 43 publications

Green Synthesis of a Biochar‐Based Iron Oxide Catalyst for Efficient Degradation of Pesticides: Kinetics and Photoactivity

Green Synthesis of a Biochar‐Based Iron Oxide Catalyst for Efficient Degradation of Pesticides: Kinetics and Photoactivity

Focal Adhesion Kinase Inhibitor Inhibits the Oxidative Damage Induced by Central Venous Catheter via Abolishing Focal Adhesion Kinase-Protein Kinase B Pathway Activation

Surface Functionalization of Electrospun Scaffolds by QK-AG73 Peptide for Enhanced Interaction with Vascular Endothelial Cells

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