Endosidin 2 accelerates PIN2 endocytosis and disturbs intracellular trafficking of PIN2, PIN3, and PIN4 but not of SYT1

Lešková, Alexandra; Labajová, Mária; Krausko, Miroslav; Zahradníková, Alexandra; Baluška, Frantǐsek; Mičieta, Karol; Turña, Ján; Jásik, Ján

doi:10.1371/journal.pone.0237448

Cited by 10 publications

(11 citation statements)

References 53 publications

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“…This depletion indicates that pools of PM-localized IRK and KOIN, present prior to ES2 treatments, were degraded. Consistent with this, ES2 was shown to enhance PIN2 endocytic traffic to the lytic vacuole 32 . IRK and KOIN trafficking to the lytic vacuole is also evidenced by their retention in doughnut-shaped agglomerations induced by Wm interference with degradative trafficking (Supplementary Fig.…”

Section: Resultssupporting

confidence: 62%

“…Secretion of IRK and KOIN is sensitive to ES2 indicating the involvement of the EXOCYST subunit EXO70A1. This subunit regulates polar exocytosis of a subset of PM proteins indicating selectivity 31 , 32 . Interestingly, extended treatments with ES2 and Wm, a phosphatidylinositol 3-(PtdIns3K) and 4-(PtdIns4K) kinase inhibitor, decreased IRK and KOIN accumulation at the PM.…”

Section: Discussionmentioning

confidence: 99%

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Distinct mechanisms orchestrate the contra-polarity of IRK and KOIN, two LRR-receptor-kinases controlling root cell division

et al. 2022

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In plants, cell polarity plays key roles in coordinating developmental processes. Despite the characterization of several polarly localized plasma membrane proteins, the mechanisms connecting protein dynamics with cellular functions often remain unclear. Here, we introduce a polarized receptor, KOIN, that restricts cell divisions in the Arabidopsis root meristem. In the endodermis, KOIN polarity is opposite to IRK, a receptor that represses endodermal cell divisions. Their contra-polar localization facilitates dissection of polarity mechanisms and the links between polarity and function. We find that IRK and KOIN are recognized, sorted, and secreted through distinct pathways. IRK extracellular domains determine its polarity and partially rescue the mutant phenotype, whereas KOIN’s extracellular domains are insufficient for polar sorting and function. Endodermal expression of an IRK/KOIN chimera generates non-cell-autonomous misregulation of root cell divisions that impacts patterning. Altogether, we reveal two contrasting mechanisms determining these receptors’ polarity and link their polarity to cell divisions in root tissue patterning.

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Section: Resultssupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Distinct mechanisms orchestrate the contra-polarity of IRK and KOIN, two LRR-receptor-kinases controlling root cell division

et al. 2022

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“…For immunofluorescence, cells were seeded on coverslips and grown overnight. Treatment with 100µM endosidin-2 (Cayman, 21888) (Zhang C et al, 2016; Fujimoto et al , 2019; Leskova et al, 2020) 50µM VPS34i (Cayman, 17392), 200nM BafA1 (Sigma-Aldrich, B1793) were applied in fresh media change at implicated time and grown under standard conditions at 37°C and 5% CO 2 for the respective timepoints listed. Cells were washed three times with PBS followed by fixation for 10 minutes in 4% PFA.…”

Section: Methodsmentioning

confidence: 99%

Exocyst Inactivation in Urothelial Cells Disrupts Autophagy and Activates non-canonical NF-κB

Ortega

Villiger

Harrison-Chau

et al. 2021

Preprint

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Ureter obstruction is a highly prevalent event during embryonic development and is a major cause of pediatric kidney disease. We have reported that ureteric bud specific ablation of the exocyst Exoc5 subunit in late murine gestation results in failure of urothelial stratification, cell death, and complete ureter obstruction. However, the mechanistic connection between disrupted exocyst activity, urothelial cell death, and subsequent ureter obstruction was unclear. Here, we report that inhibited urothelial stratification does not drive cell death during ureter development. Instead, we demonstrate that the exocyst plays a critical role in autophagy in urothelial cells, and that disruption of autophagy activates a urothelial NF-κB stress response. Impaired autophagy first provokes canonical NF κB activity which is progressively followed by increasing non-canonical NF-κB activity and cell death if the stress remains unresolved. Furthermore, we demonstrate that ureter obstructions can be completely rescued in Exoc5 conditional knockout mice by administering a single dose of pan-caspase inhibitor z VAD-FMK at E16.5 prior to urothelial cell death. Taken together, ablation of Exoc5 disrupts autophagic stress response and activates progressive NF-κB signaling which promotes obstructive uropathy.

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“…The stomata were also assessed after 24 h of treatment. For microscopy analysis, we used AtSYT1-Dendra2 and AtSYT1-GFP lines as described previously [6,73,74]. Seeds were surface sterilized with 70% ethanol and 1.5% (v/v) sodium hypochlorite and germinated on the 1 2 MSMO medium (#M6899, Merck KGaA, Darmstadt, Germany) supplemented with 1% (w/v) sucrose.…”

Section: Plant Material Cultivation Conditions and Salt Treatmentmentioning

confidence: 99%

“…Five or ten-day-old seedlings were placed on a microscopic slide covered with 1.5 solidified 1 2 MSMO medium and covered by a coverslip. Photoconversion of Dendra2 was achieved using an Olympus FV1000 confocal laser scanning microscope as described previously [73,74]. Two-channel images were acquired sequentially in the multi-track mode under the same microscope using 20X Uplan FI (0.50 NA) or 40X UPLSAPO Super Apochromat (0.90) objectives.…”

Section: Confocal Microscopymentioning

confidence: 99%

The Absence of the AtSYT1 Function Elevates the Adverse Effect of Salt Stress on Photosynthesis in Arabidopsis

Krausko

Kusá

Peterková

et al. 2022

IJMS

Self Cite

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Arabidopsis thaliana SYNAPTOTAGMIN 1 (AtSYT1) was shown to be involved in responses to different environmental and biotic stresses. We investigated gas exchange and chlorophyll a fluorescence in Arabidopsis wild-type (WT, ecotype Col-0) and atsyt1 mutant plants irrigated for 48 h with 150 mM NaCl. We found that salt stress significantly decreases net photosynthetic assimilation, effective photochemical quantum yield of photosystem II (ФPSII), stomatal conductance and transpiration rate in both genotypes. Salt stress has a more severe impact on atsyt1 plants with increasing effect at higher illumination. Dark respiration, photochemical quenching (qP), non-photochemical quenching and ФPSII measured at 750 µmol m−2 s−1 photosynthetic photon flux density were significantly affected by salt in both genotypes. However, differences between mutant and WT plants were recorded only for qP and ФPSII. Decreased photosynthetic efficiency in atsyt1 under salt stress was accompanied by reduced chlorophyll and carotenoid and increased flavonol content in atsyt1 leaves. No differences in the abundance of key proteins participating in photosynthesis (except PsaC and PsbQ) and chlorophyll biosynthesis were found regardless of genotype or salt treatment. Microscopic analysis showed that irrigating plants with salt caused a partial closure of the stomata, and this effect was more pronounced in the mutant than in WT plants. The localization pattern of AtSYT1 was also altered by salt stress.

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Endosidin 2 accelerates PIN2 endocytosis and disturbs intracellular trafficking of PIN2, PIN3, and PIN4 but not of SYT1

Cited by 10 publications

References 53 publications

Distinct mechanisms orchestrate the contra-polarity of IRK and KOIN, two LRR-receptor-kinases controlling root cell division

Distinct mechanisms orchestrate the contra-polarity of IRK and KOIN, two LRR-receptor-kinases controlling root cell division

Exocyst Inactivation in Urothelial Cells Disrupts Autophagy and Activates non-canonical NF-κB

The Absence of the AtSYT1 Function Elevates the Adverse Effect of Salt Stress on Photosynthesis in Arabidopsis

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