Endoscopic ultrasound guided fine needle aspiration with fluorescence in situ hybridization analysis in 104 patients with pancreatic mass

Ribeiro, Afonso; Peng, Jinghong; Casas, Carmen; Fan, Yao Shan

doi:10.1111/jgh.12575

Cited by 19 publications

(6 citation statements)

References 14 publications

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“…In a prospective study of 104 patients, cytology plus FISH was superior to either method alone, the abnormalities detected in this study were 9p21 deletions (most common) and the polysomy of chromosome 7. FISH detected malignancies in 23 patients who had an inconclusive or negative cytology [40]. miRNA analysis has also been studied in EUS-FNA samples.…”

Section: • • Pancreatic Cystic Lesions and The Importance Of Endoscopymentioning

confidence: 98%

State-Of-The-Art Endoscopic Procedures for Pancreatic Cancer

Coronel

Waxman

2016

Future Oncol.

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Pancreatic cancer is the twelfth most common cancer worldwide, taking the fourth place in cancer-related mortality in western countries. Despite significant efforts in understanding the tumor biology of pancreatic cancer and introducing new technologies and therapies to improve the detection, staging and treatment of this disease, pancreatic cancer continues to have a high and almost unchanged mortality. In the last few decades, the development of techniques such as endoscopic retrograde cholangio pancreatography and endoscopic ultrasound have allowed us to directly access the pancreaticobiliary system and fight pancreatic cancer and its complications from different fronts. Our goal with this review is to discuss the most cutting-edge endoscopic techniques available in our armamentarium to diagnose, stage and treat pancreatic cancer.

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Section: • • Pancreatic Cystic Lesions and The Importance Of Endoscopymentioning

confidence: 98%

State-Of-The-Art Endoscopic Procedures for Pancreatic Cancer

Coronel

Waxman

2016

Future Oncol.

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“…For EUS-FNB specimen prepared by formalin-fixed, paraffinembedded, a 95% success rate for NGS is expected when samples have a tissue volume of 1.2 cm 2 and at least 5 ng/mL of analyzable DNA. 49 Although much of the initial work on EUS-TA has focused on NGS and is the focus of this paper, numerous additional methods, such as RNA and microRNA sequencing, [59][60][61][62] use of fluorescence in situ hybridization, 63,64 identification of protein and immune markers, the creation of tumor organoids, [65][66][67][68] and low cost whole-exome sequencing are emerging. Of these methods, the ability to create organoids, which are in vitro 3-dimensional tissue models, is particularly promising.…”

Section: Role Of Endoscopic Ultrasound In Personalized Medicinementioning

confidence: 99%

AGA White Paper: Optimizing Endoscopic Ultrasound–Guided Tissue Acquisition and Future Directions

Wani

Muthusamy

McGrath

et al. 2018

Clinical Gastroenterology and Hepatology

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“…Among these methods, liquid cytological sampling (FNA rinse material) is excellent and often superior for personalized medicine [61,62]. Further, RNA and microRNA sequencing [63-66], fluorescence in situ hybridization [67], use of protein and immune markers and tumor organoids [68-71], and low-cost whole-exome sequencing are currently the emerging fields in EUS-TA. Successful NGS from single circulating tumor cells was also reported [72,73].…”

Section: Future Prospect Of Eus-tamentioning

confidence: 99%

Present and Future of Endoscopic Ultrasound-Guided Tissue Acquisition in Solid Pancreatic Tumors

Park

Lee

2019

Clin Endosc

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Endoscopic ultrasound-guided tissue acquisition (EUS-TA) is a well-established method for pathological diagnosis of solid pancreatic neoplasm. It can be performed either as EUS-guided fine-needle aspiration (EUS-FNA) or EUS-guided fine-needle biopsy (EUS-FNB). The incidence of adverse events related to EUS-TA is less than 1%. The factors that affect the diagnostic accuracy and specimen adequacy include the techniques used, type and size of the needle, competency of endosonographers, presence of cytopathologists/ cytotechnologists, and rapid on-site examination. EUS-TA may contribute to precision medicine through obtaining tissue samples for next-generation sequencing. The current status, several clinical issues for diagnostic yield and adverse events, and future perspectives of EUS-FNA/FNB for diagnosing pancreatic neoplasm have been discussed in this review article.

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Endoscopic ultrasound guided fine needle aspiration with fluorescence in situ hybridization analysis in 104 patients with pancreatic mass

Abstract: In patients with inconclusive immediate cytology reading, FISH is superior to cytology and improves overall sensitivity. The 9p21 deletion is the most common abnormality seen in this cohort of patients with pancreatic cancer.

Cited by 19 publications

References 14 publications

State-Of-The-Art Endoscopic Procedures for Pancreatic Cancer

State-Of-The-Art Endoscopic Procedures for Pancreatic Cancer

AGA White Paper: Optimizing Endoscopic Ultrasound–Guided Tissue Acquisition and Future Directions

Present and Future of Endoscopic Ultrasound-Guided Tissue Acquisition in Solid Pancreatic Tumors

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