Endoreduplication in cultured mammalian cells treated with 4-nitroquinoline 1-oxide

Sutou, Shizuyo

doi:10.1016/0027-5107(73)90033-x

Cited by 11 publications

(2 citation statements)

References 19 publications

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“…As pointed out earlier, the frequency of acentric fragments induced by X-rays in GN is 4-15 times higher than that reported for several other cell types [Liang and Gaulden, 19821. Comparison of the frequency of acentric fragments induced by the four chemicals studied in the GN to that induced in other cell types by these chemicals [eg, Ohama and Kadotani, 1970;Sutou, 1973;Mace et al, 1978;Bempong, 1979;Vig, 19791 is difficult because of differences in scoring methods, treatment and recovery times relative to cell cycle times, and possibly different mitotic responses. However, the conditions of the investigation by Sutou [1973] with 4NQ0 permit a tentative comparison: The frequency of acentric fragments induced in the GN is much higher than that in Chinese hamster cells.…”

Section: Discussionmentioning

confidence: 99%

“…Comparison of the frequency of acentric fragments induced by the four chemicals studied in the GN to that induced in other cell types by these chemicals [eg, Ohama and Kadotani, 1970;Sutou, 1973;Mace et al, 1978;Bempong, 1979;Vig, 19791 is difficult because of differences in scoring methods, treatment and recovery times relative to cell cycle times, and possibly different mitotic responses. However, the conditions of the investigation by Sutou [1973] with 4NQ0 permit a tentative comparison: The frequency of acentric fragments induced in the GN is much higher than that in Chinese hamster cells. The reason for the apparent high sensitivity of the GN to induction of acentric fragments by X-rays and 4NQ0 is not understood, but we speculate that low levels of repair may partly account for it.…”

Section: Discussionmentioning

confidence: 99%

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Neuroblast of the grasshopper embryo as a new mutagen test system. Ii. Chromosome breakage induced by in vitro exposure of embryos to the direct‐acting mutagens 4NQO, MNNG, adriamycin, and bleomycin

Liang

Gaulden

1982

Environ. mutagen

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The dose-response for the induction of acentric chromosome fragments was determined in neuroblasts of the grasshopper embryo (Chortophaga viridifasciata De Geer, Orthoptera: Acrididae) exposed in vitro to four direct-acting chemical known to be mutagenic, clastogenic, and carcinogenic: 4-nitroquinoline-1-oxide (4NQO), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), Adriamycin (ADM), and bleomycin (BLM). After a 1-hr exposure followed by a 3-hr recovery period (untreated cell cycle time is 4 hr), acentric fragments were observed at doses down to 1 microM 4NQO, 1.25 microM MNNG, and 0.125 microM ADM and BLM. After an 8-hr continuous exposure, acentric fragments were induced by 4NQO at a dose as low as 0.125 microM. These low concentrations also reduced the number of dividing cells. No chromosome aberrations or mitotic effects were observed in untreated embryos or in those exposed only to the solvent dimethyl sulfoxide. Because of the short cell cycle and the sensitivity of the neuroblast to the induction of acentric chromosome fragments by chemical clastogens, a minimum of time is needed to perform the test. From a comparison with the prominent clastogen test systems currently used, it is concluded that the grasshopper neuroblast test is the fastest and that it detects some agents that some systems do not. Grasshoppers have a worldwide distribution. If neuroblasts of other species prove to be as sensitive to mutagens as those of Chortophaga, investigators in many countries would have available a eukaryotic mutagen test system that is simple, fast, reproducible, and inexpensive.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Neuroblast of the grasshopper embryo as a new mutagen test system. Ii. Chromosome breakage induced by in vitro exposure of embryos to the direct‐acting mutagens 4NQO, MNNG, adriamycin, and bleomycin

Liang

Gaulden

1982

Environ. mutagen

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Quinoline N ‐Oxides

Jones

Baty

1982

Chemistry of Heterocyclic Compounds: A Series of Monographs

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Effects of an acridine half‐mustard (ICR 191) on growth and ploidy of frog cells in culture

Viceps-Madore

1978

Journal Cellular Physiology

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The effects of an acridine half-mustard, ICR 191, on the growth rate and ploidy of four haploid and two diploid lines of Rana pipiens cells in culture were studied. Growth curves indicate that the haploid and diploid cell lines were equally resistant to a 4-hour exposure of this drug (0.1 micrometer to 10 micrometer. ICR 191 treatment induced the haploid cell cultures to become diploid. The proportion of diploid cells increased progressively with respect to time after the 4-hour exposure period. The greater the concentration of ICR 191 applied, the more rapid the rate of conversion. Autoradiographic determinations of percent labelled nuclei indicate that DNA synthesis was not inhibited in haploid or in diploid cells. Therefore, the increased proportion of diploid cells did not originate from the small percentage of diploid cells in the initial population. Instead the haploid cells were converted to diploid cells. Time lapse cinematography indicated that the conversion mechanism was other than cell fusion. Conversion to higher ploidy did not occur when diploid cell cultures were exposed to ICR 191.

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Endoreduplication in cultured mammalian cells treated with 4-nitroquinoline 1-oxide

Cited by 11 publications

References 19 publications

Neuroblast of the grasshopper embryo as a new mutagen test system. Ii. Chromosome breakage induced by in vitro exposure of embryos to the direct‐acting mutagens 4NQO, MNNG, adriamycin, and bleomycin

Neuroblast of the grasshopper embryo as a new mutagen test system. Ii. Chromosome breakage induced by in vitro exposure of embryos to the direct‐acting mutagens 4NQO, MNNG, adriamycin, and bleomycin

Quinoline N ‐Oxides

Effects of an acridine half‐mustard (ICR 191) on growth and ploidy of frog cells in culture

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