Endoplasmic reticulum: Where nucleotide sugar transport meets cytokinin control mechanisms

Niemann, Michael C E; Werner, Tomáš

doi:10.1080/15592324.2015.1072668

Cited by 4 publications

(5 citation statements)

References 36 publications

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“…Many membrane proteins, such as those belonging to the SLC family of transporters, have no known function 31 . In vitro functional assays are the gold-standard, but can be technically difficult to establish and are often hindered by the unavailable of suitable radiolabeled ligands for biochemical assays 31 , 32 . We, therefore, decided to evaluate if the GFP-TS could also encompass the screening of small molecule ligands to aid the functional characterization of membrane proteins.…”

Section: Resultsmentioning

confidence: 99%

“…As a test-case, we screened possible substrates to detergent solubilized membranes harboring an uncharacterized plant transporter from Zea mays that shares ~28% sequence identity to the human nucleotide-sugar transporter (SLC35A1), which transports CMP–sialic acid into the ER and Golgi lumen in exchange for CMP for sialyation of secretory proteins 33 , 34 . In this case, it was unclear if CMP–sialic acid was really a substrate for the plant protein as nucleotide-sugar transporters can have overlapping substrate specificities and even members with high sequence identity can transport different substrates 32 , 34 . Using GFP-TS we could however observe clear and specific thermostabilization upon the addition of CMP, which is a counter-substrate unique to the nucleotide-sugar transporters that transport CMP–sialic acid (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…An obvious advantage compared to thermal-shift assays using thermofluor dyes is that the membrane proteins do not need to be purified first, and potential interactions can be screened in the presence of their native lipids, which can be critical for the reliable identification of in vivo ligands 40 . Indeed, we could use the GFP-TS assay to establish the substrate specificity of a plant nucleotide-sugar transporter homolog of human SLC35A1, which belongs to a family that despite its role in human diseases, to date, has proved a considerable challenge to characterize 32 . Indeed, many SLC transporters have no known function, which is an important issue since close to half of them are associated with human disease and are important drug targets 31 , 41 .…”

Section: Discussionmentioning

confidence: 99%

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An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins

et al. 2018

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Membrane bilayers are made up of a myriad of different lipids that regulate the functional activity, stability, and oligomerization of many membrane proteins. Despite their importance, screening the structural and functional impact of lipid–protein interactions to identify specific lipid requirements remains a major challenge. Here, we use the FSEC-TS assay to show cardiolipin-dependent stabilization of the dimeric sodium/proton antiporter NhaA, demonstrating its ability to detect specific protein-lipid interactions. Based on the principle of FSEC-TS, we then engineer a simple thermal-shift assay (GFP-TS), which facilitates the high-throughput screening of lipid- and ligand- interactions with membrane proteins. By comparing the thermostability of medically relevant eukaryotic membrane proteins and a selection of bacterial counterparts, we reveal that eukaryotic proteins appear to have evolved to be more dependent to the presence of specific lipids.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins

et al. 2018

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“…Interestingly, the localization pattern of SvSWEET4 in the xylem parenchyma was atypical of localization to the plasma membrane and possibly more reminiscent of detection in the endoplasmic reticulum (ER) or Golgi body. These organelles are essential for facilitating the transfer of sugars to proteins, lipids, proteoglycans, and polysaccharides of the plant cell wall ( Niemann and Werner, 2015 ). Consequently, SvSWEET4 could aid in this process given it was found in highly lignified tissue structures of the stem and pedicel ( Figs 2 , 3 ).…”

Section: Discussionmentioning

confidence: 99%

The role of SWEET4 proteins in the post-phloem sugar transport pathway of Setaria viridis sink tissues

Chen

Ganguly

Shafik

et al. 2023

Journal of Experimental Botany

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In the developing seeds of all higher plants, filial cells are symplastically isolated from the maternal tissue supplying photosynthate to the reproductive structure. Photoassimilates must be transported apoplastically, crossing several membrane barriers; a process facilitated by sugar transporters. Sugars Will Eventually be Exported Transporters (SWEETs) have been proposed to play a crucial role in apoplastic sugar transport during phloem unloading and the post-phloem pathway in sink tissues. Evidence for this is presented here for developing seeds of the C4 model grass Setaria viridis. Using immunolocalisation, SvSWEET4 was detected in various maternal and filial tissues within the seed along the sugar transport pathway, in the vascular parenchyma of the pedicel and the xylem parenchyma of the stem. Expression of SvSWEET4a in Xenopus laevis oocytes indicated that they function as high-capacity glucose and sucrose transporters. Carbohydrate and transcriptional profiling of Setaria seed heads showed that there were some developmental shifts in hexose and sucrose levels and consistent expression of SvSWEET4 homologues. Collectively, these results provide evidence for the involvement of SWEETs in the apoplastic transport pathway of sink tissues and allow a pathway for post-phloem sugar transport into the seed to be proposed.

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“…CK biosynthesis is noticeably concomitant with SUT gene expression during pea seed germination. Therefore, we may wonder whether sugar transporters could be a convergent target of sugars and CKs during this process [ 86 ].…”

Section: Seed Development Germination and Seedling Establishmentmentioning

confidence: 99%

Convergence and Divergence of Sugar and Cytokinin Signaling in Plant Development

Wang

Gourrierec

Jiao

et al. 2021

IJMS

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Plants adjust their growth and development through a sophisticated regulatory system integrating endogenous and exogenous cues. Many of them rely on intricate crosstalk between nutrients and hormones, an effective way of coupling nutritional and developmental information and ensuring plant survival. Sugars in their different forms such as sucrose, glucose, fructose and trehalose-6-P and the hormone family of cytokinins (CKs) are major regulators of the shoot and root functioning throughout the plant life cycle. While their individual roles have been extensively investigated, their combined effects have unexpectedly received little attention, resulting in many gaps in current knowledge. The present review provides an overview of the relationship between sugars and CKs signaling in the main developmental transition during the plant lifecycle, including seed development, germination, seedling establishment, root and shoot branching, leaf senescence, and flowering. These new insights highlight the diversity and the complexity of the crosstalk between sugars and CKs and raise several questions that will open onto further investigations of these regulation networks orchestrating plant growth and development.

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Endoplasmic reticulum: Where nucleotide sugar transport meets cytokinin control mechanisms

Cited by 4 publications

References 36 publications

An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins

An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins

The role of SWEET4 proteins in the post-phloem sugar transport pathway of Setaria viridis sink tissues

Convergence and Divergence of Sugar and Cytokinin Signaling in Plant Development

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