Endoplasmic membrane degranulation<i>in vivo</i> as a result of ethionine intoxication

Williams, David; Rabin, B. R.; Kisilevsky, Robert

doi:10.1016/0014-5793(72)80583-0

Cited by 13 publications

(2 citation statements)

References 15 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The livers were shredded, homogenized and subfractionated into total microsomal membrane fraction, rough and smooth surfaced subfractions, and a free" polysome preparation by centrifugation on discontinuous sucrose gradients as described previously (Williams, Gurari and Rabin, 1969;Williams and Rabin, 1969;Williams, Rabin and Kisilevsky, 1972).…”

Section: Methodsmentioning

confidence: 99%

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

Williams¹,

Clark²,

Rabin³

1973

Br J Cancer

View full text Add to dashboard Cite

Summary.-The effects of aflatoxin B1 on the endoplasmic reticulum of rat liver has been examined in vivo. Electron microscopy has shown a disorganization and degranulation of rough surfaced membrane under these conditions and evidence is presented that this is a primary effect of the toxin, and results from the direct attack of the aflatoxin on the steroid-dependent ribosome binding sites on the membrane. A technique is described by which the presence of degranulated rough membrane may be detected in microsomal preparations.

show abstract

Section: Methodsmentioning

confidence: 99%

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

Williams¹,

Clark²,

Rabin³

1973

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…All of the potential ribosome-binding sites on the smooth membranes are destroyed on feeding the toxin for just two days at the same level. In vivo experiments with another carcinogen, ethionine (Williams, Rabin, and Kisilevsky, 1972), also showed substantial degranulation of rough reticular membranes. Unlike aflatoxin, ethionine produces a lesion in the ribosomes which makes them incapable ofinteracting with membranes from control animals.…”

mentioning

confidence: 95%

The effects of steroid hormones and carcinogens on the interaction of membranes with polysomes

Rabin¹,

Blyth²,

Doherty³

et al. 1974

Journal of Clinical Pathology

View full text Add to dashboard Cite

Ethionine induced degeneration and regeneration in the rat parotid gland: An electron microscope study

Leeb

1975

Am. J. Anat.

View full text Add to dashboard Cite

The object of the present study was to establish a model for the study of parotid gland regeneration. Adult female Spague-Dalley rats were placed for 11 days on a protein-free diet with daily intraperitoneal doses of aqueous DL-ethionine equivalent to 0.5 hg/gm body weight, and returned to a normal diet on day 12. At varying intervals, both during and after intoxication, animals were sacrificed and the parotid glands prepared for study with the transmission electron microscope. Ultrastructural observations indicated that damage was essentially limited to the acinar cells, in which the protein synthetic apparatus was the focus of injury. The rough endoplasmic reticulum displayed atypical configurations, loss of attached ribosomes and membrane fragmentation. In the Golgi region, an atypical structure, a "crystalloid" arose during intoxication. Because of the morphology and apparent formation of the "crystalloid," it was assumed to be an abnormal secretion product. Resumption of a normal diet resulted in the rapid restitution of the normal cytoarchitecture. During the first week of recovery, there was prominent mitotic activity in mature acinar cells. It was concluded that the primary effect of ethionine upon the parotid gland is interference with the function of the protein-synthetic apparatus, leading to morphologic alteration of the acinar cells. The mitotic activity observed during recovery indicated that in the adult rat, acinar cells retain the potential for proliferation.

show abstract

Endoplasmic membrane degranulationin vivo as a result of ethionine intoxication

Cited by 13 publications

References 15 publications

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

The effects of steroid hormones and carcinogens on the interaction of membranes with polysomes

Ethionine induced degeneration and regeneration in the rat parotid gland: An electron microscope study

Contact Info

Product

Resources

About