Endophytism and endolichenism in Pezizomycetes: the exception or the rule?

Healy, Rosanne; Arnold, A. Elizabeth; Bonito, Gregory; Huang, Yu‐Ling; Lemmond, Benjamin; Pfister, Donald H.; Smith, Matthew E.

doi:10.1111/nph.17886

Cited by 13 publications

(11 citation statements)

References 70 publications

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“…Alignment was carried out based on the taxonomic sampling method employed by Argnello et al [ 26 ] and Healy et al [ 24 ] to explore the phylogenetic relationships of the new species of Wolfina ( Table 5 ). The ITS region was aligned using the online version of MAFFT v.7 [ 18 , 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

New Ascomycetes from the Mexican Tropical Montane Cloud Forest

Raymundo,

Valenzuela,

Martínez-González

et al. 2023

JoF

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The tropical montane cloud forest is the most diverse and threatened vegetation type in Mexico. In the last decade, the number of described Ascomycetes species has notably increased, reaching more than 1300 species. This study describes six new species based on their molecular and morphological characteristics. Our results suggest that Mexico has the highest number of described species in the Neotropics. However, many other Mexican lineages still need to be described.

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Section: Methodsmentioning

confidence: 99%

New Ascomycetes from the Mexican Tropical Montane Cloud Forest

Raymundo,

Valenzuela,

Martínez-González

et al. 2023

JoF

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“…A subset of specimens was successfully cultured by sampling a piece of hymenium, placing it in sterile water, agitating the tissue to release ascospores, plating serial dilutions of the ascospore liquid on potato dextrose agar (PDA) and malt extract agar (MEA) with penicillin and streptomycin, and isolating single germinating ascospores onto PDA and MEA without antibiotics. In addition, a set of Gyromitra cultures from a recent study on Pezizales fungi were provided by Dr. A. Elizabeth Arnold from the living collection at the Robert L. Gilbertson Mycological Herbarium at the University of Arizona (Healy et al 2022). Two Gyromitra cultures whose genomes have been sequenced were also purchased from the Westerdijk Fungal Biodiversity Institute CBS culture collection.…”

Section: Methodsmentioning

confidence: 99%

“…Elizabeth Arnold from the living collection at the Robert L. Gilbertson Mycological Herbarium at the University of Arizona (Healy et al 2022). Two Gyromitra cultures whose genomes have been sequenced were also purchased from the Westerdijk Fungal Biodiversity Institute CBS culture collection.…”

Section: Methodsmentioning

confidence: 99%

Distribution of the gyromitrin mycotoxin in the lorchel family assessed by a pre-column-derivatization and ultra high-performance liquid chromatography method

Dirks

Mohamed

Schultz

et al. 2022

Preprint

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Gyromitrin (acetaldehyde N-methyl-N-formylhydrazone) and its homologs are deadly mycotoxins produced most infamously by the lorchel (also known as false morel) Gyromitra esculenta, which is paradoxically consumed as a delicacy in some parts of the world. There is much speculation about the presence of gyromitrin in other species of the lorchel family (Discinaceae), but no studies have broadly assessed its distribution. Given the history of poisonings associated with the consumption of G. esculenta and G. ambigua, we hypothesized that gyromitrin evolved in the last common ancestor of these taxa and would be present in their descendants with adaptive loss of function in the nested truffle clade, Hydnotrya. To investigate this hypothesis, we developed a sensitive analytical derivatization method for the detection of gyromitrin using 2,4-dinotrobenzaldehyde as the derivatization reagent. In total, we analyzed 66 specimens for the presence of gyromitrin over 105 tests. Moreover, we sequenced the nuc rDNA ITS1-5.8S-ITS2 region (ITS barcode) and nuc 28S rDNA to assist in species identification and to infer a supporting phylogenetic tree. We detected gyromitrin in all tested specimens from the G. esculenta group as well as G. leucoxantha. This distribution is consistent with a model of rapid evolution coupled with horizontal transfer, which is typical for secondary metabolites. We clarified that gyromitrin production in Discinaceae is both discontinuous and more limited than previously thought. Further research is required to elucidate the gyromitrin biosynthesis gene cluster and its evolutionary history in lorchels.

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“…delimitation, our morphological examination revealed no signi cant characters to distinguish G. americanigigas, G. gigas and G. ussuriensis. Attempts to culture species in the G. gigas complex failed and we are unaware of anyone else successfully doing so (Healy et al 2022), preventing the use of intercompatibility tests to facilitate the delimitation of species boundaries under the biological species concept. The LSU cannot be used a barcode marker since the infra-and interspeci c variation among sequences of these three species was nearly identical.…”

Section: Sequence Similarity Comparisonsmentioning

confidence: 99%

Studies in Gyromitra II: cryptic speciation in the Gyromitra gigas species complex; rediscovery of G. ussuriensis and G. americanigigas sp. nov.

et al. 2022

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Taxa in the Gyromitra gigas species complex were previously studied and their taxonomy resolved. During ongoing studies in this group, cryptic speciation was discovered in G. gigas. Sequences of the ITS and LSU regions from 75 specimens were included in maximum likelihood and Bayesian phylogenetic analyses to establish species boundaries and resolve species relationships. Sequence similarity comparisons were also conducted between the two ribosomal markers and between the ITS1 and ITS2 regions. Gyromitra gigas sensu stricto and two additional species were discovered within the G. gigas clade. Gyromitra ussuriensis was rediscovered as a distinct taxon and removed from synonymy under G. gigas. It occurs in central and eastern Asia, whereas G. gigas occurs mostly in Europe but also extends into central Asia. A neotype is designated for G. ussuriensis. A new species, Gyromitra americanigigas, is described and illustrated from eastern North America. Although morphology and the LSU exhibited little variation among the three species, the ITS1 and ITS2 regions displayed similar interspeci c sequence variability around 0.5-1%, which is su cient for species identi cation at the molecular level.

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Endophytism and endolichenism in Pezizomycetes: the exception or the rule?

Cited by 13 publications

References 70 publications

New Ascomycetes from the Mexican Tropical Montane Cloud Forest

New Ascomycetes from the Mexican Tropical Montane Cloud Forest

Distribution of the gyromitrin mycotoxin in the lorchel family assessed by a pre-column-derivatization and ultra high-performance liquid chromatography method

Studies in Gyromitra II: cryptic speciation in the Gyromitra gigas species complex; rediscovery of G. ussuriensis and G. americanigigas sp. nov.

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