Endophytic yeasts in Malus domestica and Pyrus communis fruits under anthropogenic impact

Глушакова, А. М.; Качалкин, А. В.

doi:10.1134/s0026261716060102

Cited by 61 publications

(43 citation statements)

References 24 publications

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“…Filobasidium magnum was found to be present on V. vinifera grape berries but not in the unfiltered wine ( Kačániová et al, 2020 ) indicating that this species could play a role before alcoholic fermentation begins. In addition, F. magnum has also been isolated from apples and pears ( Glushakova and Kachalkin, 2017 ). While previous studies have demonstrated that F. magnum is a ubiquitous in vineyards and on grape berries, the impact of F. magnum on production of wine volatile metabolites is not known.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Microbial Dynamics and Volatile Metabolome Changes During Fermentation of Chambourcin Hybrid Grapes From Two Pennsylvania Regions

et al. 2021

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Microbial diversity present on grapes in wineries, and throughout fermentation has been associated with important metabolites for final wine quality. Although microbiome-metabolome associations have been well characterized and could be used as indicators of wine quality, the impact of regionality on the microbiome and metabolome is not well known. Additionally, studies between microbiome and metabolome have been conducted on single species grape such as Vitis vinifera instead of other species and interspecific hybrids. Although the Pennsylvania wine industry is relatively young compared to California, the industry has been experiencing rapid growth over the past decade and is expected to continue to grow in the future. Pennsylvania’s climate of cold winters and high levels of rainfall throughout the growing season favors cultivation of interspecific hybrid grapes such as Vitis ssp. Chambourcin, one of the most commonly grown hybrid varieties in the state. Chambourcin is a prime candidate for studying the impact of regionality on microbiome-metabolome interactions as interspecific hybrid varieties could shape the future of winemaking. Here, we identify for the first time the regional distribution of microbial communities and their interactions with volatile metabolome during fermentation (0–20 days) by integrating high throughput Illumina sequencing (16S and ITS) and headspace-solid phase microextraction-gas chromatography-mass spectrometry. Analyzing 88 samples from nine wineries in the Central and East Pennsylvania regions, we observed high microbial diversity during early stages of fermentation (1–4 days) where non-Saccharomyces yeasts such as Starmerella and Aureobasidium and non-Oenococcus bacteria, Sphingomonas, likely contribute to microbial terroir to the resulting wines. Furthermore, key differentiators between two regions in Pennsylvania, as identified by LEfSe analysis, include the fungal genera Cladosporium and Kazachstania and the bacterial genera Lactococcus and Microbacterium. Moreover, 29 volatile fermentation metabolites were discriminated significantly (variable importance in projection > 1) between the two regions as shown by Partial Least Squares-Discriminant Analysis. Finally, Spearman’s correlation identified regional differences of microbial-metabolite associations throughout fermentation that could be used for targeted microbiome manipulation to improve wine quality and preserve regionality. In summary, these results demonstrate the microbial signatures during fermentation and differential microorganisms and metabolites further support impact of regionality on Chambourcin wines in Pennsylvania.

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Section: Discussionmentioning

confidence: 99%

Characterization of Microbial Dynamics and Volatile Metabolome Changes During Fermentation of Chambourcin Hybrid Grapes From Two Pennsylvania Regions

et al. 2021

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“…The enumeration of yeasts from coconuts revealed previously known yeast numbers in plant tissue in the range of 10 2 -10 4 c.f.u. g −1 [22][23][24]. On the other hand, among the species from coconuts, only K. ohmeri was previously isolated as an endophyte [25].…”

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confidence: 99%

“…Yeasts from fruits and plant tissues have been isolated from many countries [32]. During investigation of the diversity of endophytic yeasts in tropical regions, numerous new yeast species were obtained [24,33,34], among them some species from the Yamadazyma clade i.e. Y. endophytica and Y. riverae which are able to live as endophytes [13,34].…”

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confidence: 99%

Yamadazyma cocois f.a., sp. nov., an ascomycetous yeast isolated from coconuts

Maksimova

Глушакова

Thanh³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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During studies on the endophytic yeast communities associated with fruits from Vietnam, three fermenting yeast strains were isolated from fruits of the coconut palm (Cocos nucifera). Phylogenetic analysis based on the sequences of the ITS regions and D1/D2 domains of the large subunit rRNA gene showed that these strains represented a single species of the Yamadazyma clade that was distinct from the other related species. The new species represented a basal branch of the clade formed by the Yamadazyma species i.e. Y. insecticola and Y. takamatsuzukensis. Based on the phylogenetic analysis and phenotypic characteristics, the studied strains were assigned to a novel species of the genus Yamadazyma, for which the name Yamadazyma cocois f.a., sp. nov. is proposed. The holotype is VCIM 4241, with the ex-type cultures VTCC 920004=VKM Y-3049=KBP Y-6091 code 17–68. The MycoBank number is MB 834435.

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“…Filobasidium species were not reported in studies of fungal endophytes that used culture‐dependent methods. With NGS technique, Filobasidium endophytes were detected in Malus domestica and Pyrus communis fruits in low occurrence (Glushakova and Kachalkin, 2017), and as a dominant group in wheat populations in Italy (Casini et al ., 2019) and Israel (our unpublished data). These studies all indicate that Filobasidium spp.…”

Section: Discussionmentioning

confidence: 99%

Significant host‐ and environment‐dependent differentiation among highly sporadic fungal endophyte communities in cereal crops‐related wild grasses

Sun

Kosman

Sharon

et al. 2020

Environmental Microbiology

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Summary Endophytic fungi compose a significant part of plant microbiomes. However, while a small number of fungal taxa have proven beneficial impact, the vast majority of fungal endophytes remain uncharacterized, and the drivers of fungal endophyte community (FEC) assembly are not well understood. Here, we analysed FECs in three cereal crops‐related wild grasses – Avena sterilis, Hordeum spontaneum and Aegilops peregrina – that grow in mixed populations in natural habitats. Taxa in Ascomycota class Dothideomycetes, particularly the genera Alternaria and Cladosporium, were the most abundant and prevalent across all populations, but there was also high incidence of basidiomyceteous yeasts of the class Tremellomycetes. The fungal community was shaped to large extent by stochastic processes, as indicated by high level of variation even between individuals from local populations of the same plant species, and confirmed by the neutral community model and Raup‐Crick index. Nevertheless, we still found strong determinism in FEC assembly with both incidence and abundance data sets. Substantial differences in community composition across host species and locations were revealed. Our research demonstrated that assembly of FECs is affected by stochastic as well as deterministic processes and suggests strong effects of environment heterogeneity and plant species on community composition. In addition, a small number of taxa had high incidence and abundance in all of the 15 populations. These taxa represent an important part of the core FEC and might be of general functional importance.

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Endophytic yeasts in Malus domestica and Pyrus communis fruits under anthropogenic impact

Cited by 61 publications

References 24 publications

Characterization of Microbial Dynamics and Volatile Metabolome Changes During Fermentation of Chambourcin Hybrid Grapes From Two Pennsylvania Regions

Characterization of Microbial Dynamics and Volatile Metabolome Changes During Fermentation of Chambourcin Hybrid Grapes From Two Pennsylvania Regions

Yamadazyma cocois f.a., sp. nov., an ascomycetous yeast isolated from coconuts

Significant host‐ and environment‐dependent differentiation among highly sporadic fungal endophyte communities in cereal crops‐related wild grasses

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