Endophytic Microorganisms of the Tropical Savannah: A Promising Source of Bioactive Molecules

Sousa, Cristina Paiva de; Serrano, Nadja Fernanda Gonzaga; Lacava, Paulo Teixeira

doi:10.1007/978-3-319-55804-2_4

Cited by 6 publications

(3 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies in the Pantanal and Cerrado are usually on animal and plant biodiversity (Hyde and Soytong, 2008 ; Porras-Alfaro and Bayman, 2011 ). A very limited number of studies in these biomes are conducted on endophytic microorganisms, and most of them are focused on aspects of bioprospecting without community analysis (Carvalho et al, 2012 ; Glienke et al, 2012 ; Vieira et al, 2014 ; Savi et al, 2016 ; Gos et al, 2017 ; Hokama et al, 2017 ; Parpinelli et al, 2017 ; Sousa et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

et al. 2018

View full text Add to dashboard Cite

Medicinal plants have been recognized as hosts of high diverse endophytic microorganisms, including fungi that produce secondary metabolites with biological activity. Two biomes in Brazil, Pantanal (wetland), and Cerrado (savannah), are known as biodiversity hotspots, and despite their importance as a reservoir for several species, knowledge about the fungal biodiversity in these biomes is very limited. Fungal endophytic communities associated with leaves and petioles of the medicinal plants Vochysia divergens (from Pantanal) and Stryphnodendron adstringens (from Cerrado) were analyzed and studied for their antimicrobial activity against human and plant pathogens. A total of 1,146 isolates of endophytic fungi were obtained from plants collected in January and June of 2016 and grouped into 124 morphotypes. One isolate of each morphotype was identified by sequencing of internal transcribed spacer (ITS) region of the rDNA gene, which revealed the presence of 24 genera, including 3 possible new genera, and 48 taxa. Differences in the endophytic community according to the biomes were observed concerning the analyzed morphotypes. However, when we analyzed the diversity of genera and richness, they were similar for both plants, with Diaporthe, Phyllosticta, and Neofusicoccum as dominant genera. In addition, the community composition of V. divergens differs according to the analyzed plant tissues (petiole and leaf). These data suggested that both, the plant species and plant tissues play a role in the composition of endophytic community. As regards the biotechnological potential, 5 isolates showed activity against the phytopathogens Phyllosticta citricarpa, Colletotrichum abscissum, and Fusarium verticilioides, and 8 isolates showed high activity against clinical pathogens and were selected for the production of crude extract in different culture media. Extract from cultivation of Diaporthe sp. LGMF1548 and LGMF1583 and Neofusicoccum brasiliense LGMF1535 showed activity against methicillin-resistant Staphylococcus aureus, Klebssiella pneumonia, and Candida albicans. In addition, extracts of Diaporthe cf. heveae LGMF1631 inhibited 90% of the mycelial growth of the P. citricarpa and 70% of C. abscissum and may represent an alternative to be used in the biological control of these phytopathogens. Future research will focus on the chemical characterization and structural elucidation of these bioactive compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

et al. 2018

View full text Add to dashboard Cite

show abstract

“…al, 2010). Boa parte desta riqueza de espécies nativas é encontrada no mosaico de biomas do Cerrado, que atualmente por meio dos avanços das fronteiras agrícolas está entre os 25 hotspots mundiais, que são conjuntos de ecorregiões prioritárias para conservação em nível mundial (SOUSA; SERRANO;LACAVA, 2017).…”

Section: Introductionunclassified

Bioprospecção e potencial biotecnológico de fungos endofíticos associados a plantas do Cerrado

Torres¹,

Bogas²,

Andrade³

et al. 2022

CLIUM

Self Cite

View full text Add to dashboard Cite

Os microrganismos endofíticos são fungos ou bactérias que colonizam o interior dos tecidos vegetais sem causar dano à planta hospedeira. Os fungos endofíticos têm interessado a comunidade científica devido à produção de metabolitos secundários com aplicação nas indústrias biotecnológicas e farmacêuticas. Desta forma, o presente estudo objetivou isolar, purificar, identificar genotipicamente e avaliar a atividade antagônica, o potencial biotecnológico para solubilização fosfato e produção de enzimas de 66 fungos endofíticos de folhas e ramos isolados de S. adstringens e S. lycocarpum St. Hill. Para isso, folhas e caules saudáveis de S. adstringens e S. lycocarpum foram coletadas e submetidas à assepsia superficial. Após a incubação do material botânico procedeu-se o isolamento, identificação molecular dos fungos endofíticos. Posteriormente, os isolados endofíticos foram avaliados in vitro quanto ao potencial antagônico contra os fitopatógenos Colletotrichum sp., Fusarium oxysporum e Lasiodiplodia subglobosa, quanto a solubilização de fosfato inorgânico e a produção de enzimas (amilase, celulase, pectinase, protease, lipase e esterase). Os resultados mostraram que a comunidade endofítica fúngica associada à S. adstringens é composta por: Diaporthe sp., Neopestalotiopsis sp., Nigrospora sp., Paraconiothrium sp., Phomopsis sp., Pseudofusicoccum sp., Schizophyllum sp. e Xenoacremonium sp. e os gêneros da comunidade fúngica de S. lycocarpum St. Hill. é composta por: Colletotrichum sp., Curvalaria sp., Diaporthe sp., Neurospora sp., Phlebiopsis sp., Phomopsis sp. e Schizophyllum sp.. Os fungos endofíticos se mostraram promissores contra Colletotrichum sp., Fusarium oxysporum e Lasiodiplodia subglobosa sendo observados elevados índices de inibição e a maioria das interações do tipo A, onde há um bloqueio de crescimento do fitopatógeno com contato micelial do fungo endofítico. Além disso, os fungos endofíticos apresentaram potencial de solubilizar fosfato inorgânico com índice de solubilização de fosfato médio. Quanto à produção de enzimas, os isolados fúngicos de ambas as espécies vegetais apresentaram maior atividade pectinolítica com produção de pectato liase e a poligalactorose. Com base nos resultados o estudo evidencia a importância dos fungos endofíticos na produção de compostos de interesse para biotecnologia e para a conservação das espécies vegetais do Cerrado, as quais abrigam esses micro-organismos promissores.

show abstract

“…Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License microorganisms. Much of the biodiversity of this microbiome is not yet known, which makes it one of the 25 most important terrestrial biodiversity hotspots on the planet (Oliveira-Filho and Ratter, 2002;Sousa et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Isolation and in vitro screening of plant growth-promoting rhizobacteria from Solanum lycocarpum St. Hil., an endemic plant of the Brazilian tropical savannah

Lisboa¹,

Andrade²,

Machado³

et al. 2021

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Solanum lycocarpumSt. Hil. is an endemic plant of the Brazilian tropical savannah (cerrado) that is capable of growing on acidic and nutrient-poor land, an ability which attracts attention to its rhizospheric microbiota, including plant growth-promoting rhizobacteria (PGPR). In this work, 131 bacterial strains were isolated from rhizosphere samples of S. lycocarpum and were tested in vitro for direct mechanisms of plant growth promotion (biological nitrogen fixation, phosphate solubilization and indolic compounds production) and enzyme activities. The 26 most promising isolates selected from the previous tests were used to continue the screening. Ten of these isolates showed antifungal activity against fourteen phytopathogenic fungi and twelve isolates showed antimicrobial activity against at least one of the three clinical pathogens evaluated. Seven of the 26 isolates were identified at random as belonging to the genera Bacillus, Burkholderia and Microbacterium, are PGPR and have potential to participate in more in-depth research aimed at the development of bio-inputs, especially the Bacillus strains.

show abstract

Endophytic Microorganisms of the Tropical Savannah: A Promising Source of Bioactive Molecules

Cited by 6 publications

References 52 publications

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

Bioprospecção e potencial biotecnológico de fungos endofíticos associados a plantas do Cerrado

Isolation and in vitro screening of plant growth-promoting rhizobacteria from Solanum lycocarpum St. Hil., an endemic plant of the Brazilian tropical savannah

Contact Info

Product

Resources

About