Endophytic microflora from Ficus carica L. leaves – isolation, characterization and potential for application

Abstract

Fig leaves (Ficus carica L.) are widely used in traditional medicine as a remedy or for prevention of many health problems (lowering blood sugar and triglyceride levels, cardiovascular diseases, etc.). The aim of the research was isolation of endophytic microflora, its characterization and proving its potential for future application. Two endophytic bacteria Streptococcus sp. Fcl1 and Kocuria rhizophila Fcl20 were isolated from the fig leaves and characterized. Using HPLC method was also determined the polyphenolic profile of aqueous-alcoholic extract (70% (v/v) ethanol) and microwave-assisted aqueous extract of fig leaves, for the purpose of phytochemical characterization of the plant, for subsequent study of the endophyte-plant relationship. The main found phenolic acids and flavonoids in extracts were: (+)-catechin, vanillic acid, syringic acid, (-)-epicatechin, ferulic acid, salicylic acid, rosmarinic acid, rutin.