Endophytic Microbiota Comparison of Dendrobium huoshanense Root and Stem in Different Growth Years

Abstract

The endophytic microbiome in medicinal plants is rich and diverse, but few studies have followed the endophytic microbiome of medicinal plants in different tissues with their growth. In this study, we examined the endophytic bacterial and fungal community structures associated with both the stem and root compartments of Dendrobium huoshanense at different growth years via high-throughput sequencing of 16S rRNA genes and nrDNA fragments of internal transcribed spacer regions. Results indicated that more diverse prokaryotic and fungal operational taxonomic units were detected in roots than in stems, and the alpha diversity of endophytic prokaryotic significantly differed among the 1-, 2-, and 3-year-old roots. The dominant bacterial phyla Proteobacteria Firmicutes, Actinobacteria, Bacteroidetes, and Acidobacteria, and fungal phyla Ascomycota, Basidiomycota, and Ascomycota were detected in the stems and roots with 3 growth years. Moreover, linear discriminant effect size analysis revealed 138 differentially abundant taxonomic clades in the bacterial level, and 197 in the fungal level in six groups. Our results provide evidence for endophytic microbiota communities depending on the tissues and growth years of D. huoshanense. The results from this study should be useful to better understand medicinal plant-microbe interactions.

Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata

“…In this study, the endophytic fungi associated with the root, stem and leaf tissues of three-yearold H. serrata were characterized and compared. Alpha diversity analysis (Chao1, Ace, Shannon, Simpson) indicated that richness and diversity of endophytic fungi in the H. serrata root samples were lowest (Table 1), which were different from those of the D. huoshanense roots [22], that have a higher richness and diversity of endophytic fungi. A total of 555 fungal OTUs were detected from the three tissue samples of H. serrata, and were further classified into 7 phyla, 21 classes, 50 orders, 95 families and 120 genera.…”

“…Lee et al used highthroughput sequencing technology to reveal diversity of endophytic bacterial, archaeal and fungal communities inhabiting different rhizocompartments of tomato plants in real-world environments [21]. Chen et al used high-throughput sequencing technology to analyze and compare the endophytic fungal community structures associated with stems and roots of Dendrobium huoshanense [22]. However, few researches on the diversity and composition of endophytes in H. serrata based on high-throughput sequencing have been conducted.…”

Comparative Proteome and Phosphoproteome Analyses Reveal Different Molecular Mechanism Between Stone Planting Under the Forest and Greenhouse Planting of Dendrobium huoshanense

“…The wild D. huoshanense is often epiphytic to rocks and tree trunks under forest at an altitude of 200–1,200 m ( Yuan et al, 2018 ; Chen et al, 2020 ), can grow well on wet stone walls facing northwest and northeast or cliff stones with waterfalls, and often forms small communities with mosses and lichens ( Chen et al, 2020 ). Due to the unique growth environment, slow growth rates, and excessive manual collection, the wild resources of D. huoshanense are seriously threatened and cannot meet market demand ( Jin et al, 2016 ).…”

Huoshanmycins A‒C, New Polyketide Dimers Produced by Endophytic Streptomyces sp. HS-3-L-1 From Dendrobium huoshanense

“…Modern pharmacological research has revealed that D. huoshanense has anti-inflammatory ( Ge et al, 2018 ; Li et al, 2020 ), cytotoxic ( Chen et al, 2022 ), hypoglycemic ( Wang et al, 2019 ), anti-atherosclerosis ( Fan et al, 2020 ), and antioxidant ( Tian et al, 2013 ) activity. Dendrobium plant is well-known for its rich and diverse endophytic bacterial and fungal community ( Chen et al, 2019 ; Chen et al, 2020 ). Previous studies have revealed the close relationship between Dendrobium and its endophytes, such as improving the seed germination rate ( Tsavkelova et al, 2007 ) and supply of nutrients ( Li et al, 2017 ).…”

“…At present, research on D. huoshanense endophytes mainly focuses on the diversity and functions, while not much is known about their secondary metabolites, especially for bacteria. The main species of bacterial microorganisms of D. huoshanense are Sphingomonas, Acinetobacter, Enterococcus, Bacillus , and Methylobacterium ( Chen et al, 2020 ). Streptomyces is the largest genus of Actinobacteria and characterized by producing complex secondary metabolites.…”