Endophytic <i>Streptomyces</i> sp. AC35, a producer of bioactive isoflavone aglycones and antimycins

Ondrejíčková, Petra; Šturdíková, M.; Hushegyi, András; Švajdlenka, Emil; Markošová, Kristína; Čertı́k, Milan

doi:10.1007/s10295-016-1800-4

Cited by 8 publications

(5 citation statements)

References 32 publications

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“…Finally, various other compounds were tentatively identified within the extracts. The most abundant were the isoflavones daidzein [37], genistein [38] and glycitein [39] that have antioxidant, anticancer and antimicrobial properties [37,40] and several more metabolites with antibiotic properties, such as 21-demethyl-leptomycin A [41], that could explain the differences observed between the antimicrobial activity and the production levels of phocoenamicins in some of the extracts. The wide and diverse metabolic profile of these strains highlights their therapeutic potential and the need for further investigation.…”

Section: Dereplication Of the Main Compounds Producedmentioning

confidence: 99%

Exploring Micromonospora as Phocoenamicins Producers

et al. 2022

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Over the past few years, new technological and scientific advances have reinforced the field of natural product discovery. The spirotetronate class of natural products has recently grown with the discovery of phocoenamicins, natural actinomycete derived compounds that possess different antibiotic activities. Exploring the MEDINA’s strain collection, 27 actinomycete strains, including three marine-derived and 24 terrestrial strains, were identified as possible phocoenamicins producers and their taxonomic identification by 16S rDNA sequencing showed that they all belong to the Micromonospora genus. Using an OSMAC approach, all the strains were cultivated in 10 different media each, resulting in 270 fermentations, whose extracts were analyzed by LC-HRMS and subjected to High-throughput screening (HTS) against methicillin-resistant Staphylococcus aureus (MRSA), Mycobacterium tuberculosis H37Ra and Mycobacterium bovis. The combination of LC-UV-HRMS analyses, metabolomics analysis and molecular networking (GNPS) revealed that they produce several related spirotetronates not disclosed before. Variations in the culture media were identified as the most determining factor for phocoenamicin production and the best producer strains and media were established. Herein, we reported the chemically diverse production and metabolic profiling of Micromonospora sp. strains, including the known phocoenamicins and maklamicin, reported for the first time as being related to this family of compounds, as well as the bioactivity of their crude extracts. Although our findings do not confirm previous statements about phocoenamicins production only in unique marine environments, they have identified marine-derived Micromonospora species as the best producers of phocoenamicins in terms of both the abundance in their extracts of some major members of the structural class and the variety of molecular structures produced.

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Section: Dereplication Of the Main Compounds Producedmentioning

confidence: 99%

Exploring Micromonospora as Phocoenamicins Producers

et al. 2022

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“…Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins, was isolated from T. baccata rhizosphere . In the present study, we for the first time showed that the high throughput metagenomic sequencing is powerful in revealing the microbial diversity of Taxus rhizosphere.…”

Section: Discussionmentioning

confidence: 60%

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

2018

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In the present study, the shotgun high throughput metagenomic sequencing was implemented to globally capture the features of Taxus rhizosphere microbiome. Total reads could be assigned to 6925 species belonging to 113 bacteria phyla and 301 species of nine fungi phyla. For archaea and virus, 263 and 134 species were for the first time identified, respectively. More than 720,000 Unigenes were identified by clean reads assembly. The top five assigned phyla were Actinobacteria (363,941 Unigenes), Proteobacteria (182,053), Acidobacteria (44,527), Ascomycota (fungi; 18,267), and Chloroflexi (15,539). KEGG analysis predicted numerous functional genes; 7101 Unigenes belong to "Xenobiotics biodegradation and metabolism." A total of 12,040 Unigenes involved in defense mechanisms (e.g., xenobiotic metabolism) were annotated by eggNOG. Talaromyces addition could influence not only the diversity and structure of microbial communities of Taxus rhizosphere, but also the relative abundance of functional genes, including metabolic genes, antibiotic resistant genes, and genes involved in pathogen-host interaction, bacterial virulence, and bacterial secretion system. The structure and function of rhizosphere microbiome could be sensitive to non-native microbe addition, which could impact on the pollutant degradation. This study, complementary to the amplicon sequencing, more objectively reflects the native microbiome of Taxus rhizosphere and its response to environmental pressure, and lays a foundation for potential combination of phytoremediation and bioaugmentation.

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“…It was found that compounds 1 and 2 were isoflavone derivatives, which are reported for the first time in this bacterium. However, numerous isoflavone derivatives were previously identified in several Streptomyces species [ 25 , 26 , 27 , 28 ]. The identity of daidzein ( 1 ) and genistein ( 2 ) was confirmed by matching with spectroscopic data in the literature [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Priestia megaterium Metabolism: Isolation, Identification of Naringenin Analogues and Genes Elevated Associated with Nanoparticle Intervention

Al-Theyab,

Abuelizz,

Al-Hamoud

et al. 2023

CIMB

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The impact of gold nanoparticles (AuNPs) on the biosynthetic manipulation of Priestia megaterium metabolism where an existing gene cluster is enhanced to produce and enrich bioactive secondary metabolites has been studied previously. In this research, we aimed to isolate and elucidate the structure of metabolites of compounds 1 and 2 which have been analyzed previously in P. megaterium crude extract. This was achieved through a PREP-ODS C18 column with an HPLC-UV/visible detector. Then, the compounds were subjected to nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS), and Fourier-transform infrared spectroscopy (FT-IR) techniques. Furthermore, bioinformatics and transcriptome analysis were used to examine the gene expression for which the secondary metabolites produced in the presence of AuNPs showed significant enhancement in transcriptomic responses. The metabolites of compounds 1 and 2 were identified as daidzein and genistein, respectively. The real-time polymerase chain reaction (RT-PCR) technique was used to assess the expression of three genes (csoR, CHS, and yjiB) from a panel of selected genes known to be involved in the biosynthesis of the identified secondary metabolites. The expression levels of two genes (csoR and yijB) increased in response to AuNP intervention, whereas CHS was unaffected.

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Endophytic Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins

Cited by 8 publications

References 32 publications

Exploring Micromonospora as Phocoenamicins Producers

Exploring Micromonospora as Phocoenamicins Producers

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

Priestia megaterium Metabolism: Isolation, Identification of Naringenin Analogues and Genes Elevated Associated with Nanoparticle Intervention

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