Endophytic <i>Phomopsis</i> species: host range and implications for diversity estimates

Murali, Thokur Sreepathy; Suryanarayanan, T. S.; Geeta, R.

doi:10.1139/w06-020

Cited by 118 publications

(65 citation statements)

References 17 publications

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“…Similar to the present research, Murali et al (2006), working with foliar endophyte assemblages of teak trees growing in dry deciduous and moist deciduous forests of Nilgiri Biosphere Reserve, observed that the species of Phomopsis dominated the endophyte assemblages of teak. Also, in accordance with those authors, using the internal transcribed spacer sequence analysis of 11 different isolates, Phomopsis fungus is not host restricted and the species concept of Phomopsis needs to be redefined, which reinforces the previous conclusions reported by other research involving pathogenic Phomopsis species.…”

Section: G1-12supporting

confidence: 90%

Phylogenetic diversity of endophytic leaf fungus isolates from the medicinal tree Trichilia elegans (Meliaceae)

Rhoden

García²,

Filho³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Various types of organisms, mainly fungi and bacteria, live within vegetal organs and tissues, without causing damage to the plant. These microorganisms, which are called endophytes, can be useful for biological control and plant growth promotion; bioactive compounds from these organisms may have medical and pharmaceutical applications. Trichilia elegans (Meliaceae) is a native tree that grows abundantly in several regions of Brazil. Preparations using the leaves, seeds, bark, and roots of many species of the Meliaceae family have been widely used in traditional medicine, and some members of the Trichilia genus are used in Brazilian popular medicine. We assessed the diversity of endophytic fungi from two wild specimens of T. elegans, collected from a forest remnant, by sequencing ITS1-5.8S-ITS2 of rDNA of the isolates. The fungi were isolated and purified; 97 endophytic fungi were found; they were separated into 17 morpho-groups. Of the 97 endophytic fungi, four genera (Phomopsis, Diaporthe, Dothideomycete, and Cordyceps) with 11 morpho-groups were identified. Phomopsis was the most frequent genus among the identified endophytes. Phylogenetic analysis showed two major clades: Sordariomycetes, which includes three genera, Phomopsis, Diaporthe, and Cordyceps, and the clade Dothideomycetes, which was represented by the order Pleosporales.

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Section: G1-12supporting

confidence: 90%

Phylogenetic diversity of endophytic leaf fungus isolates from the medicinal tree Trichilia elegans (Meliaceae)

Rhoden

García²,

Filho³

et al. 2012

Genet. Mol. Res.

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“…Although complications resulting from ITS sequence data in Diaporthe have been recognised by several previous authors, they have not been thoroughly examined (Farr et al 2002;Murali et al 2006;Udayanga et al 2014). In Santos et al (2010) two ITS types tentatively named as A and B recovered from the isolates Di-C005/1-10 from Hydrangea in Portugal, derived from 10 individual sibling ascospores from the same perithecium were similar to the two large groups observed in our analysis ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex

et al. 2014

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The genus Diaporthe comprises pathogenic, endophytic and saprobic species with both temperate and tropical distributions. Cryptic diversification, phenotypic plasticity and extensive host associations have long complicated accurate identifications of species in this genus. The delimitation of the generic type species Diaporthe eres has been uncertain due to the lack of ex-type cultures. Species limits of D. eres and closely related species were evaluated using molecular phylogenetic analysis of eight genes including nuclear ribosomal internal transcribed spacer (ITS), partial sequences of actin (ACT), DNA-lyase (Apn2), translation elongation factor 1-α (EF1-α), beta-tubulin (TUB), calmodulin (CAL), 60s ribosomal protein L37 (FG1093) and histone-3 (HIS). The occurrence of sequence heterogeneity of ITS within D. eres is observed, which complicates the analysis and may lead to overestimation of the species diversity. The strict criteria of Genealogical Concordance Phylogenetic Species Recognition (GCPSR) were applied to resolve species boundaries based on individual and combined analyses of other seven genes except the ITS. We accept nine distinct phylogenetic species including helicis and D. pulla. Modern descriptions and illustrations are provided for these species. Newly designed primers are introduced to amplify and sequence the Apn2 (DNA-lyase) gene in Diaporthe. Based on phylogenetic informativeness profiles, EF1-α, Apn2 and HIS genes are recognised as the best markers for defining species in the D. eres complex.

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“…For example, Phomopsis fungi were widely isolated from mangroves (Suryanarayanan et al 1998; Suryanarayanan and Kumaresan 2000; Pang et al 2008; Xing et al 2011; Xing and Guo 2011) and non-mangrove plants (Taylor et al 1999; Guo et al 2000, 2008; Cannon and Simmons 2002; Murali et al 2006; Sun et al 2011, 2012). In addition, Pestalotiopsis as generalist fungi were common mangrove endophytes and widely occurred on plants across Palmae, Rhizophoraceae, Planchonellae, and Podocarpaceae in China (Liu et al 2010a, 2012; Xing and Guo 2011; Gong et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Community structure of endophytic fungi of four mangrove species in Southern China

Sun

Chen

et al. 2016

Mycology

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Mangrove forests play an important role in subtropical and tropical coastal ecosystems. Endophytic fungi are widely distributed in various ecosystems and have great contribution to global biodiversity. In order to better understand the effects of mangrove species and tissue types on endophytic fungal community, we investigated cultivable endophytic fungi in leaves and twigs of four mangroves Aegiceras corniculatum, Avicennia marina, Bruguiera gymnorrhiza, and Kandelia candel in Guangxi, China. The four tree species had similar overall colonisation rates of endophytic fungi (24–33%). The colonisation rates of endophytic fungi were higher in twigs (30–58%) than in leaves (6–25%) in the four plant species. A total of 36 endophytic fungal taxa were identified based on morphological characteristics and molecular data, including 35 Ascomycota and 1 Basidiomycota, dominated by Phomopsis, Phyllosticta, Xylaria, Leptosphaerulina, and Pestalotiopsis. The diversity of endophytic fungi was higher in twigs than in leaves in the four plant species. Some endophytic fungi showed host and tissue preference. The endophytic fungal community composition was different among four mangrove species and between leaf and twig tissues.

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Endophytic Phomopsis species: host range and implications for diversity estimates

Cited by 118 publications

References 17 publications

Phylogenetic diversity of endophytic leaf fungus isolates from the medicinal tree Trichilia elegans (Meliaceae)

Phylogenetic diversity of endophytic leaf fungus isolates from the medicinal tree Trichilia elegans (Meliaceae)

Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex

Community structure of endophytic fungi of four mangrove species in Southern China

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