Endophytic <i>Bacillus</i> spp. from medicinal plants inhibit mycelial growth of <i>Sclerotinia sclerotiorum</i> and promote plant growth

Ansary, Most. Waheda Rahman; Prince, Ferdous Rezwan Khan; Haque, Effi; Sultana, Fahmida; West, Helen; Rahman, Mahbubur; Mondol, Abdul Mojid; Akanda, Abdul Mannan; Rahman, Mukhlesur; Clarke, Michèle L.; Islam, Tofazzal

doi:10.1515/znc-2018-0002

Cited by 27 publications

(11 citation statements)

References 80 publications

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“…Past research on plant microbiomes has demonstrated growth-promoting effects ( Naik et al, 2019 ), the enhancement of plant resistance against pathogens ( Ansary et al, 2018 ; Vurukonda et al, 2018 ) and positive effects on overall plant fitness ( Rodriguez et al, 2009 ; Uroz et al, 2019 ). Further, implications of the microbiome on agricultural management ( Toju et al, 2018 ; Jones et al, 2019 ; Pacifico et al, 2019 ; Lyu et al, 2020 ), crop quality and postharvest processes ( Rillig et al, 2018 ) have been addressed.…”

Section: Introductionmentioning

confidence: 99%

The Microbiome of the Medicinal Plants Achillea millefolium L. and Hamamelis virginiana L.

et al. 2021

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In the recent past many studies investigated the microbiome of plants including several medicinal plants (MP). Microbial communities of the associated soil, rhizosphere and the above-ground organs were included, but there is still limited information on their seasonal development, and in particular simultaneous investigations of different plant organs are lacking. Many studies predominantly addressed either the prokaryotic or fungal microbiome. A distinction of epi- and endophytic communities of above-ground plant organs has rarely been made. Therefore, we conducted a comprehensive investigation of the bacterial and fungal microbiome of the MP Achillea millefolium and studied the epi- and endophytic microbial communities of leaves, flower buds and flowers between spring and summer together with the microbiome of the associated soil at one location. Further, we assessed the core microbiome of Achillea from four different locations at distances up to 250 km in southern Germany and Switzerland. In addition, the bacterial and fungal epi- and endophytic leaf microbiome of the arborescent shrub Hamamelis virginiana and the associated soil was investigated at one location. The results show a generally decreasing diversity of both microbial communities from soil to flower of Achillea. The diversity of the bacterial and fungal endophytic leaf communities of Achillea increased from April to July, whereas that of the epiphytic leaf communities decreased. In contrast, the diversity of the fungal communities of both leaf compartments and that of epiphytic bacteria of Hamamelis increased over time indicating plant-specific differences in the temporal development of microbial communities. Both MPs exhibited distinct microbial communities with plant-specific but also common taxa. The core taxa of Achillea constituted a lower fraction of the total number of taxa than of the total abundance of taxa. The results of our study provide a basis to link interactions of the microbiome with their host plant in relation to the production of bioactive compounds.

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Section: Introductionmentioning

confidence: 99%

The Microbiome of the Medicinal Plants Achillea millefolium L. and Hamamelis virginiana L.

et al. 2021

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“…Citri (Huang et al, 2012), tomato bacterial wilt caused by Ralstonia solanacearum (Chen et al, 2013), and melon bacterial fruit blotch caused by Acidovorax citrulli (Fan et al, 2017). B. subtilis strains are the dominant endophytic bacteria associated with many plant species, and they commonly exhibit the ability to enhance host plant resistance to pathogenic fungi and bacteria (Ansary et al, 2018;Kushwaha et al, 2020;Xu et al, 2019Xu et al, , 2020. These B. subtilis also do not pose a risk to humans, making them ideal for use in biocontrol applications (Earl et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Endophytic Bacillus subtilis P10 from Prunus cerasifera as a biocontrol agent against tomato Verticillium wilt

et al. 2023

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Endophytic bacteria serve key roles in the maintenance of plant health and growth. Few studies to date, however, have explored the antagonistic and plant growth-promoting (PGP) properties of Prunus cerasifera endophytes. To that end, we isolated endophytic bacteria from P. cerasifera tissue samples and used a dual culture plate assay to screen these microbes for antagonistic activity against Verticillium dahliae, Botryosphaeria dothidea, Fusarium oxysporum, F. graminearum, and F. moniliforme. Of the 36 strains of isolated bacteria, four (strains P1, P10, P16, and P20) exhibited antagonistic effects against all five model pathogens, and the P10 strain exhibited the strongest antagonistic to five pathogens. This P10 strain was then characterized in-depth via phenotypic assessments, physiological analyses, and 16s rDNA sequencing, revealing it to be a strain of Bacillus subtilis. Application of a P10 cell suspension (1×108 CFU/mL) significantly enhanced the seed germination and seedling growth of tomato in a greenhouse setting. This P10 strain further significantly suppressed tomato Verticillium wilt with much lower disease incidence and disease index scores being observed following P10 treatment relative to untreated plants in pot-based experiments. Tomato plants that had been treated with strain P10 also enhanced defense-related enzymes, peroxidase, superoxide dismutase, and catalase activity upon V. dahliae challenge relative to plants that had not been treated with this endophytic bacterium. The results revealed that the P10 bacterial strain has potential value as a biocontrol agent for use in the prevention of tomato Verticillium wilt.

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“…There are numerous studies demonstrating the suppression of Sclerotinia by Bacillus in vitro and exploring the mechanism of suppression (Li et al, 2009;Tonelli et al, 2010;Luduena et al, 2012;Figueredo et al, 2014;Vinodkumar et al, 2017;Ansary et al, 2018;Lopes et al, 2018;Massawe et al, 2018). The efficacy of Bacillus spp.…”

Section: Beneficial Bacteriamentioning

confidence: 99%

Tackling Control of a Cosmopolitan Phytopathogen: Sclerotinia

2021

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Phytopathogenic members of the Sclerotinia genus cause widespread disease across a broad range of economically important crops. In particular, Sclerotinia sclerotiorum is considered one of the most destructive and cosmopolitan of plant pathogens. Here, were review the epidemiology of the pathogen, its economic impact on agricultural production, and measures employed toward control of disease. We review the broad approaches required to tackle Sclerotinia diseases and include cultural practices, crop genetic resistance, chemical fungicides, and biological controls. We highlight the benefits and drawbacks of each approach along with recent advances within these controls and future strategies.

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Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth

Cited by 27 publications

References 80 publications

The Microbiome of the Medicinal Plants Achillea millefolium L. and Hamamelis virginiana L.

The Microbiome of the Medicinal Plants Achillea millefolium L. and Hamamelis virginiana L.

Endophytic Bacillus subtilis P10 from Prunus cerasifera as a biocontrol agent against tomato Verticillium wilt

Tackling Control of a Cosmopolitan Phytopathogen: Sclerotinia

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