Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes

Kisaakye, James; Fourie, Hendrika; Coyne, Danny; Cortada, Laura; Khamis, Fathiya M.; Subramanian, Sevgan; Masinde, Shirlyne; Haukeland, Solveig

doi:10.1163/15685411-bja10229

Cited by 6 publications

(3 citation statements)

References 36 publications

(48 reference statements)

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“…Pratylenchusgoodeyi, Radopholussimilis, P. coffeae, Helicotylenchusmulticinctus, and Meloidogyne spp. are the most nematodes responsible for many diseases in banana variety, because banana varieties are the host of root-knot nematodes [3] . Although the nematodes or root-knot nematodes have a short life period, affecting host plants they can reduce yield capacity by 20-30% as well as make a rupture in the root, which helps the secondary pathogens enter [4,5] .…”

Section: Introductionmentioning

confidence: 99%

Diversity of Endophytic Fungi in Banana Cultivars of Assam India

et al. 2023

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Endophytic fungal isolates (139 no.) were obtained from 143 (62 roots, 18 fruits and 54 leaves) samples of 15 different varieties of banana collected from 10 sites in Assam, India during 2018-2019. Overall isolation frequency from surface-sterilized tissue ranged from 10%-80% (as per site) and 6%-70% (as per variety of banana). All isolates were segregated into 40 different types on the basis of macromorphological and micro morphological characteristics. Forty different fungal taxa were isolated belonging to 14 genera including Absidia, Arthrinium, Aspergillus, Bipolaris, Cladosporium, Curvularia, Dendrophion, Fusarium, Humicola, Mortierella, Mucor, Penicillium, Paecilomyces, Verticillium and one mycelium sterile. Among them, Cladosporium cladosporioidies and Paecilomyces sp. frequently occurred in most of the sites surveyed whereas Cladospoirum cladosporioides and Aspergillus sp. 8, Fusarium graminseram were most frequently isolated from different varieties. However, all sites differed in their fungal diversity. Banana samples from Narigoan and Jorhat have been found with maximum fungal species followed by marigoan samples so as to Banana varieties Amrit Sagar endowed 27 no. of fungi followed by Jehaji and Honda which were associated with a maximum 14 fungal sp. Isolation frequency and relative abundance of Cladosporium cladosporiodes (80%, 4.6), Paecilomyces farinosus (80%, 4.6) followed by Penicillium ruburm, Aspergillus sp. 8 & 9 (70%, 4.02) were recorded as maximum comparatively in different sites. However, Aspergillus sp. 8, Mortieralla sp. and Pacilomyces farinosus are isolated frequently from different banana varieties (73.33%, 4.93).

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Section: Introductionmentioning

confidence: 99%

Diversity of Endophytic Fungi in Banana Cultivars of Assam India

et al. 2023

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“…Although they were ineffective against the nematodes, P. lilacinum promoted coffee plant growth ( Arita et al., 2020 ). In Kenya, both P. lilacinum and Trichoderma asperellum are registered for use against nematodes and are commercially available, where they have been proven effective against RKN on pineapple ( Kiriga et al., 2018 ) and T. asperellum against Radopholus similis on banana ( Kisaakye et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol-based strategies for improving soil health and managing plant-parasitic nematodes in coffee production

et al. 2023

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Coffee is an important commodity for Kenya, where production is steadily declining, despite a global rise in demand. Of the various constraints affecting production, plant-parasitic nematodes are a significant, but often overlooked, threat. As a perennial crop, treating plantations once infected with nematodes becomes difficult. The current study evaluated the drenching application of two biocontrol agents, Trichoderma asperellum and Purpureocillium lilacinum, for their nematode control efficacy, as well as their impact on the soil nematode community structure on mature, established coffee trees in Kenya. Seven Arabica coffee field trials were conducted over two years on trees of various ages. All the fields were heavily infested with Meloidogyne hapla, the first report of the species on coffee in Kenya. Both fungal biocontrol agents were detected endophytically infecting roots and recovered from soil but not until six months after initial applications. The population densities of M. hapla had significantly declined in roots of treated trees 12 months after the initial application, although soil nematode density data were similar across treatments. Based upon the maturity index and the Shannon index, treatment with T. asperellum led to improved soil health conditions and enrichment of diversity in the microbial community. Application of P. lilacinum, in particular, led to an increased abundance of fungivorous nematodes, especially Aphelenchus spp., for which P. lilacinum would appear to be a preferred food source. The soils in the trials were all stressed and denuded, however, which likely delayed the impact of such treatments or detection of any differences between treatments using indices, such as the functional metabolic footprint, over the period of study. A longer period of study would therefore likely provide a better indication of treatment benefits. The current study positively demonstrates, however, the potential for using biologically based options for the environmentally and climate-smart management of nematode threats in a sustainable manner on established, mature coffee plantations.

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“…Plant-parasitic nematodes significantly threaten banana production in tropical and subtropical regions. ____________________________________________________________________________________________________________________ Nematode infestation in bananas leads to lower bunch weight and longer crop cycles, which can significantly affect yield (Kisaakye et al 2023). Radopholus similis is a migratory endoparasitic nematode primarily found in plant roots and is widely known as a destructive pest of bananas, citrus, and black pepper.…”

Section: Introductionmentioning

confidence: 99%

Nematicidal Effect of Eupatorium odoratum Linn. Aqueous Extract on Burrowing Nematodes (Radopholus similis) and its Application to Control Toppling Disease on Cavendish Banana (Musa acuminata)

Nhung,

Quoc

2023

Journal of Horticultural Research

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Radopholus similis (Cobb 1893) (Rhabditida, Pratylenchidae) is a common and destructive pest that causes significant yield losses in Cavendish bananas. The repeated use of synthetic pesticides to control this nematode has raised concerns about toxicity to users and the environment. An alternative approach to managing this harmful species involves the use of nontoxic plant-derived extracts that enhance protection against various biological constraints. In this study, aqueous extract from the leaves and stems of Eupatorium odoratum Linn. (EAEO) was used to control R. similis in banana roots under in vitro and on-plant conditions. Through direct contact and penetration experiments on roots, as well as on-plant trials, concentrations of 12500, 6250, 3120, 1560, and 780 mg DW·L−1 EAEO were used to evaluate nematode mortality rates, infection levels in root segments (in vitro experiment), root infection, root necrosis index, shoot and root fresh weight, and growth parameters such as stem length, diameter, leaf number, and leaf surface area (on-plant experiment). The results showed that the nematode mortality rate due to EAEO ranged from 35.3% to 90.1%, which is statistically significant compared to the mortality rate in the control treatment (16%). Significant nematode inhibition was observed in banana roots infected with R. similis and exposed to EAEO for 48 h. There were no statistically significant differences in root infection, root necrosis, and plant fresh weight between plants treated with fenamiphos and EAEO. Maximum effectiveness was observed at the 12500 mg DW·L−1 EAEO concentration. These findings provide valuable insights into the nematicidal metabolites of EAEO against R. similis. Thus, the potential of EAEO as a biocontrol agent against R. similis has been demonstrated. For future research, investigations will be extended to elucidate the mechanisms of action of the essential compounds in EAEO against nematodes, determine the optimal EAEO dosage for nematode control without harm to banana plants and the environment, assess the long-term effects of EAEO on banana plants and the environment, explore the ecological impact of EAEO use in agricultural ecosystems, study the effectiveness of EAEO on various plant species, and consider the development of commercial products or formulations based on EAEO.

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Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes

Cited by 6 publications

References 36 publications

Diversity of Endophytic Fungi in Banana Cultivars of Assam India

Diversity of Endophytic Fungi in Banana Cultivars of Assam India

Biocontrol-based strategies for improving soil health and managing plant-parasitic nematodes in coffee production

Nematicidal Effect of Eupatorium odoratum Linn. Aqueous Extract on Burrowing Nematodes (Radopholus similis) and its Application to Control Toppling Disease on Cavendish Banana (Musa acuminata)

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