Endophytic fungi from Dongxiang wild rice (Oryza rufipogon Griff.) show diverse catalytic potential for converting glycyrrhizin

Yang, Xiao; Zhang, Zhibin; Liang, Weizhong; Gao, Boliang; Ya, Wang; Chang, Jun; Zhu, Du

doi:10.1007/s13205-022-03138-x

Cited by 8 publications

(10 citation statements)

References 41 publications

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“…Recently, some microorganism, mainly yeast, have been employed to transform mogrosides due to their glucosidase activity, showing the ability for converting mogroside V into a mixture of mogroside IV, siamenoside I, and mogroside III E (Chiu et al 2013 ; Wang et al 2019 ). According to our previous studies, fungal endophytes secreted rich glycosidases and exhibited the ability to transform terpenoid saponins, such as glycyrrhizic acid (Xiao et al 2022 ). Therefore, we supposed that the fungal endophytes maybe have the capacity to transform mogrosides.…”

Section: Resultsmentioning

confidence: 99%

“…Especially, these enzymes secreted, which are used for plant tissue invasion, colonization, and nutrient acquisition by endophytic fungi, make them a valuable source of biocatalysts for the biotransformation of natural products. (Higginbotham et al 2013 ; Suryanarayanan et al 2012 ; Gao et al 2021 ; Xiao et al 2022 ). Recently, some studies have found that plant fungal endophytes exhibit excellent properties in bioconversion of natural saponin, such as glycyrrhizic acid (Gao et al 2021 ; Xiao et al 2022 ), ginsenosides (Eom et al 2018 ), with high activity and substrate specificity.…”

Section: Introductionmentioning

confidence: 99%

“…(Higginbotham et al 2013 ; Suryanarayanan et al 2012 ; Gao et al 2021 ; Xiao et al 2022 ). Recently, some studies have found that plant fungal endophytes exhibit excellent properties in bioconversion of natural saponin, such as glycyrrhizic acid (Gao et al 2021 ; Xiao et al 2022 ), ginsenosides (Eom et al 2018 ), with high activity and substrate specificity. Indeed, fungal endophytes have been suggested as high-efficient biocatalyst sources, revealing great potential application in medicine, food security, and social sustainability (Schulz et al 2002 ; Suryanarayanan et al 2012 ; Choudhary et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Plant endophytic fungi exhibit diverse biotransformation pathways of mogrosides and show great potential application in siamenoside I production

Lin,

Jiang,

Dong

et al. 2024

Bioresour. Bioprocess.

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Fungal endophytes, as an untapped resource of glycoside hydrolase biocatalysts, need to be further developed. Mogroside V, the primary active compound in Siraitia grosvenorii fruit, can be converted into other various bioactive mogrosides by selective hydrolysis of glucose residues at C3 and C24 positions. In present study, 20 fungal strains were randomly selected from our endophytic fungal strain library to assess their capability for mogroside V transformation. The results revealed that relatively high rate (30%) endophytic fungal strains exhibited transformative potential. Further analysis indicated that endophytic fungi could produce abundant mogrosides, and the pathways for biotransforming mogroside V showed diverse. Among the given fungal endophytes, Aspergillus sp. S125 almost completely converted mogroside V into the end-products mogroside II A and aglycone within just 2 days of fermentation; Muyocopron sp. A5 produced rich intermediate products, including siamenoside I, and the end-product mogroside II E. Subsequently, we optimized the fermentation conditions for Aspergillus sp. S125 and Muyocopron sp. A5 to evaluate the feasibility of large-scale mogroside V conversion. After optimization, Aspergillus sp. S125 converted 10 g/L of mogroside V into 4.5 g/L of mogroside II A and 3.6 g/L of aglycone after 3 days of fermentation, whereas Muyocopron sp. A5 selectively produced 4.88 g/L of siamenoside I from 7.5 g/L of mogroside V after 36 h of fermentation. This study not only identifies highly effective biocatalytic candidates for mogrosides transformation, but also strongly suggests the potential of plant endophytic fungi as valuable resources for the biocatalysis of natural compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plant endophytic fungi exhibit diverse biotransformation pathways of mogrosides and show great potential application in siamenoside I production

Lin,

Jiang,

Dong

et al. 2024

Bioresour. Bioprocess.

Self Cite

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“…Endophytic fungi are an ecological group of fungi that inhabit the tissues and organs of healthy plants, without causing any external symptoms of the host plant (Staniek et al, 2008). In last decades, endophytic fungi have been attracted more and more attention due to its excellent capacity for secreting a diverse array of bioactive compounds and enzymes (Higginbotham et al, 2013;Suryanarayanan et al, 2012;Gao et al, 2021;Xiao et al, 2022). Recently, some studies have found that plant fungal endophytes exhibit excellent properties in bioconversion of natural saponin, such as glycyrrhizic acid (Gao et al, 2021;Xiao et al, 2022), ginsenosides (Eom et al, 2018), with high activity and substrate speci city.…”

Section: Introductionmentioning

confidence: 99%

Plant endophytic fungi exhibit diverse biotransformation pathways of mogrosides and show great potential application in siamenoside Ⅰ production

Lin,

Jiang,

Dong

et al. 2024

Preprint

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Fungal endophytes, as an untapped resource of glycoside hydrolase biocatalysts, need to be further developed. The primary active compound in the fruit of Momordica grosvenorii, mogroside V, can be converted into other various bioactive mogrosides by selective hydrolysis of glucose residues at C3 and C24 positions. In present study, 20 fungal strains were randomly selected from our endophytic fungal strain library to investigate their capability for transforming mogroside V. The results revealed that relatively high rate (30%) endophytic fungal strains exhibited the ability of transformation. Further analysis indicated that endophytic fungi could produce abundant mogrosides, and the pathways for biotransforming mogroside V showed diverse. Among the given fungal endophytes, Aspergillus sp. S125 could almost completely transform mogroside V into the end-products mogroside II A and aglycone only after 2 days of fermentation; Muyocopron sp. A5 produced rich intermediate products, including siamenoside Ⅰ, and the end-product mogroside II E. Furthermore, Aspergillus sp. S125 and Muyocopron sp. A5 were selected to optimize the fermentation conditions in order to evaluate the feasibility of large-scale conversion of mogroside V. After optimization, Aspergillus sp. S125 could convert 10 g/L of mogroside V into 4.5 g/L of mogroside II A and 3.6 g/L of aglycone after 3 days of fermentation, while Muyocopron sp. A5 could selectively produce 4.88 g/L of siamenoside Ⅰ from 7.5 g/L of mogroside V after 36 hours of fermentation. This study not only provides a class of highly effective biocatalytic candidates for transform mogrosides, but also strongly indicates that plant endophytic fungi can be used as a potential resource for biocatalysis of natural compounds.

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“…These core microorganisms could be applied as potential biological inoculants in crops to boost sustainable production [21]. Endophytic fungi, bene cial symbiotes with high abundance in wild rice [22][23][24], offer a variety of mild bene ts to their hosts, acting as biological control agents for rice growth and diseases [25,26]. Therefore, it is of tremendous scienti c value to dig endophytic fungi resources in wild rice and apply them to cultivated crop production.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Acrocalymma vagum drives the microbiome variations for rice health

Zeng

Wang

et al. 2023

Preprint

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Background: Endophytic fungi are essential members of the plant microbial community, which play a vital role in promoting plant growth and development, and inducing disease resistance. However, little is known about the ecological mechanisms of endophytic fungi impacting the root microbiota community to improve crop health. Results: An endophytic fungus Acrocalymma vagum (Code A21) isolated from wild rice enhanced rice yield by 5.73% and induced resistance against rice blast with 83.24% control efficiency in the field. The root microbiota community variations were discovered through 16S and ITS metabarcoding methods. A. vagum simplified the complexity of the rice microbiome community, reducing the proportion of the dominant pathogens Burkholderia and Ralstonia, and driving rice to recruit beneficial bacteria (Aquabacterium, Flavobacterium, and Lactobacillus) and fungi (Rhizophagus, Sarocladium, and Nigrospora). The recruited microbes were enriched in nutrient synthesis and stress resistance, especially biosynthesis of lactate, vitamin B12, and thiazole. We further isolated and identified 279 bacteria and 82 fungi from the A. vagum-fertilized rice roots to confirm the probiotic's functions. Among them, 5 Lactobacillus strains and 2 Nigrospora strains possessed significantly inhibitive activity on rice blast pathogen Magnaporthe oryzae. Additionally, 3 fungal strains in Sarocladium and Nigrospora genera promoted rice growth. Mutualistic mechanisms of A. vagum-rice were uncovered by the LC-MS/MS-based label-free quantitative assay. 13 secretory proteins of A. vagum cooperated to evade the host immune response and stimulate the symbiont resistance, such as alkaline phosphatases (ALP) as specific marker enzymes to symbiosis with the host and glycoside hydrolases (GHs) as effectors to colonize the host. Correspondingly, 206 rice proteins were induced to up-regulate, especially families of peroxidase (OsPRX), copper transporter (OsCOPT), and glutathione S-transferase (OsGSTU) related to growth and stress resistance. Conclusion: Our study verified that endophytic fungi isolated from wild rice had great practical value on cultivated rice. A. vagum reshaped the microbiota community of cultivated rice by enhancing host traits, as the first report of simplification and optimization effect of endophytic fungi on plant recruiting microbes, which offers novel perspectives on using endophytic fungi to increase agricultural productivity.

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Endophytic fungi from Dongxiang wild rice (Oryza rufipogon Griff.) show diverse catalytic potential for converting glycyrrhizin

Cited by 8 publications

References 41 publications

Plant endophytic fungi exhibit diverse biotransformation pathways of mogrosides and show great potential application in siamenoside I production

Plant endophytic fungi exhibit diverse biotransformation pathways of mogrosides and show great potential application in siamenoside I production

Plant endophytic fungi exhibit diverse biotransformation pathways of mogrosides and show great potential application in siamenoside Ⅰ production

Endophytic Acrocalymma vagum drives the microbiome variations for rice health

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