Endophytic community of Pb-Zn hyperaccumulator Arabis alpina and its role in host plants metal tolerance

Sharma, Vijay Kumar; Li, Xinya; Guangli, WU; Bai, Weixiao; Parmar, Shobhika; White, James F.; Li, Haiyan

doi:10.1007/s11104-019-03988-0

Cited by 24 publications

(13 citation statements)

References 51 publications

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“…In the scientific literature, we did not find C. magnusianum as a partner influencing the resistance of plants to heavy metals. However, there are many publications confirming the role, for example, of arbuscular mycorrhizal fungi in the formation of plant resistance to heavy metals (Seguel et al, 2013;Shabani et al, 2016;Spagnoletti et al, 2016;Sharma et al, 2019). The results of our research, using non-biogenic chemical elements that are hazardous to plant life, are similar.…”

Section: Resultssupporting

confidence: 75%

“…In general, this heterogeneous group of fungi can have a strong effect on plant communities by ensuring the resistance of plants to abiotic and biotic stress. Studies on the endophytes' role in the formation of plants' resistance to metals, including crops (Rodriguez et al, 2009;Ikram et al, 2018;Bilal et al, 2019;Dabral et al, 2019;El-Samad et al, 2019), and with regard to chemical elements that are extremely dangerous for plants (Ali et al 2019;Bilal et al, 2020;Li et al, 2019;Sharma et al, 2019;Hou et al, 2020) are of particular interest. A number of studies are aimed at examining the possibility of using micromycetes as herbicides (Sogonov & Velikanov, 2004;Boyette et al, 2016;Boyette et al, 2018;Meepagala et al, 2019;Sobowale, 2019).…”

Section: Introductionmentioning

confidence: 99%

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The effect of inoculation of the root system with Cylindrocarpon magnusianum on plant performance exposed to heavy metal salts

Bukharina

Islamova

2021

Agraria

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The effect of inoculation with endotrophic micromycete Cylindrocarpon magnusianum on the formation of adaptive physiological and biochemical reactions in test tomato plants under the action of heavy metal salts has been studied. The experimental design included plant inoculation with a fungus culture (control population) and these fungus populations were preliminarily grown on a pentose-dextrose agar medium with the addition of heavy metal salts in different concentrations. The inoculated plants were grown on substrates without the addition of heavy metal salts (control) and with the addition of zinc, copper, lead, and chromium salts in different concentrations similar to additions in agar when growing fungus populations. In experimental plants, we determined the content of photosynthetic pigments and ascorbic acid in the leaves (spectrophotometry), nitrates in the leaves (ionometric analysis), the aerial part and root system biomass by the gravimetric method, and the degree of fungal infection development of plants via the light microscopy method of macerated and colored fragments of the plant root system. No stimulatory effect, increasing the resistance of plants to the action of heavy metal salts when plants were inoculated with a control population of the C. magnusianum fungus, was revealed. A positive effect was revealed during the inoculation of plants with fungal populations, previously adapted to the action of salts of chemical elements, especially chromium and lead salts, and during further plants' cultivation on substrates with the introduction of these non-biogenic chemical elements hazardous to the life of plants. The effect is associated with the stability of the pigment system, the redistribution of plant biomass, and the synthesis of the antioxidant-ascorbic acid in the leaves. Intensive development of fungal infection in plant roots was also noted. The study results indicate the most effective partnership between the C. magnusianum fungus and the plant root system under stressful conditions for plant life.

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Section: Resultssupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

The effect of inoculation of the root system with Cylindrocarpon magnusianum on plant performance exposed to heavy metal salts

Bukharina

Islamova

2021

Agraria

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“…Compared to the aquatic lifestyle of Tetracladium species (2), little is known about the biology or ecological importance of Tetracladium living outside of water. In vitro , some species are known to degrade lignin (39), pectin (40), startch (41), solubilize phosphate (42), have antimicrobial activity (43, 44), and to increase metal tolerance in host plants (45). Correlation studies have associated Tetracladium with increased plant growth (46), however, clear patterns are not yet emerging from which the roles or impact of Tetracladium as endophytes or in soil can be inferred (47).…”

Section: Introductionmentioning

confidence: 99%

Broad geographical and ecological diversity from similar genomic toolkits in the ascomycete genusTetracladium

Anderson

Marvanová

2020

Preprint

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18The ascomycete genus Tetracladium is best known for containing aquatic hyphomycetes, which are 19 important decomposers in stream food webs. However, some species of Tetracladium are thought 20 to be multifunctional and are also endobionts in plants. Suprisingly, Tetracladium sequences are 21 increasingly being reported from metagenomics and metabarcoding studies of both plants and soils 22 world-wide. It is not clear how these sequences are related to the described species and little is 23 known about the non-aquatic biology of these fungi. Here, the genomes of 24 Tetracladium strains, 24 including all described species, were sequenced and used to resolve relationships among taxa and to 25 improve our understanding of ecological and genomic diversity in this group. All genome-sequenced 26 Tetracladium fungi form a monophyletic group. Conspecific strains of T. furcatum from both aquatic 27 saprotrophic and endobiont lifestyles and a putative cold-adapted clade are identified. Analysis of 28 ITS sequences from water, soil, and plants from around the world reveals that multifunctionality 29 may be widespread through the genus. Further, frequent reports of these fungi from extreme 30 environments suggest they may have important but unknown roles in those ecosystems. Patterns of 31 predicted carbohydrate active enzymes (CAZyme) and secondary metabolites in the Tetracladium 32 genomes are more similar to each other than to other ascomycetes, regardless of ecology, 33 suggesting a strong role for phylogeny shaping genome content in the genus. Tetracladium genomes 34 are enriched for pectate lyase domains (including PL3-2), GH71 α-1,3-glucanase domains and CBM24 35 α-1,3-glucan/mutan binding modules, and both GH32 and CBM38, inulinase and inulin binding 36 modules. These results indicate that these fungi are well-suited to digesting pectate and pectin in 37 leaves when living as aquatic hyphomycetes, and inulin when living as root endobionts. Enrichment 38 for α-1,3-glucanase domains may be associated with interactions with biofilm forming 39 microorganisms in root and submerged leaf environments. 42 important decomposers in stream ecosystems. The group is polyphyletic, its members are classified 43 in various orders and families of Ascomycetes and Basidiomycetes. These fungi thrive on leaves and 44 other plant debris that enter streams and other water bodies from terrestrial plants. In the process, 45 carbon and nutrients from recalcitrant plant compounds, including cellulose and lignin, become 46 accessible to diverse consumers in the stream food web (reviewed in (1, 2)). Up to 99% of the carbon 47 in some streams enters as plant debris that is largely inaccessible to aquatic consumers without the 48 degradative activity of these fungi (3, 4). Aquatic hyphomycete adaptations to stream environments 49 include the production of asexual propagules (conidia) while under water. These spores are often 50 branched, typically tetraradiate, or sigmoid, readily detachable from the fungus (e.g. from 51 conidiophores) and passi...

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“…Several studies reported the ability of hyperaccumulators to recruit bene cial microbes to enhance growth and tolerate environmental stress, including heavy metal stress (Miransari 2011). As a consequence, combining the use of hyperaccumulators and bene cial microorganisms to promote the phytoremediation processes appeared as a promising alternative biotechnology (Gupta and Joia 2016).This approach develops the association of assistant endophytes, rhizospheric bacteria and/or fungi to remove metals from contaminated soils through enhancing plant biomass production and facilitating phytoextraction or reducing phytostabilization (Glick 2010), (Ma et al 2016), (Sharma et al 2019). As an example, Arabis alpina was shown to host highly complex fungal and bacterial communities that actively contribute to increased heavy metal tolerance (Sharma et al 2019), (Sun et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…As a consequence, combining the use of hyperaccumulators and bene cial microorganisms to promote the phytoremediation processes appeared as a promising alternative biotechnology (Gupta and Joia 2016).This approach develops the association of assistant endophytes, rhizospheric bacteria and/or fungi to remove metals from contaminated soils through enhancing plant biomass production and facilitating phytoextraction or reducing phytostabilization (Glick 2010), (Ma et al 2016), (Sharma et al 2019). As an example, Arabis alpina was shown to host highly complex fungal and bacterial communities that actively contribute to increased heavy metal tolerance (Sharma et al 2019), (Sun et al 2019). More generally, dark septate endophytes (DSEs), a diverse group among ascomycetes (Jumpponen and Trappe 1998), and arbuscular mycorrhizal fungi (AMFs), which belong to the phylum Glomeromycota among Mucoromycetes (Brundrett and Tedersoo 2018), establish endophytic relationships with their hosts which result in improved extraction properties of the plant (Pawlowska et al 2000;Torrecillas et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Intercropping-induced shifts in root microbiota promote lead phytoremediation properties of Sonchus Asper

X¹,

Y²,

Z³

et al. 2021

Preprint

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Intercropping or assistant-endophytes has been proven to promote phytoremediation capacities of hyperaccumulators and to enhance their tolerance to heavy metals (HM) stress. We initially showed that intercropping with maize improved the remediating properties of the hyperaccumulator Sonchus asper (L.) Hill grown in HM contaminated soils, accompanied by an excluder - to- hyperaccumulator switched mode of action towards lead. To characterize molecular events underlying this shift in lead extraction strategy, we conducted an RNA-Seq analysis on Sonchus roots grown in intercropping or monoculture. We show that intercropping only slightly affects the S. asper transcriptome, but dramatically impacts the expression of root associated microbial genomes. Further, intercropping triggers a severe reshaping of endophytic communities which accompanies a “root-to-shoot” transition of lead sequestration and improved phytoremediation capacities of S. asper. This study provides the clue that a unitive network of plant- microbial -plant interactions may participate to the phytoremediation abilities of hyperaccumulator plants and paves the path for innovative cultural practices aiming to cure polluted soils.

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Endophytic community of Pb-Zn hyperaccumulator Arabis alpina and its role in host plants metal tolerance

Cited by 24 publications

References 51 publications

The effect of inoculation of the root system with Cylindrocarpon magnusianum on plant performance exposed to heavy metal salts

The effect of inoculation of the root system with Cylindrocarpon magnusianum on plant performance exposed to heavy metal salts

Broad geographical and ecological diversity from similar genomic toolkits in the ascomycete genusTetracladium

Intercropping-induced shifts in root microbiota promote lead phytoremediation properties of Sonchus Asper

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