Endophytic bacterial flora in root and stem tissues of black pepper (Piper nigrumL.) genotype: isolation, identification and evaluation againstPhytophthora capsici

Abstract

Aim: To isolate and identify black pepper (Piper nigrum L) associated endophytic bacteria antagonistic to Phytophthora capsici causing foot rot disease. Methods and Results: Endophytic bacteria (74) were isolated, characterized and evaluated against P. capsici. Six genera belong to Pseudomonas spp (20 strains), Serratia (1 strain), Bacillus spp. (22 strains), Arthrobacter spp. (15 strains), Micrococcus spp. (7 strains), Curtobacterium sp. (1 strain) and eight unidentified strains were isolated from internal tissues of root and stem. Three isolates, IISRBP 35, IISRBP 25 and IISRBP 17 were found effective for Phytophthora suppression in multilevel screening assays which recorded over 70% disease suppression in green house trials. A species closest match (99% similarity) of IISRBP 35 was established as Pseudomonas aeruginosa (Pseudomonas EF568931), IISRBP 25 as P. putida (Pseudomonas EF568932), and IISRBP 17 as Bacillus megaterium (B. megaterium EU071712) based on 16S rDNA sequencing. Conclusion: Black pepper associated P. aeruginosa, P. putida and B. megaterium were identified as effective antagonistic endophytes for biological control of Phytophthora foot rot in black pepper. Significance and Impact of the Study: This work provides the first evidence for endophytic bacterial diversity in black pepper stem and roots, with biocontrol potential against P. capsici infection.

Identification and determination of characteristics of endophytes from rice plants and their role in biocontrol of bacterial blight caused by Xanthomonas oryzae pv. oryzae

“…al., 2007Mano and Morisaki, 2008;Cottyn et al, 2009;Kaga et al, 2009;Ji et al, 2014;Nhu and Diep, 2014 (Barka et al, 2002;Oliveira et al, 2003;Kang et al, 2007;Szilagyi-Zecchin et al, 2014). In addition, endophytic bacteria have been reported to be involved with other mechanisms such as competition for place and food, and antibiosis (Sturz et al, 2000;Reiter et al, 2002;Compant et al, 2005;Aravind et al, 2009;Ji et al, 2013). Especially in rice, Ji et al (2013) found that treatment of rice seeds with the diazotrophic endophytes Paenibacillus, Microbacterium, Bacillus and Klebsiella, induced systemic resistance against Fusarium oxysporum and Rhizoctonia solani in addition to the growth induction of the rice plant.…”

Endophytic bacteria from Piper tuberculatum Jacq.: isolation, molecular characterization, and in vitro screening for the control of Fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (Piper nigrum L.)

“…Pseudomonas is a well-known plant-associated bacterium found in the majority of plant species. These bacteria have been effective in the biological control of diverse diseases, such as foot rot in black pepper (Aravind et al, 2009) and grapevine gray mold caused by Botrytis cinerea (Verhagen et al, 2010). Additionally, Pseudomonas bacteria are considered plant growth-promoting bacteria (PGPB) due to many traits, which contribute to the growth and development of plants, such as the production of phytohormones and siderophores (Glick, 2012).…”

“…Antagonistic bacteria for the biological control of nursery wilt in black pepper were isolated by Anith et al (2003). In addition, Aravind et al (2009) isolated Bacillus and Pseudomonas bacteria that are effective in the biological control of Phytophthora capsici, the causal agent of the black pepper foot rot disease in India and other Asian countries.…”

Mitigating Phytophthora foot rot and slow decline diseases of black pepper through the deployment of bacterial antagonists

“…Each 1g sample was added to 0.2% sterile water agar (90 mL), stirred using magnetic stirrer for 5 min, and serially diluted and the dilutions of 10 -6 and 10 -8 were plated in nutrient agar (NA) for bacterial counts and dilutions of 10 -3 and 10 -5 in Rose Bengal agar (RBA) for fungal counts. For enumerating the introduced bacteria, NA amended with selective antibiotics and TTC were used (Aravind et al 2008).…”