Endophytic Bacterial Communities Associated with Roots and Leaves of Plants Growing in Chilean Extreme Environments

Zhang, Qian; Acuña, Jacquelinne J.; Inostroza, Nitza G.; Mora, Marı́a de la Luz; Radic, Sergio; Sadowsky, Michael J.; Jorquera, Milko A.

doi:10.1038/s41598-019-41160-x

Cited by 77 publications

(60 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other families such as Nocardioidaceae, Sphingomonadaceae, Streptomycetaceae, were found in both plants, with different relative abundances. Nocardioidaceae and Streptomycetaceae families have been previously reported in rhizosphere [74,79] and bulk soil [75] in native plants of the Atacama Desert Altiplano. With respect to the family Sphingomonadaceae different strains of Sphingomonas spp.…”

Section: Discussionmentioning

confidence: 93%

“…also native of the Atacama Desert [72] and in other arid ecosystems, Tribulus terrestris, Zygophyllum simplex, Panicum turgidum, and Euphorbia granulata of Saudi Arabia Desert [73]. Also, recent studies of endophytic bacterial communities in Distichilis spicata and Pluschea absinthioides have identified Proteobacteria as a higher dominant phylum [74].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

et al. 2020

View full text Add to dashboard Cite

The rhizosphere microbiome is key in survival, development, and stress tolerance in plants. Salinity, drought, and extreme temperatures are frequent events in the Atacama Desert, considered the driest in the world. However, little information of the rhizosphere microbiome and its possible contribution to the adaptation and tolerance of plants that inhabit the desert is available. We used a high-throughput Illumina MiSeq sequencing approach to explore the composition, diversity, and functions of fungal and bacterial communities of the rhizosphere of Baccharis scandens and Solanum chilense native plants from the Atacama Desert. Our results showed that the fungal phyla Ascomycota and Basidiomycota and the bacterial phyla Actinobacteria and Proteobacteria were the dominant taxa in the rhizosphere of both plants. The linear discriminant analysis (LDA) effect size (LefSe) of the rhizosphere communities associated with B. scandens showed the genera Penicillium and Arthrobacter were the preferential taxa, whereas the genera Oidiodendron and Nitrospirae was the preferential taxa in S. chilense. Both plant showed similar diversity, richness, and abundance according to Shannon index, observed OTUs, and evenness. Our results indicate that there are no significant differences (p = 0.1) between the fungal and bacterial communities of both plants, however through LefSe, we find taxa associated with each plant species and the PCoA shows a separation between the samples of each species. This study provides knowledge to relate the assembly of the microbiome to the adaptability to drought stress in desert plants.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Four major phyla detected in all tested Bt-chickpea tissues are Proteobacteria (0.35), Cyanobacteria (0.35), Actinobacteria (0.17), and Firmicutes (0.07). Similarly, Zhang et al reported a greater relative abundance of Proteobacteria (14% to 68%), Firmicutes (26% to 41%), Actinobacteria (6 to 23%) and Bacteroidetes (1% to 21%) in plants struggling for existence in the extreme environments 38 .…”

Section: Discussionmentioning

confidence: 89%

“…Endophytic colonization by bacteria is ubiquitous in nature and plants deprived of endophytes would be more vulnerable to biotic and abiotic stresses [34][35][36][37] . Endophytic bacterial isolates, belonging to Enterobacteria, Pseudomonas, and Bacillus were established in several surrogate hosts and improve their growth and yield 38 . In addition, they conferred stress tolerance to agricultural crops against drought, flood, heavy metal toxicity, and salinity by improving antioxidant defense mechanisms, maintaining ROS levels, producing ACC deaminase, altering the selectivity of K+, Na+ and Ca2+ and maintaining higher K+/Na+ ratio 39 .…”

Section: Discussionmentioning

confidence: 99%

Symbiotic nitrogen fixation and endophytic bacterial community structure in Bt-transgenic chickpea (Cicer arietinum L)

Das

Annapragada

Singh

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Symbiotic nitrogen fixation (SNF) of transgenic grain legumes might be influenced either by the site of transgene integration into the host genome or due to constitutive expression of transgenes and antibiotic-resistant marker genes. The present investigation confirmed proper nodulation of five tested Bt-chickpea events (IPCa2, IPCa4, IPCT3, IPCT10, and IPCT13) by native Mesorhizobium under field environment. Quantitative variations for nodulation traits among Bt-chickpea were determined and IPCT3 was found superior for nodule number and nodule biomass. Diversity, as well as richness indices, confirmed the changes in bacterial community structure of root and root-nodules from Bt-chickpea events IPCa2 and IPCT10. Especially, Gram-positive bacteria belonging to Firmicutes and Actinobacteria were selectively eliminated from root colonization of IPCa2. Richness indices (CHAO1 and ACE) of the root-associated bacterial community of IPCa2 was 13-14 times lesser than that of parent cv DCP92-3. Root nodule associated bacterial community of IPCT10 was unique with high diversity and richness, similar to the roots of non-Bt and Bt-chickpea. It indicated that the root nodules of IPCT10 might have lost their peculiar characteristics and recorded poor colonization of Mesorhizobium with a low relative abundance of 0.27. The impact of Bt-transgene on bacterial community structure and nodulation traits should be analyzed across the years and locations to understand and stabilize symbiotic efficiency for ecosystem sustainability.

show abstract

“…In fact, the microbiome is so important to plant existence that the microorganisms within plants may describe as much of the phenotypic variation, as the plant genotype [13,14]. The complex endophytic microbial communities, which colonize inside the the rice plants, perform a significant role in plant growth and health [53]. The various studies have established that endophytic bacterial communities change with intraspecific genetic differences and age of host.…”

Section: Introductionmentioning

confidence: 99%

Endosphere microbiome assemblage in scented black rice is affected by plant development, with possible functional roles

Singha

Singh

Maheswari

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Endophytic bacterial community of plant helps in plant growth and health. However, compositional and functional responses of bacterial endophyte communities in black rice and its correlation with antioxidant property are still not understood. Black scented rice, Chakhao ( Oryza sativa L.) is important for its unique fragrance, high antioxidant and anthocyanin content. Here, the compositional and functional role of the endophytic bacterial community, associated with black scented rice - Chakhao ( Oryza sativa L.) at young and mature stage of plant growth, in correlation with antioxidant property has been elucidated.Result: Bray–Curtis community dissimilarity analysis confirmed overlapping of community in shoot and root tissues at the young stage, but not in mature plants. Proteobacteria was found to be the most dominant phyla, and along with Cyanobacteria and Planctomycetes, it dominated the core endospheric microbiome. The genera Agrobacterium, Pleomorphomonas, Bradyrhizobium, Novasphingobium, Caulobacter, Devosia were the most abundant. The antioxidant activity of mature stage plants was found to be higher in comparison to young plants. The total polyphenol content (TPC) was found to be highest in root of Sempak variety (89.06 µg GAE/g). The relative abundance of Pleomorphomonas was positively correlated with TPC, while Gemmata , Unclassified Pirellulaceae , Unclassified Stramenopiles positively correlated with total flavonoid content (TFC). Accordingly, functional metagenome analysis of the endophytic microbiome revealed that few unique genes (naringenin-3-dioxygenase and anthocyanidin-3-O-glucosyltransferases) for flavonoid and anthocyanin synthesis were abundant in mature stage of plant development. Specific enrichment of the antioxidant producing genes in the mature plant endophytic microbiome was assigned to bacteria such as Streptomyces, Pantoea and Bradyrhizobium , which might have contributed to the common pathway of flavonoid synthesis. Conclusion: This study allows us to recognize the linkage between the endophytic bacterial community dynamics and antioxidant activity of scented black rice plant and its comparative account at young and mature stages of growth.

show abstract

Endophytic Bacterial Communities Associated with Roots and Leaves of Plants Growing in Chilean Extreme Environments

Cited by 77 publications

References 58 publications

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

Symbiotic nitrogen fixation and endophytic bacterial community structure in Bt-transgenic chickpea (Cicer arietinum L)

Endosphere microbiome assemblage in scented black rice is affected by plant development, with possible functional roles

Contact Info

Product

Resources

About