Endophytic bacteria inCoffea arabica L.

Abstract

Eighty-seven culturable endophytic bacterial isolates in 19 genera were obtained from coffee plants collected in Colombia (n = 67), Hawaii (n = 17), and Mexico (n = 3). Both Gram positive and Gram negative bacteria were isolated, with a greater percentage (68%) being Gram negative. Tissues yielding bacterial endophytes included adult plant leaves, various parts of the berry (e.g., crown, pulp, peduncle and seed), and leaves, stems, and roots of seedlings. Some of the bacteria also occurred as epiphytes. The highest number of bacteria among the berry tissues sampled was isolated from the seed, and includes Bacillus , Burkholderia , Clavibacter , Curtobacterium , Escherichia , Micrococcus , Pantoea , Pseudomonas , Serratia , and Stenotrophomonas . This is the first survey of the endophytic bacteria diversity in various coffee tissues, and the first study reporting endophytic bacteria in coffee seeds. The possible role for these bacteria in the biology of the coffee plant remains unknown.

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

“…However, the frequency of endophytic bacteria has been shown to be lower than isolation of fungi, which could potentially be explained because the conditions of rainfall and temperature were not favorable to obtain maximal bacterial colonization. Similarly, Vega et al (2005) reported that no difference in bacterial colonization was observed between the different parts of plants, which was confirmed by Arnold and Herre (2003) who discussed that rain, humidity, and temperature can influence the occurrence of endophytes.…”

“…Bacillus is a Gram-positive, spore-forming, rod-shaped genus with high tolerance to adverse ecological conditions. Previous studies have shown that endophytic bacteria of the genus Bacillus are present in: Coffea arabica L. (Vega et al, 2005), sunflower (Forchetti et al, 2007), cotton (Misaghi and Donndelinger, 1990), potato (Sessitsch et al, 2004), strawberry (Dias et al, 2008), Panaxnoto ginseng (Ma et al, 2013), and citrus plants (Araujo et al, 2001). Among the bioprospection factors, the endophyte Bacillus stands out for phosphate solubilization and auxin production (Dias et al, 2008) and activity against Fusarium oxysporum, Ralstonia sp, and Meloidogyne hapla (Ma et al, 2013).…”

“…The fourth group was represented by the genus Pantoea, which was previously isolated from plants such as Trifolium pratense L. and red clover (Sturz et al, 1997), C. arabica L. (Vega et al, 2005), potato (Sessitsch et al, 2004), Calystegia soldanella (Park et al, 2005), maize (Rijavec et al, 2007), citrus (Sturz et al, 1997;Araújo et al, 2001), and peas (Elvira-Recuenco and van Vuurde, 2000). Sessitsch et al (2004) showed that the genus Pantoea produced siderophore and indole-3-acetic acid.…”

“…Upon phylogenetic analysis, the clade Enterobacteriales, with 100% of BP, was divided in two other clades, where isolates 7 (98% of Blast identity to Pantoea conspicua) and 3 (98% Blast identity to Pantoea agglomerans) were grouped. Bacon and Hinton (1996) described that the fifth genus Pseudomonas with 6.25% of total isolates, is a widely distributed plant-associated bacterium, found in the majority of plant species and tissues, often with positive effects on plant growth, such as in Norway spruce (Cankar et al, 2005), C. arabica L. (Vega et al, 2005), potato (Reiter et al, 2002;Sessitsch et al, 2004), C. soldanella and Elymus mollis (Park et al, 2005), Glycine max (Kuklinsky-Sobral et al, 2005).…”

Novel Endophytic Trichoderma spp. Isolated from Healthy Coffea arabica Roots are Capable of Controlling Coffee Tracheomycosis

“…Despite the potential benefit for C. arabica, the community of its endophytic microorganisms has not received much attention. In fact, the only studies so far have been focusing on endophytic bacteria [41], fungal foliar endosymbionts [8] and the presence of ochratoxin producing Penicillium spp. [42] in C. arabica.…”