Endophytic Bacillus altitudinis Strain Uses Different Novelty Molecular Pathways to Enhance Plant Growth

Zhang, Dening; Xu, Hongmin; Gao, Jingyao; Portieles, Roxana; Du, Lihua; Gao, Xiangyou; Nordelo, Carlos Borroto; Borrás‐Hidalgo, Orlando

doi:10.3389/fmicb.2021.692313

Cited by 26 publications

(24 citation statements)

References 77 publications

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“…Many endophytic bacteria have a broad host range. For example, Bacillus altitudinis, isolated from the wild plant species, Glyceria chinensis , can promote the growth of Arabidopsis thaliana , N. tabacum , corn, and soybean 59 . Bacillus megaterium RmBm31, an endophytic bacterium isolated from Retama monosperma , increases the biomass of A. thaliana 60 .…”

Section: Resultsmentioning

confidence: 99%

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

Jiang

Peng

Seo

et al. 2022

Sci Rep

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A novel Gram-stain-positive, aerobic bacterial strain, designated AK-R2A1-2 T, was isolated from the surface-sterilized needle leaves of an Abies koreana tree. Strain AK-R2A1-2 T had 97.3% and 96.7% 16S rRNA gene sequence similarities with Subtercola boreus K300T and Subtercola lobariae 9583bT, respectively, but formed a distinct phyletic lineage from these two strains. Growth of strain AK-R2A1-2 T was observed at 4–25 °C at pH 5.0–8.0. Strain AK-R2A1-2 T contained menaquinone 9 (MK-9) and menaquinone 10 (MK-10) as the predominant respiratory quinones. The major cellular fatty acids were anteiso-C15:0 and summed feature 8 (C18:1ω7c or/and C18:1ω6c), and the polar lipids included diphosphatidylglycerol (DPG) and three unknown aminolipids, AKL2, AKL3, and AKL4. The complete genome of strain AK-R2A1-2 T was sequenced to understand the genetic basis of its survival at low temperatures. Multiple copies of cold-associated genes involved in cold-active chaperon, stress response, and DNA repair supported survival of the strain at low temperatures. Strain AK-R2A1-2 T was also able to significantly improve rice seedling growth under low temperatures. Thus, this strain represents a novel species of the genus Subtercola, and the proposed name is Subtercola endophyticus sp. nov. The type strain is AK-R2A1-2 T (= KCTC 49721 T = GDMCC 1.2921 T).

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Section: Resultsmentioning

confidence: 99%

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

Jiang

Peng

Seo

et al. 2022

Sci Rep

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“…We observed high induction of maturase K gene expression during the interaction. Recent studies revealed that the maturase K gene is induced during the Arabidopsis-Bacillus altitudinis interaction [54].…”

Section: Discussionmentioning

confidence: 99%

Endophytic bacterium Bacillus aryabhattai induces novel transcriptomic changes to stimulate plant growth.

Xu¹,

Gao²,

Portieles³

et al. 2022

Preprint

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In nature, plants interact with a wide range of microorganisms, and most of these microorganisms have the ability to promote plant growth through the induction of important molecular pathways. The current study evaluated whether the endophytic bacterium Bacillus aryabhattai encourages plant growth and the transcriptional changes that might be implicated in this effect. The endophytic bacterium promotes the growth of Arabidopsis and tobacco plants. The transcriptional changes in Arabidopsis plants treated with the bacterium were also identified, and the results showed that various genes, such as cinnamyl alcohol dehydrogenase, apyrase, thioredoxin H8, benzaldehyde dehydrogenase, indoleacetaldoxime dehydratase, berberine bridge enzyme-like and gibberellin-regulated protein, were highly expressed. Also, endophytic bacterial genes, such as arginine decarboxylase, D-hydantoinase, ATP synthase gamma chain and 2-hydroxyhexa-2,4-dienoate hydratase, were activated during the interaction. These findings demonstrate that the expression of novel plant growth-related genes is induced by interaction with the endophytic bacterium B. aryabhattai and that these changes may promote plant growth in sustainable agriculture.

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“…However, the specific contributions of plant-growth promoting Bacillus to indirect inducible defense signaling are unknown. Bacillus altitudinis has be recorded displaying plant beneficial activity through growth promotion (seed yield and phenology) in maize ( Elfira et al, 2020 ; Zhang et al, 2021 ). Zhang et al (2021) reported maize inoculated with B. altitudinis showed increased fresh weight (g) and size ( Zhang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Bacillus altitudinis has be recorded displaying plant beneficial activity through growth promotion (seed yield and phenology) in maize ( Elfira et al, 2020 ; Zhang et al, 2021 ). Zhang et al (2021) reported maize inoculated with B. altitudinis showed increased fresh weight (g) and size ( Zhang et al, 2021 ). In Miscanthus x giganteus and wheat ( Triticum aestivum ), B. altitudinis maintained biomass yield during growth in metal contaminated soils ( Pranaw et al, 2020 ; Yue et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…In Miscanthus x giganteus and wheat ( Triticum aestivum ), B. altitudinis maintained biomass yield during growth in metal contaminated soils ( Pranaw et al, 2020 ; Yue et al, 2021 ). B. altitudinis application upregulated stress tolerance genes in numerous plant species, like phenylpropanoid and lipoxygenase synthesis genes ( Yue et al, 2021 ; Zhang et al, 2021 ), and produces indole acetic acid ( Sun et al, 2017 ), indicating it has the capacity to modulate biochemical pathways important for inducible defense metabolite production. However, it is currently unknown if B. altitudinis has the capacity to modify herbivore-induced plant volatiles (HIPV), and if its preferred functions are observable within the rhizosphere microbiome.…”

Section: Introductionmentioning

confidence: 99%

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Herbivory Protection via Volatile Organic Compounds Is Influenced by Maize Genotype, Not Bacillus altitudinis-Enriched Bacterial Communities

2022

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Belowground, plants interact with beneficial soil microbes such as plant growth-promoting rhizobacteria (PGPR). PGPR are rhizosphere bacteria that colonize roots and elicit beneficial effects in plants such as improved plant growth, pathogen resistance, abiotic stress tolerance, and herbivore protection. Treatment of plants with PGPR has been shown to trigger the emission of volatile organic compounds (VOCs). Volatile emissions can also be triggered by herbivory, termed herbivore-induced plant volatiles (HIPV), with important ramifications for chemical-mediated plant and insect interactions. Much of our current understanding of PGPR and herbivore-induced volatiles is based on studies using one plant genotype, yet domestication and modern breeding has led to the development of diverse germplasm with altered phenotypes and chemistry. In this study, we investigated if volatile emissions triggered by PGPR colonization and herbivory varies by maize genotype and microbial community assemblages. Six maize genotypes representing three decades of crop breeding and two heterotic groups were used, with four microbiome treatments: live or sterilized soil, with or without a Bacillus inoculant. Soil sterilization was used to delay microbiome establishment, resulting in low-diversity treatments. At planting, maize seeds were inoculated with PGPR Bacillus altitudinis AP-283 and grown under greenhouse conditions. Four weeks post planting, plants were subjected to feeding by third instar Helicoverpa zea (Lepidoptera: Noctuidae) larvae. Volatiles were collected using solid phase microextraction and analyzed with gas chromatography-mass spectrometry. Illumina NovaSeq 16S rRNA amplicon sequencing was carried out to characterize the rhizosphere microbiome. Maize genotype significantly influenced total volatile emissions, and relative abundance of volatile classes. We did not document a strong influence of microbe treatment on plant VOC emissions. However, inoculating plants with PGPR improved plant growth under sterile conditions. Taken together, our results suggest that genotypic variation is the dominant driver in HIPV composition and individual HIPV abundances, and any bacterial-mediated benefit is genotype and HIPV-specific. Therefore, understanding the interplay of these factors is necessary to fully harness microbially-mediated benefits and improve agricultural sustainability.

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Endophytic Bacillus altitudinis Strain Uses Different Novelty Molecular Pathways to Enhance Plant Growth

Cited by 26 publications

References 77 publications

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

Endophytic bacterium Bacillus aryabhattai induces novel transcriptomic changes to stimulate plant growth.

Herbivory Protection via Volatile Organic Compounds Is Influenced by Maize Genotype, Not Bacillus altitudinis-Enriched Bacterial Communities

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