Endophyte-produced antimicrobials: a review of potential lead compounds with a focus on quorum-sensing disruptors

Joo, Hwang-Soo; Deyrup, Stephen T.; Shim, Sang Hee

doi:10.1007/s11101-020-09711-7

Cited by 22 publications

(14 citation statements)

References 121 publications

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“…The antibacterial substances produced by specific strains also differ. Antibacterial substances that inhibit pathogens tend not to act alone, but instead two or more antibacterial substances act synergistically to produce antibacterial effects ( Hyakumachi et al, 2014 ; Carrión et al, 2019 ; Joo et al, 2020 ; Newman and Cragg, 2020 ; Armin et al, 2021 ). 2,4-Diacetylphloroglucinol (DAPG) and phenazines (PHZ) produced by Pseudomonas species have been used as antibiotics to control disease suppressive soils and Fusarium wilt ( Weller et al, 2002 ; Haas and Defago, 2005 ; Mazurier et al, 2009 ; Raaijmakers and Mazzola, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

et al. 2021

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Apple replant disease (ARD) is a common problem in major apple planting areas, and biological factors play a leading role in its etiology. Here, we isolated the bacterial strain QSB-6 from the rhizosphere soil of healthy apple trees in a replanted orchard using the serial dilution method. Strain QSB-6 was provisionally identified as Bacillus amyloliquefaciens based on its morphology, physiological and biochemical characteristics, carbon source utilization, and chemical sensitivity. Maximum likelihood analysis based on four gene sequences [16S ribosomal RNA gene (16S rDNA), DNA gyrase subunit A (gyrA), DNA gyrase subunit B (gyrB), and RNA polymerase subunit B (rpoB)] from QSB-6 and other strains indicated that it had 100% homology with B. amyloliquefaciens, thereby confirming its identification. Flat standoff tests showed that strain QSB-6 had a strong inhibitory effect on Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Fusarium oxysporum, Alternaria alternata, Aspergillus flavus, Phoma sp., Valsa mali, Rhizoctonia solani, Penicillium brasilianum, and Albifimbria verrucaria, and it had broad-spectrum antibacterial characteristics. Extracellular metabolites from strain QSB-6 showed a strong inhibitory effect on Fusarium hyphal growth and spore germination, causing irregular swelling, atrophy, rupture, and cytoplasmic leakage of fungal hyphae. Analysis of its metabolites showed that 1,2-benzenedicarboxylic acid and benzeneacetic acid, 3- hydroxy-, methyl ester had good inhibitory effects on Fusarium, and increased the length of primary roots and the number of lateral roots of Arabidopsis thaliana plantlet. Pot experiments demonstrated that a QSB-6 bacterial fertilizer treatment (T2) significantly improved the growth of Malus hupehensis Rehd. seedlings. It increased root length, surface area, tips, and forks, respiration rate, protective enzyme activities, and the number of soil bacteria while reducing the number of soil fungi. Fermentation broth from strain QSB-6 effectively prevented root damage from Fusarium. terminal restriction fragment length polymorphism (T-RFLP) and quantitative PCR (qPCR) assays showed that the T2 treatment significantly reduced the abundance of Fusarium in the soil and altered the soil fungal community structure. In summary, B. amyloliquefaciens QSB-6 has a good inhibitory effect on Fusarium in the soil and can significantly promote plant root growth. It has great potential as a biological control agent against ARD.

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Section: Discussionmentioning

confidence: 99%

Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

et al. 2021

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“…In the last few decades, endophytic fungi, which reside in plant tissues without showing any disease symptoms, have gained extensive attention due to their ability to produce kinds of secondary metabolites ( Manganyi and Ateba, 2020 ). The natural products derived from fungal endophytes possessed a variety of biological properties, such as antimicrobial ( Joo et al, 2021 ), antiparasitic ( Moreno et al, 2011 ), antioxidant ( Mishra et al, 2020 ), immunosuppressant ( Wang et al, 2019 ), and anticancer activities ( Li et al, 2018 ; Uzma et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1

Zhang

Liu

et al. 2022

Front. Microbiol.

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Endophytic fungi of medicinal plants are important sources of active natural products. In this study, 26 fungi were isolated from Artemisia argyi, which were belonging to eight genera, namely, Alternaria, Fusarium, Chaetomium, Phoma, Diaporthe, Trichoderma, Gibberella, and Colletotrichum. The antimicrobial activities of all fungal extracts were tested by using the cup-plate method against Staphylococcus aureus, Salmonella enteritidis, and Fusarium graminearum. The results demonstrated that 25 extracts (96%) exhibited inhibitory activity against at least one of the tested pathogenic microorganisms. The strain Diaporthe sp. AC1, which showed good antimicrobial activity and high yield of crude extract from fermentation, was selected for the study of secondary metabolites. The crude extract of strain AC1 was purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC, and finally, a new compound phomopsolide G (1), together with three known phomopsolides (2–4) and four other known compounds (5–8), was obtained. The structures of the compounds were elucidated by NMR and/or HR-MS spectroscopy. Microdilution method and MTT colorimetry were used to determine the bioactivity of the compounds. The study demonstrated that the new compound 1 had moderate antifungal activity against F. graminearum, Fusarium moniliforme, and Botrytis cinerea and weak antibacterial activity against Staphylococcus aureus. Compound 1 also showed weak cytotoxicity against HepG2, A549, and MDA-MB-231, with IC50 values of 89.91, 107.65, and 53.97 μM. Additionally, other compounds also exhibited antimicrobial and/or cytotoxic activities. The findings provided the basis for searching drug and agricultural lead compounds from A. argyi-associated fungi resources.

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“…Endophytic microorganisms invade plant tissues without producing any obvious detrimental consequences [ 100 ]. Endophytes can be found in the phyllosphere and rhizosphere and adopt lifestyle that commonly start as epiphytes on the plant surface and gradually change to endophytes by invading the plant tissues.…”

Section: Microbial Interactions and Chemical Signalling In Plantmentioning

confidence: 99%

Microbe Related Chemical Signalling and Its Application in Agriculture

Hamid

Nadarajah

2022

IJMS

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The agriculture sector has been put under tremendous strain by the world’s growing population. The use of fertilizers and pesticides in conventional farming has had a negative impact on the environment and human health. Sustainable agriculture attempts to maintain productivity, while protecting the environment and feeding the global population. The importance of soil-dwelling microbial populations in overcoming these issues cannot be overstated. Various processes such as rhizospheric competence, antibiosis, release of enzymes, and induction of systemic resistance in host plants are all used by microbes to influence plant-microbe interactions. These processes are largely founded on chemical signalling. Producing, releasing, detecting, and responding to chemicals are all part of chemical signalling. Different microbes released distinct sorts of chemical signal molecules which interacts with the environment and hosts. Microbial chemicals affect symbiosis, virulence, competence, conjugation, antibiotic production, motility, sporulation, and biofilm growth, to name a few. We present an in-depth overview of chemical signalling between bacteria-bacteria, bacteria-fungi, and plant-microbe and the diverse roles played by these compounds in plant microbe interactions. These compounds’ current and potential uses and significance in agriculture have been highlighted.

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Endophyte-produced antimicrobials: a review of potential lead compounds with a focus on quorum-sensing disruptors

Cited by 22 publications

References 121 publications

Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1

Microbe Related Chemical Signalling and Its Application in Agriculture

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