Endophyte Enzymes and Their Applications in Industries

Toghueo, Rufin Marie Kouipou; Boyom, Fabrice Fekam

doi:10.1002/9781119718017.ch6

Cited by 4 publications

(2 citation statements)

References 109 publications

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“…Endophytic microbes can stand as a potential source of extracellular enzymes for industrial purposes due to catalytic activity, thermostability, low cost, organic substrates availability, etc. (Toghueo and Boyom, 2021). Screening of extracellular enzymes, such as cellulases, proteases, xylanases, chitinases, and xylanases from plant microbes has been documented (Alkahtani et al, 2020;Blibech et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

In vitro Screening of Sunflower Associated Endophytic Bacteria With Plant Growth-Promoting Traits

Adeleke

Babalola²

2022

Front. Sustain. Food Syst.

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Harnessing endophytic microbes as bioinoculants promises to solve agricultural problems and improve crop yield. Out of fifty endophytic bacteria of sunflowers, 20 were selected based on plant growth-promoting. These plant growth-promoting bacteria were identified as Bacillus, Pseudomonas, and Stenotrophomonas. The qualitative screening showed bacterial ability to produce hydrogen cyanide, ammonia, siderophore, indole-3-acetic acid (IAA), exopolysaccharide, and solubilize phosphate. The high quantity of siderophore produced by B. cereus T4S was 87.73%. No significant difference was observed in the Bacillus sp. CAL14 (33.83%), S. indicatrix BOVIS40 (32.81%), S. maltophilia JVB5 (32.20%), S. maltophilia PK60 (33.48%), B. subtilis VS52 (33.43%), and P. saponiphilia J4R (33.24%), exhibiting high phosphate-solubilizing potential. S. indicatrix BOVIS40, B. thuringiensis SFL02, B. cereus SFR35, B. cereus BLBS20, and B. albus TSN29 showed high potential for the screened enzymes. Varied IAA production was recorded under optimized conditions. The medium amended with yeast extract yielded high IAA production of 46.43 μg/ml by S. indicatrix BOVIS40. Optimum IAA production of 23.36 and 20.72 μg/ml at 5% sucrose and 3% glucose by S. maltophilia JVB5 and B. cereus T4S were recorded. At pH 7, maximum IAA production of 25.36 μg/ml was obtained by S. indicatrix BOVIS40. All the isolates exhibited high IAA production at temperatures 25, 30, and 37°C. The in vitro seed inoculation enhanced sunflower seedlings compared to the control. Therefore, exploration of copious endophytic bacteria as bioinoculants can best be promising to boost sunflower cultivation.

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Section: Discussionmentioning

confidence: 99%

In vitro Screening of Sunflower Associated Endophytic Bacteria With Plant Growth-Promoting Traits

Adeleke

Babalola²

2022

Front. Sustain. Food Syst.

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“…All plant species have endophytic microorganisms, such as bacteria, actinomycetes, and fungi, that are constantly present within their tissues, without causing any injury or disease [8]. These microorganisms are used in many different industries, such as agriculture, industry, and medicine, since they are thought of as a reservoir that hold many biologically active compounds [9][10][11][12]. Endophytes develop symbiotic relationships with several plant species and have the ability to control a wide range of host functions, including immune system stimulation, growth and development, and resistance to abiotic and biotic challenges [13,14].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Endophytes from Moringa oleifera Leaves as a Promising Source for Bioactive Compounds

et al. 2023

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Bacterial endophytes reside within the tissues of living plant species without causing any harm or disease to their hosts. Bacterial endophytes have produced a variety of bioactive compounds that can be used for different biomedical applications. In the current study, two bacterial endophytes were isolated from healthy Moringa oleifera leaves, and identified genetically as Stenotrophomonas maltophilia and Alcaligenes faecalis. Phytochemical results illustrated that A. faecalis produced phenolics at 547.2 mg/g, tannins at 156.7 µg/g, flavonoids at 32.8 µg/g, and alkaloids at 111.2 µg/g compared to S. maltophilia, which produced phenolics at 299.5 mg/g, tannins at 78.2 µg/g, flavonoids at 12.4 µg/g, and alkaloids at 29.4 µg/g. GC-MS analysis indicated that A. faecalis extract has 24 bioactive compounds, including 9 major compounds, namely octadecanoic acid, hexadecanoic acid, linoleic acid ethyl ester, octadecenoic acid, methyl ester, methyl stearate, nonacosane, indolizine, palmitoleic acid, and heptacosane. On the other hand, S. maltophilia extract has 11 bioactive compounds, including 8 major compounds, namely oleic acid, octadecanoic acid, hexadecanoic acid, cis-2-phenyl-1, 3-dioxolane-4-methyl, ergotamine, diisooctyl phthalate, diethyl phthalate, and pentadecanoic acid. To check the safety of these extracts, the cytotoxicity of Ethyl acetate (EA) extracts of S. maltophilia and A. faecalis were evaluated against the Vero normal cell line, and the results confirmed that these extracts are safe to use. Moreover, results revealed that EA extracts of S. maltophilia and A. faecalis exhibited anticancer activity against the cancerous MCF7 cell line, where IC50 was 202.4 and 119.7 µg/mL, respectively. Furthermore, EA extracts of S. maltophilia had antibacterial and antifungal activity against Gram-positive and Gram-negative bacteria, and unicellular fungi. Likewise, the EA extract of A. faecalis exhibited antibacterial and antifungal activity against Gram-positive bacteria, as well as unicellular fungi, but did not show any activity against Gram-negative bacteria. Also, EA extracts of S. maltophilia and A. faecalis exhibited moderate antioxidant activity where IC50 were 146.2 and 147.6 µg/mL, respectively. In conclusion, the two isolated endophytic bacteria S. maltophilia and A. faecalis have promising bioactive compounds that have antibacterial, antioxidant, and anticancer activities.

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Meta-omics of endophytic microbes in agricultural biotechnology

Adeleke

Babalola

2022

Biocatalysis and Agricultural Biotechnology

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Endophyte Enzymes and Their Applications in Industries

Cited by 4 publications

References 109 publications

In vitro Screening of Sunflower Associated Endophytic Bacteria With Plant Growth-Promoting Traits

In vitro Screening of Sunflower Associated Endophytic Bacteria With Plant Growth-Promoting Traits

Bacterial Endophytes from Moringa oleifera Leaves as a Promising Source for Bioactive Compounds

Meta-omics of endophytic microbes in agricultural biotechnology

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