Endoparasitism in cereal aphids: molecular analysis of a whole parasitoid community

Traugott, Michael; Bell, James R.; Broad, Gavin R.; Powell, W.; Veen, F. J. Frank van; Vollhardt, Ines M. G.; Symondson, William Oliver Christian

doi:10.1111/j.1365-294x.2008.03878.x

Cited by 90 publications

(115 citation statements)

References 61 publications

(79 reference statements)

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…However, the lake of variability in 28S gene suggests that it should be accompanied with other gene markers or morphological traits. Molecular tools based on DNA sequences of certain locus have been successfully used to detect immature stages of parasitoids inside aphids (Traugott et al 2008). The recommendation for future work on the parasitoids is to employ specific primers for correct detection of Aphidiinae inside A. punicae.…”

Section: Resultsmentioning

confidence: 99%

Molecular and morphological identification of hymenoptran parasitoids from the pomegranate aphid, Aphis punicae in Razavi Khorasan province, Iran

Farrokhzadeh¹,

Moravvej²,

Awal³

et al. 2014

Turkish Journal of Entomology

View full text Add to dashboard Cite

Parasitoids play the positive role in aphids control management by keeping the population of aphids below the economic threshold. The pomegranate aphid, Aphis punicae is attacked by several parasitoids which mostly belong to Aphidiinae subfamily. The identification of parasitoids and hyperparasitoids is problematic due to their small size and ambiguous morphological characteristics. The analysis of molecular data of DNA sequences has been suggested as a complementary approach to the classical methods to identify biocontrol agents. In the present study, active parasitoids and hyperparasitoids of the pomegranate aphid collected from Razavi Khorasan province were identified using morphological characters and analysis of two gene sequences including COI and 28S. Phylogenetic analysis was performed using Maximum parsimony and Bayesian methods. The identified parasitoids were Binodoxys angelicae, Lysiphlebus fabarum, Ephedrus persicae, and the hyperparasitoids were Syrphophagus aphidivorus, Alloxysta sp. and Pachyneuron sp. We demonstrated that the mitochondrial COI gene was superior for the identification of these insects compared to the nuclear 28S (D2-region) and that it was compatible with morphological studies. This is the first study on molecular identification of the parasitoids and hyperparasitoids on pomegranate aphids in Iran.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular and morphological identification of hymenoptran parasitoids from the pomegranate aphid, Aphis punicae in Razavi Khorasan province, Iran

Farrokhzadeh¹,

Moravvej²,

Awal³

et al. 2014

Turkish Journal of Entomology

View full text Add to dashboard Cite

show abstract

“…Unlike multiplex PCRs, which are often used in aphid food web studies (e.g. Traugott et al 2008, Gómez-Marco et al 2015 and the success of which relies on a thorough knowledge of the interacting species, high throughput DNA sequencing tools also allow the discovery of new species and new species associations. In a context of climate change which has the potential to highly disrupt the structure and the functioning of local food webs and the outbreak of pests, it is essential that the tools used to understand these food webs allow us, as much as possible, to get a complete picture of the scene.…”

Section: Resultsmentioning

confidence: 99%

“…It has been previously shown that parasitoid and hyperparasitoid DNA can be easily retrieved from aphid mummies in which the parasitoid or hyperparasitoid has developed (Traugott et al 2008, Derocles et al 2012, Gariepy et al 2013). In empty mummies, a large part of parasitoids (and hyperparasitoids) residual DNA may come from their faecal pellet, meconium (Haviland 1922, El-Heneidy and Adly 2015) and/or exuviae (Lefort et al 2012).…”

Section: Discussion Amplification Success and Detection Of Trophic Lementioning

confidence: 99%

See 1 more Smart Citation

Disentangling higher trophic level interactions in the cabbage aphid food web using high-throughput DNA sequencing

Lefort¹,

Wratten²,

Cusumano³

et al. 2017

Metabarcoding and Metagenomics

View full text Add to dashboard Cite

The lack of understanding of complex food-web interactions has been a major gap in the history of biological control. In particular, a better understanding of the functioning of pest food-webs and how they vary between native and invaded geographical ranges is of prime interest for biological control research and associated integrated pest management. Technical limitations associated with the deciphering of complex food-webs can now be largely overcome by the use of high throughput DNA sequencing techniques such as Illumina MiSeq. We tested the efficiency of this next generation sequencing technology in a metabarcoding approach, to study aphid food-webs using the cabbage aphid as model. We compared the variations in structure and composition of aphid food-webs in the species' native range (United Kingdom, UK) and in an invaded range (New Zealand, NZ). We showed that Illumina MiSeq is a well suited technology to study complex aphid food-webs from aphid mummies. We found an unexpectedly high top down pressure in the NZ cabbage aphid food-web, which coupled to a large ratio of consumer species / prey ‡, § § species and a lack of potential inter-specific competition between primary parasitoids, could cause the NZ food-web to be more vulnerable than the UK one. This study also reports for the first time the occurrence of a new hyperparasitoid species in NZ, as well as new associations between hyperparasitoids parasitoids and the cabbage aphid in this country. We conclude that the complexity of aphid food-webs in agricultural systems could often be underestimated, particularly at higher trophic levels; and that the use of high throughput DNA sequencing tools, could largely help to overcome this impediment.

show abstract

“…Samples of S. avenae stored in 99% ethanol were analyzed first by extracting DNA using the Salting Out protocol (Sunnucks and Hales, 1996) and then using a multiplex PCR test as described by Traugott et al (2008). Species-specific primers developed by Traugott et al (2008) for accurately identifying the immature stages (eggs, larvae and pupae) of 10 parasitoid species associated with S. avenae were used.…”

Section: Assessment Of Parasitoid Species Using Molecular Methodsmentioning

confidence: 99%

Abundancia y prevalencia de Aphidius avenae (Hymenoptera: Braconidae: Aphidiinae) en Chile

Peñalver‐Cruz

Ortiz-Martínez

Villegas

et al. 2017

Ciencia e investigación agraria

View full text Add to dashboard Cite

A. Peñalver-Cruz, S. Ortiz-Martínez, C. Villegas, Ž. Tomanović, F. Zepeda-Paulo, V. Žikić, and B. Lavandero. 2017. Abundance and prevalence of Aphidius avenae (Hymenoptera: Braconidae: Aphidiinae) in Chile. Cien. Inv. Agr. 44(2): 207-214. During the samplings described here, Aphidius avenae (=picipes) (Haliday, 1834) was collected. This species has been reported in Chile but has never been studied in terms of its relative abundance and prevalence in central and southern Chile by using classical or molecular methods. The composition of the parasitoid assemblages on several cereal aphid hosts was examined for three seasons in agricultural landscapes. In this note, we describe the prevalence of this parasitoid species in cereal aphids and other aphid hosts and discuss the abundance of this parasitoid compared to other aphid parasitoids and the probable origins of this important aphid parasitoid species in Chile. The results indicate that A. avenae has a low prevalence compared to other main parasitoids such as Aphidius ervi (Haliday) and that it has been found parasitizing the grain aphid (Sitobion avenae (Fabricius)) and the bird cherry-oat aphid (Rhopalosiphum padi (Linnaeus)) in wheat and the foxglove aphid (Aulacorthum solani (Kaltenbach)) in tomato. Although the introduction of A. avenae in Chile as a biological agent in 1992 was not successful, we here confirm the presence of this aphid parasitoid wasp in Chile and discuss the factors that could explain the low prevalence of this parasitoid species in the country.

show abstract

Endoparasitism in cereal aphids: molecular analysis of a whole parasitoid community

Cited by 90 publications

References 61 publications

Molecular and morphological identification of hymenoptran parasitoids from the pomegranate aphid, Aphis punicae in Razavi Khorasan province, Iran

Molecular and morphological identification of hymenoptran parasitoids from the pomegranate aphid, Aphis punicae in Razavi Khorasan province, Iran

Disentangling higher trophic level interactions in the cabbage aphid food web using high-throughput DNA sequencing

Abundancia y prevalencia de Aphidius avenae (Hymenoptera: Braconidae: Aphidiinae) en Chile

Contact Info

Product

Resources

About