Endoneurial extracellular matrix influences regeneration and maturation of motor nerve axons—A model of acellular nerve graft

Kubek, Tomáš; Ghalib, Nabil Ahmed; Dubový, Petr

doi:10.1016/j.neulet.2011.03.085

Cited by 5 publications

(1 citation statement)

References 31 publications

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“…12 Earlier studies revealed that the implantation of BM-MSCs and SCs within ANGs can enhance the regeneration of sciatic nerve axons compared with ANGs. [46][47][48][49][50][51][52] Tooth-derived stem cells exhibited BMSC-like multipotency and cell surface marker expression; however, they expressed a distinct set of multiple early neural linage markers. 53 Dental pulp is derived from the neural crest and has been shown to produce a large number of neurotrophic factors during development, such as neurotrophins and GDNF.…”

Section: Discussionmentioning

confidence: 99%

DPSCs seeded in acellular nerve grafts processed by Myroilysin improve nerve regeneration

Qiao

et al. 2018

J Biomater Appl

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Since synthetic nerve conduits do not exhibit ideal regeneration characteristics, they are generally inadequate substitutes for autologous nerve grafts in the repair of long peripheral nerve defects. To resolve this problem, in this study, a nerve regeneration acellular nerve graft (ANG) with homologous dental pulp stem cells (DPSCs) was constructed. Xenogeneic ANG was processed by Myroilysin to completely remove cells and myelin sheath, while preserving extracellular matrix (ECM) microstructure of the natural nerve. The study revealed that ANG could support cell attachment and proliferation and did not stimulate a vigorous host rejection response. After inoculation of rabbit DPSCs (r-DPSCs) onto ANG, cells were observed to align along the longitudinal axis of the acellular nerve matrix (ANM) and persistently express NGF and BDNF. Undifferentiated r-DPSCs also presented glial cell characteristics and promoted nerve regeneration after transplantation in vivo. We repaired 1 cm purebred New Zealand White Rabbits sciatic nerve defects using this nerve graft construction, and nerve gap regeneration was indicated by electrophysiological and histological analysis. Therefore, we conclude that the combination of an ANG processed by Myroilysin with DPSCs providing a microenvironment that increases nerve regeneration for repairing peripheral nerve defects.

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Section: Discussionmentioning

confidence: 99%

DPSCs seeded in acellular nerve grafts processed by Myroilysin improve nerve regeneration

Qiao

et al. 2018

J Biomater Appl

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Different Forms of Decellularized Tissues and Their Characteristics, Applications in Tissue Repair as Well as Performance Optimization

Lei

Song

et al. 2021

Decellularized Materials

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Immunoregulation Effects of Bone Marrow-Derived Mesenchymal Stem Cells in Xenogeneic Acellular Nerve Grafts Transplant

Fan

et al. 2014

Cell Mol Neurobiol

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This study evaluated whether bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with xenogeneic acellular nerve grafts (xANGs) would reduce the inflammation reaction of xANGs transplantation. BM-MSCs were extracted, separated, purified, and cultured from the bone marrow of rats. Then BM-MSCs were seeded into 5 mm xANGs as experimental group, while xANGs group was chosen as control. Subcutaneous implantation and nerve grafts transplantation were done in this study. Walking-track tests, electrophysiological tests, H&E staining, and immunostaining of CD4, CD8, and CD68 of subcutaneous implantations, cytokine concentrations of IL-2, IL-10, IFN-γ and TNF-α in lymphocytes supernatants and serum of the two groups were evaluated. Walking-track tests and electrophysiological tests suggested the group of BM-MSCs with xANGs obtained better results than xANGs group (P < 0.05). H&E staining and immunostaining of CD4, CD8, and CD68 of subcutaneous implantations showed there were less inflammatory cells in the group of BM-MSCs when compared with the xANGs group. The cytokine concentrations of IL-2, IFN-γ, and TNF-α in BM-MSCs group were lower than xANGs group in lymphocytes supernatants and serum (P < 0.05). However, IL-10 concentrations in BM-MSCs group were higher than xANGs group (P < 0.05). xANG with BM-MSCs showed better nerve repair function when compared with xANG group. Furthermore, xANG with BM-MSCs showed less inflammatory reaction which might indicate the reason of its better nerve regeneration.

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Endoneurial extracellular matrix influences regeneration and maturation of motor nerve axons—A model of acellular nerve graft

Cited by 5 publications

References 31 publications

DPSCs seeded in acellular nerve grafts processed by Myroilysin improve nerve regeneration

DPSCs seeded in acellular nerve grafts processed by Myroilysin improve nerve regeneration

Different Forms of Decellularized Tissues and Their Characteristics, Applications in Tissue Repair as Well as Performance Optimization

Immunoregulation Effects of Bone Marrow-Derived Mesenchymal Stem Cells in Xenogeneic Acellular Nerve Grafts Transplant

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