Endomicroscopic analysis of time- and pressure-dependent area of subpleural alveoli in mechanically ventilated rats

Runck, Hanna; Schwenninger, David; Haberstroh, Jörg; Guttmann, J.

doi:10.1016/j.resp.2014.08.010

Cited by 2 publications

(4 citation statements)

References 29 publications

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“…21 Several studies exist which use relative values of alveolar area change to quantify lung tissue dynamics via IVM. 4,13 Here, it is still important to notice that the position of the brightest reflex changes with the axial position of the neighboring alveolus, as we have discussed above. During respiration, the measured size of the alveolus might change just due to relative axial movement within the tissue unless the inner onset of the bright reflex is used.…”

Section: Journal Of Biomedical Optics 066009-6mentioning

confidence: 83%

“…The bright reflexes are currently used as a border for the discrimination between alveolar lumen and tissue. [2][3][4]7 At the transition to liquid filling, the bright reflexes show decreased intensity at the side facing the liquidfilled area compared to the side facing the air-filled region [asterisk in Fig. 4(a)].…”

Section: Experimental Investigations On Liquid-filled Lung Tissuementioning

confidence: 99%

“…To avoid a direct reflex from the pleura of the lung, the illumination is performed under a certain angle of incidence. The current opinion in IVM studies is that the reflexes visible in the camera image arise from the alveolar walls [2][3][4] or partial reflection at the boundary between tissue and alveolar lumen, which is filled with air. 7 Since the distance between the bright reflexes of neighboring alveoli is greater as would be expected for an alveolar septum, the latter hypothesis of partial reflection seems to be more reasonable.…”

Section: Theoretical Considerationsmentioning

confidence: 99%

“…On an alveolar level, we find animal studies investigating in vivo the dynamic behavior of lung parenchyma using intravital microscopy (IVM). [1][2][3][4][5][6][7] Quantitative image analysis of such images can provide the mechanical characteristics of alveolar tissue, which can further be used to model lung mechanics in various states of the parenchyma. 8,9 But what exactly do we see on IVM images of alveoli?…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics

et al. 2015

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Intravital microscopy (IVM) is a well-established imaging technique for real-time monitoring of microscale lung tissue dynamics. Although accepted as a gold standard in respiratory research, its characteristic image features are scarcely understood, especially when trying to determine the actual position of alveolar walls. To allow correct interpretation of these images with respect to the true geometry of the lung parenchyma, we analyzed IVM data of alveoli in a mouse model in comparison with simultaneously acquired optical coherence tomography images. Several IVM characteristics, such as double ring structures or disappearing alveoli in regions of liquid filling, could be identified and related to the position of alveoli relative to each other. Utilizing a ray tracing approach based on an idealized geometry of the mouse lung parenchyma, two major reflection processes could be attributed to the IVM image formation: partial reflection and total internal reflection between adjacent alveoli. Considering the origin of the reflexes, a model was developed to determine the true position of alveolar walls within IVM images. These results allow thorough understanding of IVM data and may serve as a basis for the correction of alveolar sizes for more accurate quantitative analysis within future studies of lung tissue dynamics.

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Section: Journal Of Biomedical Optics 066009-6mentioning

confidence: 83%

Section: Experimental Investigations On Liquid-filled Lung Tissuementioning

confidence: 99%

Section: Theoretical Considerationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics

et al. 2015

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Effects of Alveolar Morphology on Alveolar Mechanics: An Experimental Study of Mouse Lung Based on Two- And Three-Dimensional Imaging Methods

Liu

Wang

Liu

et al. 2019

J. Mech. Med. Biol.

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Understanding alveolar mechanics is important for preventing the possible lung injuries during mechanical ventilation. Alveolar clusters with smaller size are found having lower compliance in two-dimensional studies. But the influence of alveolar shape on compliance is unclear. In order to investigate how alveolar morphology affects their behavior, we tracked subpleural alveoli of isolated mouse lungs during quasi-static ventilation using two- and three-dimensional imaging techniques. Results showed that alveolar clusters with smaller size and more spherical shape had lower compliance. There was a better correlation of sphericity rather than circularity with alveolar compliance. The compliance of clusters with great shape change was larger than that with relatively slight shape change. These findings suggest the contribution of lung heterogeneous expansion to lung injuries associated with mechanical ventilation.

show abstract

Endomicroscopic analysis of time- and pressure-dependent area of subpleural alveoli in mechanically ventilated rats

Cited by 2 publications

References 29 publications

Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics

Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics

Effects of Alveolar Morphology on Alveolar Mechanics: An Experimental Study of Mouse Lung Based on Two- And Three-Dimensional Imaging Methods

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