Endometriosis: A Malignant Fingerprint

DeAngelo, Christopher; Tarasiewicz, Megan Burnett; Strother, Athena Marie; Taggart, Heather; Gray, Caron; Shanahan, Meaghan; Glowacki, C; Khandalavala, Jimmy; Talaska, Erin; Kinnan, Andrea; Coté, John Joseph; Edwards, Adrienne Perfilio; Harper-Harrison, Gina; Casey, Murray Joseph; Hirai, Traci-Lynn; Schultz, Sarah; Stines, Lynnea; Vora, Roma; Boudreau, Dominique K.; Burgart, Jennifer; Shama, Meredith; Watson, Trevor; Strasheim, Lisa; Thompson, Rachel H.; Lawlor, Rachel; Joyce, Kayleen; Magnuson, Claire M; Driano, Jane E.; Elger, Breanna; Lentino, Anne; Driscoll, Margaret E.; Tidwell, E; Sharma, Anita; Walker, Sarah R.; Jones, Gretchen; Sharma, Poonam; Stessman, Holly A.F.; Wu, Yanyuan; Vadgama, Jay; Chase, Dana M.; Conrad, Lesley B.; Reddy, Srinivasa T.; Farias‐Eisner, Robin

doi:10.17303/jcrto.2020.8.206

Cited by 3 publications

(2 citation statements)

References 74 publications

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“…Atypical ovarian endometriosis is known to be especially associated with an increased risk of malignancy 13 . Currently, molecular mechanisms and biomarkers are being carefully investigated in women with endometriosis to find a diagnostic algorithm for the prediction and possibly prevention or treatment of women with an increased risk of endometriosis-associated malignancies 14 . Histological parameters of cell proliferation may have an additional diagnostic value when assessing the severity of endometriosis but could also serve as predictive markers for the development of malignant epithelial tumors.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Wetzk

Grübling

Forberger

et al. 2022

Geburtshilfe Frauenheilkd

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Objectives Endometriosis is a chronic disease which is diagnosed by surgical intervention combined with a histological work-up. Current international and national recommendations do not require the histological determination of the proliferation rate. The diagnostic and clinical importance of the mitotic rate in endometriotic lesions still remains to be elucidated. Methods In this retrospective study, the mitotic rates and clinical data of 542 patients with histologically diagnosed endometriosis were analyzed. The mean patient age was 33.5 ± 8.0 (17 – 72) years, and the mean reproductive lifespan was 21.2 ± 7.8 (4 – 41) years. Patients were divided into two groups and patientsʼ reproductive history and clinical endometriosis characteristics were compared between groups. The study group consisted of women with confirmed mitotic figures (n = 140, 25.83%) and the control group comprised women without proliferative activity according to their mitotic rates (n = 402, 74.27%). Results Women with endometriotic lesions and histologically confirmed mitotic figures were significantly more likely to have a higher endometriosis stage (p = 0.001), deep infiltrating endometriosis (p < 0.001), ovarian endometrioma (p = 0.012), and infertility (p = 0.049). A mitotic rate > 0 was seen significantly less often in cases with incidental findings of endometriosis (p = 0.031). The presence of symptoms and basic characteristics such as age, age at onset of menarche, reproductive lifespan and parity did not differ between the group with and the group without mitotic figures. Conclusion This study shows that a simple histological assessment of the mitotic rate offers additional diagnostic value for the detection of advanced stages of endometriosis. The possible role as a predictive marker for the recurrence of endometriosis or the development of endometriosis-associated cancer will require future study.

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Section: Introductionmentioning

confidence: 99%

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Wetzk

Grübling

Forberger

et al. 2022

Geburtshilfe Frauenheilkd

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“…Endometriosis presents with dysregulation of multiple genes and proteins [ 45 ], such as enzymes, metabolites, signaling, and immune molecules [ 13 , 46 , 47 ], some of which are still not elucidated. This initial study for biomarker discovery considered patients with all stages of the disease, in order to search for a marker indicative of the condition as a whole.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Soluble CD90: Potential for Diagnostic Significance in Endometriosis Patients

et al. 2022

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Background. Endometriosis is a chronic and debilitating gynecologic disorder, driven by endocrine and immune dysfunctions, which lead to poor endometrial differentiation and attenuated fertility. Escape from immune surveillance and involvement of inflammatory mechanisms appear to be factors in disease progression. Current diagnostic guidelines for endometriosis still lack an efficient biomarker. Here, we report a study on two previously unexplored factors as potential biomarkers for endometriosis. Methods. A case-control study was performed to evaluate the diagnostic potential of serum CD90 and CD83 levels in endometriosis patients (cases validated by surgical and histological examination) compared to healthy controls. Serum was collected from age-matched females and analyzed by ELISA. Results. Comparison of endometriosis patients to the control group showed significantly elevated levels of serum CD90 ( 1160 ± 856 pg/mL vs. 334 ± 228 pg/mL; ∗ ∗ ∗ ∗ p < 0.0001 ). A threshold value of 479.4 pg/mL was defined based on the control results, and the diagnostic efficiency of the test was estimated. The obtained sensitivity (70.4%), specificity (92.9%), positive predictive value (90.5%), and negative predictive value (76.5%) rated the test as one with promising diagnostic potential. In contrast, the analysis of serum CD83 levels showed comparable values in both groups, suggesting no association with patient status. Conclusion. Elevated soluble CD90 in human serum is associated with endometriosis, which suggests its putative clinical significance as a biomarker in screening and/or diagnosis of the disease.

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Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis

et al. 2023

Journal of Immunology Research

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Background. Endometriosis is an inflammatory gynecological disease leading to deep pelvic pain, dyspareunia, and infertility. The pathophysiology of endometriosis is complex and depends on a variety of biological processes and pathways. Therefore, there is an urgent need to identify reliable biomarkers for early detection and accurate diagnosis to predict clinical outcomes and aid in the early intervention of endometriosis. We screened transcription factor- (TF-) immune-related gene (IRG) regulatory networks as potential biomarkers to reveal new molecular subgroups for the early diagnosis of endometriosis. Methods. To explore potential therapeutic targets for endometriosis, the Gene Expression Omnibus (GEO), Immunology Database and Analysis Portal (ImmPort), and TF databases were used to obtain data related to the recognition of differentially expressed genes (DEGs), differentially expressed IRGs (DEIRGs), and differentially expressed TFs (DETFs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on the DETFs and DEIRGs. Then, DETFs and DEIRGs were further validated in the external datasets of GSE51981 and GSE1230103. Then, we used quantitative real-time polymerase chain reaction (qRT-PCR) to verify the hub genes. Simultaneously, the Pearson correlation analysis and protein-protein interaction (PPI) analyses were used to indicate the potential mechanisms of TF-IRGs at the molecular level and obtain hub IRGs. Finally, the receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic value of the hub IRGs. Results. We screened a total of 94 DETFs and 121 DEIRGs in endometriosis. Most downregulated DETFs showed decreased expression in the endometria of moderate/severe endometriosis patients. The top-ranked upregulated DEIRGs were upregulated in the endometra of infertile women. Functional analysis showed that DETFs and DEIRGs may be involved in the biological behaviors and pathways of endometriosis. The TF-IRG PPI network was successfully constructed. Compared with the control group, high C3, VCAM1, ITGB2, and C3AR1 expression had statistical significance in endometriosis among the hub DEIRGs. They also showed higher sensitivity and specificity by ROC analysis for the diagnosis of endometriosis. Finally, compared with controls, C3 and VCAM1 were highly expressed in endometriosis tissue samples. In addition, they also showed high specificity and sensitivity for diagnosing endometriosis. Conclusion. Overall, we discovered the TF-IRG regulatory network and analyzed 4 hub IRGs that were closely related to endometriosis, which contributes to the diagnosis of endometriosis. Additionally, we verified that DETFs or DEIRGs were associated with the clinicopathological features of endometriosis, and external datasets also confirmed the hub IRGs. Finally, C3 and VCAM1 were highly expressed in endometriosis tissue samples compared with controls and may be potential biomarkers of endometriosis, which are helpful for the early diagnosis of endometriosis.

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Endometriosis: A Malignant Fingerprint

Cited by 3 publications

References 74 publications

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Evaluation of Soluble CD90: Potential for Diagnostic Significance in Endometriosis Patients

Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis

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