Endometrial stem cell differentiation into smooth muscle cell: a novel approach for bladder tissue engineering in women

Abstract

Human EnSCs were successfully differentiated into SMCs, using hydrogels as scaffold. EnSCs may be used for autologous bladder wall regeneration without any immunological complications in women. Currently work is in progress using bioabsorbable nanocomposite materials as EnSC scaffolds for developing urinary bladder wall tissue.

Use of regenerative tissue for urinary diversion

“…Additional stem cell populations recently investigated for urinary tissue reconstruction include endometrial stem cells, amniotic fluid stem cells, and hair stem cells [95][96][97][98][99]23]. Similar to prior studies using different cell types, scaffolds seeded with stem cells isolated from the follicular bulge (CD34, p63, and Ki-67 positive) demonstrated UC and SMC differentiation and improved tissue recellularization than a cellular graft alone [98].…”

Hypoxia-Controlled EphA3 Marks a Human Endometrium-Derived Multipotent Mesenchymal Stromal Cell that Supports Vascular Growth

“…Indeed, endometrial-derived MSC-like cells isolated from menstrual blood - also termed “Endometrial Regenerative Cells” - that have multi-lineage differentiation capacity are being evaluated in ongoing Phase-II clinical trials for their cardiovascular regenerative potential [71]. The therapeutic function of eMSCs and related cells is likely multifaceted, including promotion of vascularisation, secretion of trophic factors, and direct differentiation into relevant cell types [70]–[76]. Our present study has identified EphA3 as a new marker of multipotent MSCs in the endometrium, which furthermore is functionally implicated in hypoxia-induced rapid neovascularisation.…”

Technological Innovation in the BJUI

“…Finally, we have an exciting paper from Iran on the use of endometrial derived stem cells for creating bladder replacements and alternatives to meshes in prolapse surgery. The immuno and scanning electron micrographic images in this paper are just stunning .…”