Endometrial prostaglandin synthases, ovarian steroids, and oxytocin receptors in mares with oxytocin-induced luteal maintenance

Rebordão, Maria Rosa; Galvão, António; Pinto‐Bravo, Pedro; Pinheiro, Joana; Gamboa, Sandra; Silva, E.; Mateus, L.; Ferreira‐Dias, Graça

doi:10.1016/j.theriogenology.2016.08.028

Cited by 20 publications

(6 citation statements)

References 47 publications

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“…Estrogen stimulation increases the expression of PDLIM3 in human prostate cancer ( 69 ). However, even if the expression level of PTGIS does not perfectly match that of the ERβ, it may be negatively influenced by ERβ ( 70 , 71 ). We thus propose here the existence of signaling interactions between the validated DEGs including C7, CFH, FZD7, LY96, PDLIM3 , and PTGIS , and pathways including TLR4/NF-κB, Wnt/frizzled, and estrogen receptors ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of novel endometriosis biomarkers via integrative bioinformatics

Bae

Cho

et al. 2022

Front. Endocrinol.

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Endometriosis is a gynecological disease prevalent in women of reproductive age, and it is characterized by the ectopic presence and growth of the eutopic endometrium. The pathophysiology and diagnostic biomarkers of endometriosis have not yet been comprehensively determined. To discover molecular markers and pathways underlying the pathogenesis of endometriosis, we identified differentially expressed genes (DEGs) in three Gene Expression Omnibus microarray datasets (GSE11691, GSE23339, and GSE7305) and performed gene set enrichment analysis (GSEA) and protein–protein interaction (PPI) network analyses. We also validated the identified genes via immunohistochemical analysis of tissues obtained from patients with endometriosis or healthy volunteers. A total of 118 DEGs (79 upregulated and 39 downregulated) were detected in each dataset with a lower (fold change) FC cutoff (log2|FC| > 1), and 17 DEGs (11 upregulated and six downregulated) with a higher FC cutoff (log2|FC| > 2). KEGG and GO functional analyses revealed enrichment of signaling pathways associated with inflammation, complement activation, cell adhesion, and extracellular matrix in endometriotic tissues. Upregulation of seven genes (C7, CFH, FZD7, LY96, PDLIM3, PTGIS, and WISP2) out of 17 was validated via comparison with external gene sets, and protein expression of four genes (LY96, PDLIM3, PTGIS, and WISP2) was further analyzed by immunohistochemistry and western blot analysis. Based on these results, we suggest that TLR4/NF-κB and Wnt/frizzled signaling pathways, as well as estrogen receptors, regulate the progression of endometriosis. These pathways may be therapeutic and diagnostic targets for endometriosis.

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Section: Discussionmentioning

confidence: 99%

Identification and analysis of novel endometriosis biomarkers via integrative bioinformatics

Bae

Cho

et al. 2022

Front. Endocrinol.

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“…conserved parturition mechanism in mammals and live bearing teleost guppies. Notably, OT had opposite effects on luteal phase mares; however, the levels of a series of enzymes involved in PG synthesis, including cox2, were decreased after OT administration (30). The data of dual ISH indicated that itr localization followed a stage-related expression pattern.…”

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confidence: 89%

“…Treatment of cultured endometrial cells with OT induces COX2 activation and synthesis of PGF 2a (29). However, in luteal phase mares, OT downregulated the activities of both COX2 and prostaglandin E 2 synthase (PTGES) (30). Thus, OT functions involve regulation of PG synthesis, which may be influenced by the reproductive status.…”

Section: Introductionmentioning

confidence: 99%

Arg-Vasotocin Directly Activates Isotocin Receptors and Induces COX2 Expression in Ovoviviparous Guppies

Lyu

Wang

et al. 2021

Front. Endocrinol.

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Oxytocin (OT) is a crucial regulator of reproductive behaviors, including parturition in mammals. Arg-vasopressin (AVP) is a nonapeptide homologous to Arg-vasotocin (AVT) in teleosts that has comparable affinity for the OT receptor. In the present study, ovoviviparous guppies (Poecilia reticulata) were used to study the effect of AVT on delivery mediated by the activation of prostaglandin (PG) biosynthesis via isotocin (IT) receptors (ITRs). One copy each of it and avt and two copies of itrs were identified in guppies. The results of the affinity assay showed that various concentrations of AVT and IT (10−6, 10−7, and 10−8 mol/L) significantly activated itr1 (P < 0.05). In vitro experiments revealed significant upregulation (P < 0.05) of cyclooxygenase 2 (cox2), which is the rate-limiting enzyme involved in PG biosynthesis, and itr1 by AVT and IT. Furthermore, dual in situ hybridization detected positive signals for itr1 and cox2 at the same site, implying that ITR1 may regulate cox2 gene expression. Measurement of prostaglandin F2a (PGF2a) concentrations showed that AVT induced PGF2a synthesis (P < 0.05) and that the effect of IT was not significant. Finally, intraperitoneal administration of PGF2a significantly induced premature parturition of guppies. This study is the first to identify and characterize AVT and ITRs in guppies. The findings suggest that AVT promotes PG biosynthesis via ITR and that PGF2a induces delivery behavior in ovoviviparous guppies.

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“…Plasma P4 concentrations were measured in duplicate by a validated solid-phase Radioimmunoassay (RIA), without extraction, using a commercial kit (Coat-A-Count; Diagnostic Product Corporation, Los Angeles, CA, USA), and a Wallac (wizard 1470) counter, as previously described [44]. All samples were run in a single assay.…”

Section: Hormone Assaysmentioning

confidence: 99%

Enzymes Present in Neutrophil Extracellular Traps May Stimulate the Fibrogenic PGF2α Pathway in the Mare Endometrium

Rebordão

Amaral

Fernandes

et al. 2021

Animals

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Endometrosis, a fibrotic disease of mare endometrium, impairs uterine function. Prostaglandins (PG), despite modulating reproductive physiological functions, may also cause local pathological collagen deposition (fibrogenesis). We have previously shown that neutrophil extracellular traps (NETs) may also favor mare endometrosis. The aim of this study was to investigate the effect of enzymes present in NETs on PGF2α-pathway activation. Kenney and Doig’s type I/IIA and IIB/III mare endometria, from follicular phase (FLP) and mid-luteal (MLP) phase, were cultured in vitro in the presence of NETs enzymes (elastase, cathepsin-G or myeloperoxidase). Production of PGF2α (EIA) and transcription (qPCR) of its synthases (PTGS2, AKR1C3) and receptor (PTGFR) genes were evaluated. PGF2α and PTGFR were influenced by endometrial category and estrous cycle phase. In FLP endometrium, NETs enzymes induced both high PGF2α production and/or PTGFR transcription. In MLP type I/IIA tissues, down-regulation of PTGFR transcripts occurred. However, in MLP type IIB/III endometrium, high levels of PTGFR transcripts were induced by NETs enzymes. As PGF2α-pathway activation facilitates fibrogenesis in other tissues, PGF2α may be involved in endometrosis pathogenesis. In the mare, the endocrine microenvironment of healthy and pathological endometrium might modulate the PGF2α pathway, as well as fibrosis outcome on endometrium challenged by NETs enzymes.

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Endometrial prostaglandin synthases, ovarian steroids, and oxytocin receptors in mares with oxytocin-induced luteal maintenance

Cited by 20 publications

References 47 publications

Identification and analysis of novel endometriosis biomarkers via integrative bioinformatics

Identification and analysis of novel endometriosis biomarkers via integrative bioinformatics

Arg-Vasotocin Directly Activates Isotocin Receptors and Induces COX2 Expression in Ovoviviparous Guppies

Enzymes Present in Neutrophil Extracellular Traps May Stimulate the Fibrogenic PGF2α Pathway in the Mare Endometrium

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