Endometrial and conceptus expression of HoxA10, transforming growth factor β1, leukemia inhibitory factor, and prostaglandin H synthase-2 in early pregnant pigs with gonadotropin-induced estrus

Blitek, Agnieszka; Kaczmarek, Monika M.; Kiewisz, Jolanta; Ziȩcik, Adam J.

doi:10.1016/j.domaniend.2009.11.001

Cited by 35 publications

(37 citation statements)

References 37 publications

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“…LIF is involved in controlling the uterine receptivity in other species and is essential for implantation in mice (Paria et al 2002). Interestingly, content of LIF mRNA gradually increases in the porcine endometrium between days 10 and 15 of pregnancy (Blitek et al 2010a). It needs to be determinated if estrogen of conceptus origin can stimulate LIF expression in the pig similarly as ovarian estrogen does in mice and other species.…”

Section: Nk Cellsmentioning

confidence: 99%

“…Although the HOXA10 gene plays a role in uterine development, it is also expressed in adult uteri in many species, including pigs (Blitek et al 2010a). In mice, targeted disruption of HOXA10 expression leads to implantation failure.…”

Section: Uterine Receptivity Markers Involved In Pg Synthesismentioning

confidence: 99%

“…Our recent studies indicate that both PTGS2 and HOXA10 genes may be simultaneously regulated by steroids in the porcine endometrium (Blitek et al 2010b). Endometrial HOXA10 expression is stimulated by E 2 (Blitek et al 2010b) and its mRNA content is increased during implantation in the pig (Blitek et al 2010a). …”

Section: Uterine Receptivity Markers Involved In Pg Synthesismentioning

confidence: 99%

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Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Wacławik

2011

Reproduction

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Ovarian progesterone induces essential changes leading to a temporary state of uterine receptivity for conceptus implantation. Estrogens secreted by the porcine conceptus on days 11 and 12 of pregnancy provide the initial signal for maternal recognition of pregnancy and maintenance of a functional corpus luteum (CL) for continued production of progesterone. As prostaglandins F 2a (PGF 2a ) and E 2 (PGE 2 ) exert opposing actions on the CL, a tight control over their synthesis and secretion is critical either for the initiation of luteolysis or maintenance of pregnancy. One of the supportive mechanisms by which conceptus inhibits luteolysis is changing PG synthesis in favor of luteoprotective PGE 2 . Conceptus PGE 2 could be amplified by PGE 2 feedback loop in the endometrium. In pigs, as in other species, implantation and establishment of pregnancy is associated with upregulation of expression of proinflammatory factors, which include cytokines, growth factors, and lipid mediators. The conceptus produces inflammatory mediators: interferon g and interferon d, interleukins IL1B and IL6, and PGs, which probably activate inflammatory pathways in the endometrium. The endometrium responds to these embryonic signals by enhancing further progesterone-induced uterine receptivity. Understanding the mechanisms of pregnancy establishment is required for translational research to increase reproductive efficiencies and fertility in humans and animals.

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Section: Nk Cellsmentioning

confidence: 99%

Section: Uterine Receptivity Markers Involved In Pg Synthesismentioning

confidence: 99%

Section: Uterine Receptivity Markers Involved In Pg Synthesismentioning

confidence: 99%

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Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Wacławik

2011

Reproduction

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“…This gene has been shown to be expressed in adult murine [9], canine [10], primate [11], human [8, 9], and more recently porcine [12,13] uteri. In pigs, HOXA10 protein was localized in the luminal and glandular epithelium, stroma and blood vessels of the endometrium, but also in the myometrium of the uterus [13]. Mice with targeted mutation of the Hoxa10 gene exhibit implantation failures [14].…”

mentioning

confidence: 99%

“…It was carried out at 25 C for 10 min, 37 C for 120 min and then 85 C for 5 sec. Real-time PCR was performed with an ABI Prism 7300 sequence detection system using Power SYBR Green PCR master mix (Applied Biosystems), as described previously [13]. Each real-time PCR reaction contained 3.5 μl of diluted RT product, 0.5 μM of each forward and reverse primer and 12.5 μl of Power SYBR Green PCR master mix.…”

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confidence: 99%

Effect of Steroids on HOXA10 mRNA and Protein Expression and Prostaglandin Production in the Porcine Endometrium

Blitek

Kiewisz

Wacławik

et al. 2010

Journal of Reproduction and Development

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Abstract. The homeobox A (HOXA) family of genes is responsible for segmental development of the female reproductive tract during embryogenesis. However, HOXA10 has been shown to be essential not only for uterus development, but also for implantation. Persistent expression and steroid-dependent regulation of this gene has been demonstrated in adult human, primate, murine and canine uteri. Moreover, HOXA10-dependent expression of prostaglandin H synthase-2 (PGHS-2), a key enzyme in prostaglandin production, has been previously detected. The role of the HOXA10 gene in the porcine uterus is not well established. Therefore, the present studies were undertaken to 1) examine the effect of E2 and P4 on HOXA10 mRNA and protein content in the endometrium collected on day 9 of the estrous cycle and 2) determine the PGHS-2 protein expression and PGE2 and PGF2α secretion from endometrial tissue in response to steroid treatment. Endometrial explants collected from mature gilts on day 9 of the estrous cycle were incubated with E2 (1-100 nM), P4 (10-1000 nM) or E2 (10 nM) and P4 (100 nM) for 24 h. E2 alone or E2 in the presence of P4 increased HOXA10 mRNA expression in the endometrium (P<0.05). The HOXA10 protein level was upregulated in response to E2, P4 and both steroids administered simultaneously (P<0.05). Moreover, E2 and P4 stimulated PGHS-2 protein expression in cultured endometrial explants. PGE2, but not PGF2α, secretion increased in the presence of E2 (P<0.05). However, the release of both prostaglandins was decreased after treatment of endometrial explants with the highest dose of P4 (P<0.01). These results demonstrate that E2 and P4 are important regulators of HOXA10 gene expression in the adult porcine endometrium during the mid-luteal phase of the estrous cycle. Additionally, the similar profiles of endometrial HOXA10 and PGHS-2 expression in the presence of E2 and P4 indicate that both genes are simultaneously regulated by steroids in the porcine uterus. Key words: Endometrium, Estradiol, HOXA10, PGHS-2, Pig, Progesterone, Prostaglandins (J. Reprod. Dev. 56: [643][644][645][646][647][648] 2010) he uterine endometrium is one of the most dynamic tissues in the organism, undergoing morphological and physiological changes during each estrous cycle. Disorders in endometrial development may lead to lack of or improper implantation, pregnancy failure or infertility. Among the different hormones, estradiol (E2) and progesterone (P4) of ovarian origin are crucial regulators of endometrial receptivity and embryo-maternal cross-talk in many species [1][2][3][4]. It is obvious that the action of P4 in priming the uterus is absolutely essential to attain uterine receptivity for implantation, but the requirement of E2 of ovarian origin is speciesspecific [1]. However, the molecular mechanisms that direct the ordered proliferation, differentiation and cell death associated with the normal menstrual or estrous cycles are still not well characterized. Good candidates for regulation of gene expression in the endometrium are ho...

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Novel insights into conceptus–maternal signaling during pregnancy establishment in pigs

Kaczyński

Goryszewska-Szczurek

Baryla

et al. 2022

Molecular Reproduction Devel

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Pregnancy establishment in mammals, including pigs, requires coordinated communication between developing conceptuses (embryos with associated membranes) and the maternal organism. Porcine conceptuses signalize their presence by secreting multiple factors, of which estradiol‐17β (E2) is considered the major embryonic signal initiating the maternal recognition of pregnancy. During this time, a limited supply of prostaglandin (PGF2α) to the corpora lutea and an increased secretion of luteoprotective factors (e.g., E2 and prostaglandin E2 [PGE2]) lead to the corpus luteum's maintained function of secreting progesterone, which in turn primes the uterus for implantation. Further, embryo implantation is related to establishing an appropriate proinflammatory environment coordinated by the secretion of proinflammatory mediators including cytokines, growth factors, and lipid mediators of both endometrial and conceptus origin. The novel, dual role of PGF2α has been underlined. Recent studies involving high‐throughput technologies and sophisticated experimental models identified a number of novel factors and revealed complex relationships between these factors and those already established. Hence, it seems that early pregnancy should be regarded as a sequence of processes orchestrated by pleiotropic factors that are involved in redundancy and compensatory mechanisms that preserve the essential functions critical for implantation and placenta formation. Therefore, establishing the hierarchy between all molecules present at the embryo–maternal interface is now even more challenging

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Endometrial and conceptus expression of HoxA10, transforming growth factor β1, leukemia inhibitory factor, and prostaglandin H synthase-2 in early pregnant pigs with gonadotropin-induced estrus

Cited by 35 publications

References 37 publications

Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Effect of Steroids on HOXA10 mRNA and Protein Expression and Prostaglandin Production in the Porcine Endometrium

Novel insights into conceptus–maternal signaling during pregnancy establishment in pigs

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