Endomembrane remodeling and dynamics in Salmonella infection

Fang, Ziyan; Méresse, Stéphane

doi:10.15698/mic2022.02.769

Cited by 10 publications

(10 citation statements)

References 147 publications

(183 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein is important in intracellular transports, the mRNA surveillance pathway, and adaptive immune response. KLC1 was shown to be active in viral, e.g., poxvirus [ 63 , 64 ], and bacterial infections, e.g., Salmonella [ 65 ], during the host-pathogen interactions [ 64 ]. It is documented that KLC1 participates in MHC-II antigen processing and presentation via microtubule motility [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

First Steps towards the Development of Epigenetic Biomarkers in Female Cheetahs (Acinonyx jubatus)

Weyrich

Guerrero-Altamirano

Yasar

et al. 2022

Life

View full text Add to dashboard Cite

Free-ranging cheetahs (Acinonyx jubatus) are generally healthy, whereas cheetahs under human care, such as those in zoological gardens, suffer from ill-defined infectious and chronic pathologies. These differences are only partially explained by husbandry management programs because both groups share low genetic diversity. However, mounting evidence suggests that physiological differences between populations in different environments can be tracked down to differences in epigenetic signatures. Here, we identified differentially methylated regions (DMRs) between free-ranging cheetahs and conspecifics in zoological gardens and prospect putative links to pathways relevant to immunity, energy balance and homeostasis. Comparing epigenomic DNA methylation profiles obtained from peripheral blood mononuclear cells (PBMCs) from eight free-ranging female cheetahs from Namibia and seven female cheetahs living in zoological gardens within Europe, we identified DMRs of which 22 were hypermethylated and 23 hypomethylated. Hypermethylated regions in cheetahs under human care were located in the promoter region of a gene involved in host-pathogen interactions (KLC1) and in an intron of a transcription factor relevant for the development of pancreatic β-cells, liver, and kidney (GLIS3). The most canonical mechanism of DNA methylation in promoter regions is assumed to repress gene transcription. Taken together, this could indicate that hypermethylation at the promoter region of KLC1 is involved in the reduced immune competence in cheetahs under human care. This approach can be generalized to characterize DNA methylation profiles in larger cheetah populations under human care with a more granular longitudinal data collection, which, in the future, could be used to monitor the early onset of pathologies, and ultimately translate into the development of biomarkers with prophylactic and/or therapeutic potential.

show abstract

Section: Discussionmentioning

confidence: 99%

First Steps towards the Development of Epigenetic Biomarkers in Female Cheetahs (Acinonyx jubatus)

Weyrich

Guerrero-Altamirano

Yasar

et al. 2022

Life

View full text Add to dashboard Cite

show abstract

“…In the case of bacteria that cause system infections like L. monocytogenes , entry also allows traversal of anatomical barriers, such as the intestinal, foetal‐placental and blood–brain barriers (Kammoun et al, 2022). After internalization into human cells, bacteria are encased in membranous vacuoles derived from the plasma membrane (Fang & Méresse, 2022; Pizarro‐Cerdá et al, 2016; Sherwood & Roy, 2016). While S. enterica replicates within vacuoles, L. monocytogenes and S. flexneri escape from these membrane compartments to gain access to the host cytosol (Pizarro‐Cerda et al, 2016).…”

Section: Exploitation Of the Host Exocyst By Bacteriamentioning

confidence: 99%

Exploitation of the host exocyst complex by bacterial pathogens

Ireton

Gyanwali

Herath

et al. 2023

Molecular Microbiology

View full text Add to dashboard Cite

Intracellular bacterial pathogens remodel the plasma membrane of eukaryotic cells in order to establish infection. A common and well-studied mechanism of plasma membrane remodelling involves bacterial stimulation of polymerization of the host actin cytoskeleton. Here, we discuss recent results showing that several bacterial pathogens also exploit the host vesicular trafficking pathway of 'polarized exocytosis' to expand and reshape specific regions in the plasma membrane during infection.Polarized exocytosis is mediated by an evolutionarily conserved octameric protein complex termed the exocyst. We describe examples in which the bacteria Listeria monocytogenes, Salmonella enterica serovar Typhimurium, and Shigella flexneri co-opt the exocyst to promote internalization into human cells or intercellular spread within host tissues. We also discuss results showing that Legionella pneumophila or S. flexneri manipulate exocyst components to modify membrane vacuoles to favour intracellular replication or motility of bacteria. Finally, we propose potential ways that pathogens manipulate exocyst function, discuss how polarized exocytosis might promote infection and highlight the importance of future studies to determine how actin polymerization and polarized exocytosis are coordinated to achieve optimal bacterial infection.

show abstract

“…A subsets of SPI2-T3SS effectors is recruited to Salmonella -modified membranes (SMM) after translocation that is prominently associated with membranes of the SCV and SIF network 12 . This subcellular localization appears to be prerequisite for effector and host protein interactions that mediate vesicle fusion and SIF elongation 13 . However, the molecular mechanisms of effector targeting to SMM are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Single molecule analyses reveal dynamics of Salmonella translocated effector proteins in host cell endomembranes

et al. 2023

View full text Add to dashboard Cite

The facultative intracellular pathogen Salmonella enterica remodels the host endosomal system for survival and proliferation inside host cells. Salmonella resides within the Salmonella-containing vacuole (SCV) and by Salmonella-induced fusions of host endomembranes, the SCV is connected with extensive tubular structures termed Salmonella-induced filaments (SIF). The intracellular lifestyle of Salmonella critically depends on effector proteins translocated into host cells. A subset of effectors is associated with, or integral in SCV and SIF membranes. How effectors reach their subcellular destination, and how they interact with endomembranes remodeled by Salmonella remains to be determined. We deployed self-labeling enzyme tags to label translocated effectors in living host cells, and analyzed their single molecule dynamics. Translocated effectors diffuse in membranes of SIF with mobility comparable to membrane-integral host proteins in endomembranes. Dynamics differ between various effectors investigated and is dependent on membrane architecture of SIF. In the early infection, host endosomal vesicles are associated with Salmonella effectors. Effector-positive vesicles continuously fuse with SCV and SIF membranes, providing a route of effector delivery by translocation, interaction with endosomal vesicles, and ultimately fusion with the continuum of SCV/SIF membranes. This mechanism controls membrane deformation and vesicular fusion to generate the specific intracellular niche for bacterial survival and proliferation.

show abstract

Endomembrane remodeling and dynamics in Salmonella infection

Cited by 10 publications

References 147 publications

First Steps towards the Development of Epigenetic Biomarkers in Female Cheetahs (Acinonyx jubatus)

First Steps towards the Development of Epigenetic Biomarkers in Female Cheetahs (Acinonyx jubatus)

Exploitation of the host exocyst complex by bacterial pathogens

Single molecule analyses reveal dynamics of Salmonella translocated effector proteins in host cell endomembranes

Contact Info

Product

Resources

About