Endoglucanase and xylanase production byBacillussp. AR03 in co-culture

Abstract

The behavior of three isolates retrieved from different cellulolytic consortia, Bacillus sp. AR03, Paenibacillus sp. AR247 and Achromobacter sp. AR476-2, were examined individually and as co-cultures in order to evaluate their ability to produce extracellular cellulases and xylanases. Utilizing a peptone-based medium supplemented with carboxymethyl cellulose (CMC), an increase estimation of 1.30 and 1.50 times was obtained by the co-culture containing the strains AR03 and AR247, with respect to enzyme titles registered by their individual cultivation. On the contrary, the extracellular enzymatic production decreased during the co-cultivation of strain AR03 with the non-cellulolytic Achromobacter sp. AR476-2. The synergistic behavior observed through the combined cultivation of the strains AR03 and AR247 might be a consequence of the consumption by Paenibacillus sp. AR247 of the products of the CMC hydrolysis (i.e., cellobiose and/or cello-oligosaccharides), which were mostly generated by the cellulase producer Bacillus sp. AR03. The effect observed could be driven by the requirement to fulfill the nutritional supply from both strains on the substrate evaluated. These results would contribute to a better description of the degradation of the cellulose fraction of the plant cell walls in nature, expected to an efficient utilization of renewable sources.

Effect of Fortified Daqu on the Microbial Community and Flavor in Chinese Strong-Flavor Liquor Brewing Process

“…For example, Bacillus licheniformis could produce aromatic compounds, C4 compounds, pyrazines, and volatile acids by solid-state fermentation (Zhang et al, 2013). More importantly, improvement of hydrolase and flavor precursor production was obtained when Bacillus was co-cultured (Hero et al, 2017; Samad and Zainol, 2017). The corresponding liquor L50-M also exhibited higher contents of aromatic compounds, including 2,4-di-ter-butyl-phenol, (2,2-diethoxyethyl)-benzene, benzeneethanol, and ethyl phenylacetate (Supplementary Table S3).…”

Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF

“…The absorbance was measured at 540 nm. One unit of xylanase activity was defined as the liberation of reducing sugars equivalent to 1 μmol of xylose/ min under the assay condition estimated according to the previously described DNS method (Hero et al 2017).…”

Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

“…On the other hand, co-cultivation of microorganisms had extensively used to solve the practical obstructions related to axenic cultivation. Co-cultivation of microorganism has been effectively used to convert the complex substrates into the simple biomolecules for the industrial applications [15][16][17][18]. In recent times, the genetically engineered microbial strains are used in co-cultivation to enhance the speci c metabolites through metabolic pathways [19].…”