Endogenous tumor necrosis factor, interleukin-6, and gamma interferon levels during Listeria monocytogenes infection in mice

Abstract

Mice were infected intravenously with a sublethal dose of Listeria monocytogenes cells and then levels of tumor necrosis factor (TNF), interleukin-6 (IL-6), and gamma interferon (IFN-y) in the bloodstreams, spleens, and livers were monitored. The maximum level of TNF was detected at 72 h in the spleens and livers, but TNF was never detected in the bloodstreams. IL-6 appeared in the bloodstreams and spleens and peaked at 48 h. The maximum level of IFN-y could be detected in all three specimens, and the highest titer was shown in the spleens. Endogenous TNF production was suppressed by in vivo administration of anti-CD4 monoclonal antibody (MAb) or anti-asialo GM1 antibody but not by anti-CD8 MAb, whereas none of these antibodies suppressed endogenous IL-6 production. Endogenous production of neither IL-6 nor IFN-y was inhibited in rabbit antirecombinant mouse TNF-a antibody-treated mice. Similarly, production of TNF and IL-6 did not decrease in anti-mouse IFN-y MAb-treated animals, but TNF production was augmented in these animals. These results suggest that the these endogenous cytokines are produced by different mechanisms in L. monocytogenes infection. Complete elimination of Listeria monocytogenes, a facul-523 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from INFECT. IMMUN. on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

Regulation of lipopolysaccharide-induced tumor necrosis factor production by cyclosporin A in mice primed with muramyl dipeptide

“…Thus, the decrease in TNF levels was not correlated with an inhibitory effect on IL-6 production, at least at the 1.5 h time point. At the peak of the response (at 2.5 h) a strong inhibition in the TNF yield by CsA produced a slight decrease in the IL-6 titer, namely in normal or galactosamine-treated mice ( Table 2) confirming that IL-6 production can be dissociated from TNF levels in the blood as already reported under various experimental conditions [20,27,28]. In contrast, in adrenalectomized mice, CsA injection produced a marked inhibition of serum IL-6 level early after the LPS challenge indicating a differential sensitivity to CsA of IL-6-producing cells in the absence of endogenous glucocorticoids.…”

Malaria, blood glucose, and the role of tumour necrosis factor (TNF) in mice

“…Preparation of spleen homogenates [27] Spleens that had been stored at ÿ 208C were disrupted in a glass homogenizer to give 20% suspensions in ice-cold Earle's balanced salt solution (BSS; GIBCO Life Technologies Ltd, Paisley, UK) containing 1% (w/v) 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate (CHAPS; Sigma). Suspensions were left overnight on ice, clarified by centrifugation in a microfuge and assayed for TNF.…”

Neuropeptides in the livers of mice during bacterial infections

“…We also investigated the detection of VIP and SP in the livers of T. paurometabolum-injected mice. Our previous study [29] showed that the granulomas developed in the liver, spleen and lung until 3 weeks after injection, and thereafter regress in these ani- mals. Therefore, mice were injected intravenously with 10 V cfu of viable T. paurometabolum in saline, and formalin-¢xed para¤n-embedded sections of the livers were obtained from the mice on day 21 of injection.…”