Endogenous Transcription Occurs at the 1-Cell Stage in the Mouse Embryo

Bouniol, Christine; Nguyen, Éric; Debey, Pascale

doi:10.1006/excr.1995.1130

Cited by 276 publications

(215 citation statements)

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“…We also validated the BrUTP antibody specificity by substituting BrUTP with UTP ( Figure 4A). Consistent with previous reports [27,28], global transcriptional activity of the paternal genome, which is marked by lack of H3K9me2 staining, is significantly higher than that of the maternal genome ( Figure 4A). …”

Section: Tet3-mediated 5mc Oxidation Is Dispensable For Zygotic Gene supporting

confidence: 93%

“…RT-qPCR analysis confirmed that the expression dynamics of major satellite DNA and Tet3 previously shown to be unregulated at the 1-cell stage are consistent with previous results [10,29]. This result indicates that some TEs are activated in the 1-cell embryos when global transcription is also initiated [27,28]. The asymmetric nature of 5mC oxidation in zygotes prompted us to ask whether activation of the TEs in zygotes exhibits allele specificity.…”

Section: Te Expression From Both Paternal and Maternal Alleles In 1-csupporting

confidence: 86%

“…Zygotic gene activation, the earliest expression from zygote genome after fertilization, begins at the middle of 1-cell stage [27,28]. To test whether Tet3-mediated 5mC oxidation is involved in zygotic gene activation, we analyzed de novo RNA synthesis by measuring incorporation of 5-bromouridine-5′-triphosphate (BrUTP).…”

Section: Tet3-mediated 5mc Oxidation Is Dispensable For Zygotic Gene mentioning

confidence: 99%

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Transcriptional activation of transposable elements in mouse zygotes is independent of Tet3-mediated 5-methylcytosine oxidation

2012

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The methylation state of the paternal genome is rapidly reprogrammed shortly after fertilization. Recent studies have revealed that loss of 5-methylcytosine (5mC) in zygotes correlates with appearance of 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). This process is mediated by Tet3 and the 5mC oxidation products generated in zygotes are gradually lost during preimplantation development through a replicationdependent dilution process. Despite these findings, the biological significance of Tet3-mediated oxidation of 5mC to 5hmC/5fC/5caC in zygotes is unknown. DNA methylation plays an important role in silencing gene expression including the repression of transposable elements (TEs). Given that the activation of TEs during preimplantation development correlates with loss of DNA methylation, it is believed that paternal DNA demethylation may have an important role in TE activation. Here we examined this hypothesis and found that Tet3-mediated 5mC oxidation does not have a significant contribution to TE activation. We show that the expression of LINE-1 (long interspersed nucleotide element 1) and ERVL (endogenous retroviruses class III) are activated from both paternal and maternal genomes in zygotes. Inhibition of 5mC oxidation by siRNA-mediated depletion of Tet3 affected neither TE activation, nor global transcription in zygotes. Thus, our study provides the first evidence demonstrating that activation of both TEs and global transcription in zygotes are independent of Tet3-mediated 5mC oxidation.

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Section: Tet3-mediated 5mc Oxidation Is Dispensable For Zygotic Gene supporting

confidence: 93%

Section: Te Expression From Both Paternal and Maternal Alleles In 1-csupporting

confidence: 86%

Section: Tet3-mediated 5mc Oxidation Is Dispensable For Zygotic Gene mentioning

confidence: 99%

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Transcriptional activation of transposable elements in mouse zygotes is independent of Tet3-mediated 5-methylcytosine oxidation

2012

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“…During pronuclear stages, these maternally derived components degrade rapidly, and transcription of the zygotic genome has to be initiated for subsequent embryonic development [10,11]. Gene transcription during the transition from maternal factors to those of the zygote is called zygotic gene activation (ZGA), which consists of two waves: the late pronuclear stage (minor ZGA) followed by the two-cell stage (major ZGA) [12,13]. Minor ZGA is the first activation of transcription from the zygotic genome and occurs in the middle pronuclear stages.…”

Section: Introductionmentioning

confidence: 99%

Paternal H3K4 methylation is required for minor zygotic gene activation and early mouse embryonic development

et al. 2015

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Epigenetic modifications, such as DNA methylation and histone modifications, are dynamically altered predominantly in paternal pronuclei soon after fertilization. To identify which histone modifications are required for early embryonic development, we utilized histone K-M mutants, which prevent endogenous histone methylation at the mutated site. We prepared four single K-M mutants for histone H3.3, K4M, K9M, K27M, and K36M, and demonstrate that overexpression of H3.3 K4M in embryos before fertilization results in developmental arrest, whereas overexpression after fertilization does not affect the development. Furthermore, loss of H3K4 methylation decreases the level of minor zygotic gene activation (ZGA) predominantly in the paternal pronucleus, and we obtained similar results from knockdown of the H3K4 methyltransferase Mll3/4. We therefore conclude that H3K4 methylation, likely established by Mll3/4 at the early pronuclear stage, is essential for the onset of minor ZGA in the paternal pronucleus, which is necessary for subsequent preimplantation development in mice.

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“…As an alternative to the autoradiographic approach, a non-isotopic immunocytochemical method of RNA synthesis detection can also be used [4,7,19,20,27,32,39]. It [19,20,31,39].…”

mentioning

confidence: 99%

Non-isotopic detection of nucleolar transcription in pre-implantation mouse embryos

Kobema

Landa²,

Kaňka³

et al. 1998

Reprod. Nutr. Dev.

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Endogenous Transcription Occurs at the 1-Cell Stage in the Mouse Embryo

Cited by 276 publications

References 0 publications

Transcriptional activation of transposable elements in mouse zygotes is independent of Tet3-mediated 5-methylcytosine oxidation

Transcriptional activation of transposable elements in mouse zygotes is independent of Tet3-mediated 5-methylcytosine oxidation

Paternal H3K4 methylation is required for minor zygotic gene activation and early mouse embryonic development

Non-isotopic detection of nucleolar transcription in pre-implantation mouse embryos

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