Abstract:1 Exogenous and endogenous tachykinins facilitate cholinergic nerve-induced bronchoconstriction in guinea-pig. Using a vagally innervated guinea-pig tracheal tube preparation we have investigated the involvement of endogenous capsaicin-sensitive neuropeptides in both pre-and postganglionic cholinergic neurotransmission. The effects of the neutral endopeptidase inhibitor (NEP), phosphoramidon, were investigated in this preparation either alone or in conjunction with sensory neuropeptide depletion by capsaicin p… Show more
“…Thus the logical explanation of the IL-1 effect is that SP alters cholinergic responsiveness. Previous studies have shown that SP enhances cholinergic responsiveness either through a direct effect on airway smooth muscle (49) or by enhancing ACh release from parasympathetic nerve terminals (40,44,52). The data in the present study do not discriminate between these two mechanisms because cholinergic responsiveness was enhanced both by cholinergic agonists and by EFS.…”
Dey. Interleukin-1-induced airway hyperresponsiveness enhances substance P in intrinsic neurons of ferret airway. Am J Physiol Lung Cell Mol Physiol 283: L909-L917, 2002. First published April 12, 2002 10.1152/ajplung.00363.2001.-Interleukin (IL)-1 causes airway inflammation, enhances airway smooth muscle responsiveness, and alters neurotransmitter expression in sensory, sympathetic, and myenteric neurons. This study examines the role of intrinsic airway neurons in airway hyperresponsiveness (AHR) induced by IL-1. Ferrets were instilled intratracheally with IL-1 (0.3 g/0.3 ml) or saline (0.3 ml) once daily for 5 days. Tracheal smooth muscle contractility in vitro and substance P (SP) expression in tracheal neurons were assessed. Tracheal smooth muscle reactivity to acetylcholine (ACh) and methacholine (MCh) and smooth muscle contractions to electric field stimulation (EFS) both increased after IL-1. The IL-1-induced AHR was maintained in tracheal segments cultured for 24 h, a procedure that depletes SP from sensory nerves while maintaining viability of intrinsic airway neurons. Pretreatment with CP-99994, an antagonist of neurokinin 1 receptor, attenuated the IL-1-induced hyperreactivity to ACh and MCh and to EFS in cultured tracheal segments. SP-containing neurons in longitudinal trunk, SP innervation of superficial muscular plexus neurons, and SP nerve fiber density in tracheal smooth muscle all increased after treatment with IL-1. These results show that IL-1-enhanced cholinergic airway smooth muscle contractile responses are mediated by the actions of SP released from intrinsic airway neurons. airway inflammation; airway smooth muscle contraction; muscarinic agonists; neurokinin receptor; airway innervation
“…Thus the logical explanation of the IL-1 effect is that SP alters cholinergic responsiveness. Previous studies have shown that SP enhances cholinergic responsiveness either through a direct effect on airway smooth muscle (49) or by enhancing ACh release from parasympathetic nerve terminals (40,44,52). The data in the present study do not discriminate between these two mechanisms because cholinergic responsiveness was enhanced both by cholinergic agonists and by EFS.…”
Dey. Interleukin-1-induced airway hyperresponsiveness enhances substance P in intrinsic neurons of ferret airway. Am J Physiol Lung Cell Mol Physiol 283: L909-L917, 2002. First published April 12, 2002 10.1152/ajplung.00363.2001.-Interleukin (IL)-1 causes airway inflammation, enhances airway smooth muscle responsiveness, and alters neurotransmitter expression in sensory, sympathetic, and myenteric neurons. This study examines the role of intrinsic airway neurons in airway hyperresponsiveness (AHR) induced by IL-1. Ferrets were instilled intratracheally with IL-1 (0.3 g/0.3 ml) or saline (0.3 ml) once daily for 5 days. Tracheal smooth muscle contractility in vitro and substance P (SP) expression in tracheal neurons were assessed. Tracheal smooth muscle reactivity to acetylcholine (ACh) and methacholine (MCh) and smooth muscle contractions to electric field stimulation (EFS) both increased after IL-1. The IL-1-induced AHR was maintained in tracheal segments cultured for 24 h, a procedure that depletes SP from sensory nerves while maintaining viability of intrinsic airway neurons. Pretreatment with CP-99994, an antagonist of neurokinin 1 receptor, attenuated the IL-1-induced hyperreactivity to ACh and MCh and to EFS in cultured tracheal segments. SP-containing neurons in longitudinal trunk, SP innervation of superficial muscular plexus neurons, and SP nerve fiber density in tracheal smooth muscle all increased after treatment with IL-1. These results show that IL-1-enhanced cholinergic airway smooth muscle contractile responses are mediated by the actions of SP released from intrinsic airway neurons. airway inflammation; airway smooth muscle contraction; muscarinic agonists; neurokinin receptor; airway innervation
“…However, in rabbit trachea the tachykinin receptors mediating this effect were not investigated. Exogenous tachykinins also potentiate cholinergic neurotransmission at pre-and postganglionic nerve terminals in guinea-pig trachea (Hall et al, 1989;Watson et al, 1993). The tachykinin receptor mediating these effects appeared to be of the NK, receptor subtype (Watson et al, 1993).…”
Section: Discussionmentioning
confidence: 91%
“…Exogenous tachykinins also potentiate cholinergic neurotransmission at pre-and postganglionic nerve terminals in guinea-pig trachea (Hall et al, 1989;Watson et al, 1993). The tachykinin receptor mediating these effects appeared to be of the NK, receptor subtype (Watson et al, 1993). However, in addition, NKA, which preferentially stimulates NK2 receptors facilitated contractions evoked by pre-and postganglionic nerve stimulation (Hall et al, 1989;Watson et al, 1993).…”
Section: Discussionmentioning
confidence: 98%
“…In addition, SP potentiates cholinergic nerve-induced contractions in rabbit trachea in vitro via a postganglionic, prejunctional mechanism (Armour et al, 1991). Exogenous tachykinins also potentiate cholinergic neurotransmission in guinea-pig trachea (Hall et al, 1989;Watson et al, 1993).…”
Section: Introductionmentioning
confidence: 99%
“…Capsaicin, at a sub-threshold concentration, acutely releases tachykinins which enhance cholinergic neurotransmission in guinea-pig trachea in vitro (Aizawa et al, 1990). In addition, the inhibitor of neutral endopeptidase (NEP), phosphoramidon, increased the amplitude of contractions produced by preganglionic vagal stimulation in guinea-pig trachea and this effect is blocked by capsaicin pretreatment (Watson et al, 1993). However, Aizawa et al (1990) reported that phosphoramidon also enhanced contractions to electrical field stimulation (EFS) in guinea-pig trachea without changing responses to exogenous ACh.…”
1 Exogenous tachykinins modulate cholinergic neurotransmission in rabbit and guinea-pig airways. We have investigated the effect of selective tachykinin receptor agonists and antagonists on cholinergic neurotransmission evoked by electrical field stimulation (EFS) of bronchial rings in rabbit, guinea-pig and human airways in vitro to assess which type of tachykinin receptor is mediating this facilitatory effect. 2 Bronchial rings were set up for isometric tension recording. Contractile responses to EFS (60 V, 0.4 ms, 2 Hz for lOs every min) and exogenous acetylcholine (ACh) were obtained and the effects of selective tachykinin agonists and antagonists were investigated. 3 In rabbit bronchi the endogenous tachykinins, substance P (SP) and neurokinin A (NKA) (10 nM) potentiated cholinergic responses to EFS (by 287.6 ± 121%, P < 0.01 and 181.4 ± 56.5%, P < 0.001 respectively).4 The NK1 receptor selective agonist, [SarISP sulphone (10 nM) evoked a maximal facilitatory action on cholinergic responses of 334.9 ± 63% (P<0.01) (pD2 = 8.5 ± 0.06) an effect which was blocked by the selective NKI-receptor antagonist, CP 96,345 (100 nM) (P <0.05) but not by the NK2 receptor antagonist, MEN 10,376 (100 nM). The NK2 receptor selective agonist, [PAla8]NKA(4-10) (1O nM), produced a maximum enhancement of 278 ± 83.5% (P<0.01) (pD2 = 8.7 ± 0.1) an effect which was blocked by MEN 10,376 (100 nM) (P <0. 7 By contrast, in guinea-pig bronchi only the NK,-receptor agonist [SarISP sulphone (3 nM) was effective in enhancing cholinergic neurotransmission but the effect was relatively small (maximal enhancement 25.7 ± 5.5%, P< 0.01). In human bronchial rings all the selective neurokinin agonists were without effect on cholinergic neurotransmission. 8 These results suggest that tachykinins may play an important role in modulating cholinergic neurotransmission in rabbit (via NKI and NK2 receptors) and guinea-pig airways (via NKI receptor) but have no demonstrable effect on human airways
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