Endogenous signalling control of cell adhesion by using aptamer functionalized biocompatible hydrogel

Li, Wen; Wang, Jiasi; Ren, Jinsong; Qu, Xiaogang

doi:10.1039/c5sc02565f

Cited by 31 publications

(32 citation statements)

References 63 publications

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“…[153,154] By immobilizing ATP-binding aptamers on the surface of an alginate hydrogel substrate, ATPprogrammed cell adhesion on the hydrogel surface was investigated (Figure 11a). [155] To this end, the aptamer-functionalized hydrogel was designed to bind with a RGD-modified complementary DNA (an anchor for binding to the cell integrins) by simple hybridization, leading to cell adhesion on the surface. When adding ATP to the system, the aptamer however changed partners and instead bound with ATP while dissociating from its complementary RGD-appended ssDNA.…”

Section: Atp Regenerationmentioning

confidence: 99%

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ATP‐Responsive and ATP‐Fueled Self‐Assembling Systems and Materials

2020

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his/her body weight in ATP each day, which is enabled through a very efficient ADP/ATP recycling system. [7] Aside of the fascinating non-equilibrium succeeded by exploiting ATP/GTP as chemical fuels to drive structures and functions, it is also important to realize that ATP and other nucleoside phosphates can be used as biological and chemical signals. [8-10] Lots of biological processes such as chromatin remodeler activation, [11] inflammatory signaling, [12] and cell differentiation [13] are mediated by ATP signaling. Additionally, cancer cells have a higher concentration of ATP, [14] which in turn provides a handle to develop new types of molecular therapies. This of course requires to develop sophisticated carriers or self-assembling structures that highly specifically react to ATP and potentially even to its concentration, ideally without any interference from other nucleoside phosphates. [15-20] For such cases, it is also important to realize that ATP is used as a chemical signal rather than a chemical fuel, as the primary objective may not be to harvest its energy from hydrolysis for functions, but just to use its chemical structure for a change of function. Overall, it becomes clear that the use of ATP (and similarly GTP) as a trigger or as a chemical fuel is of high relevance to interact with living systems and also to be able to create active devices in long term that can create work and allow for active communication in a biological environment using the fuels available therein. [21] In the scope of this review on ATP-dependent systems, and being set at the interface of near-equilibrium responsive materials versus non-equilibrium active materials, it is useful to begin Adenosine triphosphate (ATP) is a central metabolite that plays an indispensable role in various cellular processes, from energy supply to cell-tocell signaling. Nature has developed sophisticated strategies to use the energy stored in ATP for many metabolic and non-equilibrium processes, and to sense and bind ATP for biological signaling. The variations in the ATP concentrations from one organelle to another, from extracellular to intracellular environments, and from normal cells to cancer cells are one motivation for designing ATPtriggered and ATP-fueled systems and materials, because they show great potential for applications in biological systems by using ATP as a trigger or chemical fuel. Over the last decade, ATP has been emerging as an attractive co-assembling component for man-made stimuli-responsive as well as for fuel-driven active systems and materials. Herein, current advances and emerging concepts for ATP-triggered and ATP-fueled self-assemblies and materials are discussed, shedding light on applications and highlighting future developments. By bringing together concepts of different domains, that is from supramolecular chemistry to DNA nanoscience, from equilibrium to nonequilibrium self-assembly, and from fundamental sciences to applications, the aim is to cross-fertilize current approaches with the ultimate aim to bring ...

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Section: Atp Regenerationmentioning

confidence: 99%

“…As already discussed above, ATP-responsive systems can be achieved by either molecular recognition of ATP in co-assemblies or by enzymatic energy transfer. To convert the assembled [155] Copyright 2015, RSC. d-g) Reproduced with permission.…”

Section: Atp-responsive and Reversibly Self-assembling Systems And Mamentioning

confidence: 99%

ATP‐Responsive and ATP‐Fueled Self‐Assembling Systems and Materials

2020

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“…This ATP then bound the aptamer, causing the release of the RGD-containing sequence and leading to a fast detachment of adhered cells (reaching 57% within 20 min). [180] The versatility of aptamers has also been explored beyond biomaterial functionalization to design functional mimetics of GFs, i.e., as agonists to GF receptors. This strategy implies the replacement of expensive and labile proteins with better alternatives that activate the same pathways and cell responses.…”

Section: Selective Ligands For Gf Capturementioning

confidence: 99%

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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“…Upon introduction of ATP (bottom, exogenous or cell-secreted), aptamers release their complementary strand and bind to ATP, thus creating a non-adhesive environment. 193 Aptamer-target interactions can be designed for virtually any target, making this a highly promising, though costly, strategy. (c) was reprinted with permission.…”

Section: Molecule Specificmentioning

confidence: 99%

“…The system was demonstrated to be highly selective to ATP and even responsive to ATP signaling from cells in co-culture. 193 Such a strategy requires non-trivial aptamer generation, but can be applied to a wide variety of targets and incorporated in a wide variety of systems.…”

Section: Molecule Specificmentioning

confidence: 99%

Hydrogels that listen to cells: a review of cell-responsive strategies in biomaterial design for tissue regeneration

et al. 2017

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Endogenous signalling control of cell adhesion by using aptamer functionalized biocompatible hydrogel

Abstract: A dynamic hydrogel was fabricated in which the cell adhesion state could be programmed with signaling molecules in a cellular microenvironment.

Cited by 31 publications

References 63 publications

ATP‐Responsive and ATP‐Fueled Self‐Assembling Systems and Materials

ATP‐Responsive and ATP‐Fueled Self‐Assembling Systems and Materials

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Hydrogels that listen to cells: a review of cell-responsive strategies in biomaterial design for tissue regeneration

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