Endogenous Retrovirus 3 – History, Physiology, and Pathology

Rivera, Yomara Y. Bustamante; Brütting, Christine; Schmidt, Caroline; Volkmer, Ines; Staege, Martin S.

doi:10.3389/fmicb.2017.02691

Cited by 20 publications

(27 citation statements)

References 108 publications

(117 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inhibition of methyltransferases is able to activate ERV expression in cancer cells with subsequent induction of anti-viral pathways [29]. This so-called “viral mimicry” can be considered as a natural defense mechanism against transformed or virus-infected cells [30]. A binary switch model has been proposed for histone regulation [31].…”

Section: Resultsmentioning

confidence: 99%

Expression of A New Endogenous Retrovirus-Associated Transcript in Hodgkin Lymphoma Cells

Schneider

Volkmer

Engel

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

During characterization of a cDNA library from the Hodgkin lymphoma (HL) cell line L-1236, we discovered a new transcript derived from chromosome 1 at the long intergenic non-protein coding RNA 1768 (LINC01768)/colony stimulating factor 1 (CSF1) region. The first exon of this transcript from Hodgkin lymphoma cells (THOLE) starts in the predicted exon 4 of LINC01768 and is part of an endogenous retrovirus (ERV) from the HUERS-P1/LTR8 family. High expression of THOLE was only detectable in HL cell line L-1236. The expression of THOLE in L-1236 cell is another example for ERV/LTR-associated gene expression in HL cells. At the genome level, the HUERS-P1/LTR8 region including THOLE is only present in Hominoidea. The influence of ERV/LTRs on gene expression might explain the characteristic phenotype of human HL.

show abstract

Section: Resultsmentioning

confidence: 99%

Expression of A New Endogenous Retrovirus-Associated Transcript in Hodgkin Lymphoma Cells

Schneider

Volkmer

Engel

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The role of ERV3-1 in cancer appears to be different in several tumor entities. The overexpression of ERV3-1 is associated with several tumor entities like prostate, lung, liver and colorectal cancer ( 8 , 64 , 70 ). However, in the myelogenous leukemia cell line U-937 ERV3 was found to be upregulated during monocyte differentiation ( 71 ) because of demethylation of the ERV3 locus ( 72 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Differentially Expressed Human Endogenous Retrovirus Families in Human Leukemia and Lymphoma Cell Lines and Stem Cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

Endogenous retroviruses (ERVs) are becoming more and more relevant in cancer research and might be potential targets. The oncogenic potential of human ERVs (HERVs) has been recognized and includes immunosuppression, cell fusion, antigenicity of viral proteins, and regulation of neighboring genes. To decipher the role of HERVs in human cancers, we used a bioinformatics approach and analyzed RNA sequencing data from the LL-100 panel, covering 22 entities of hematopoietic neoplasias including T cell, B cell and myeloid malignancies. We compared HERV expression in this panel with hematopoietic stem cells (HSCs), embryonic stem cells (ESCs) and normal blood cells. RNA sequencing data were mapped against a comprehensive synthetic viral metagenome with 116 HERV sequences from 14 different HERV families. Of these, 13 HERV families and elements were differently expressed in malignant hematopoietic cells and stem cells. We found transcriptional upregulation of HERVE family in acute megakaryocytic and erythroid leukemia and of HERVFc family in multiple myeloma/plasma cell leukemia (PCL). The HERVFc member HERVFc-1 was found transcriptionally active in the multiple myeloma cell line OPM-2 and also in the Hodgkin lymphoma cell line L-428. The expression of HERVFc-1 in L-428 cells was validated by qRT-PCR. We also confirm transcriptional downregulation of ERV3 in acute megakaryocytic and erythroid leukemia, and HERVK in acute monocytic and myelocytic leukemia and a depression of HERVF in all malignant entities. Most of the higher expressed HERV families could be detected in stem cells including HERVK (HML-2), HERV-like, HERVV, HERVT, ERV9, HERVW, HERVF, HERVMER, ERV3, HERVH and HERVPABLB.

show abstract

“…HERV-R transcripts have broad expression in normal tissue and due to its overexpression in the first trimester of pregnancy an immunosuppressive function in the mother-fetus interaction was suggested (54,55). ERV3-1 is the only copy of approximately 40 ERV3-like elements in the human genome that has a complete ORF for its ENV (56). Interestingly, the expression of this full-length ENV is associated with several tumor entities including colorectal (57), ovarian (58) and endometrial carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Endogenous Retroviruses and Malignancy Markers in Neuroblastoma Cell Lines by Medium-Induced Microenvironmental Changes

et al. 2021

Self Cite

View full text Add to dashboard Cite

Neuroblastoma (NB) is the commonest solid tumor outside the central nervous system in infancy and childhood with a unique biological heterogeneity. In patients with advanced, metastasizing neuroblastoma, treatment failure and poor prognosis is often marked by resistance to chemo- or immunotherapy. Thus, identification of robust biomarkers seems essential for understanding tumor progression and developing effective therapy. Here, we have studied the expression of human endogenous retroviruses (HERV) as potential targets in NB cell lines during stem-cell medium-induced microenvironmental change. Quantitative PCR revealed that relative expression of the HERV-K family and HERV-W1 ENV were increased in all three NB cell lines after incubation in stem-cell medium. Virus transcriptome analyses revealed the transcriptional activation of three endogenous retrovirus elements: HERV-R ENV (ERV3-1), HERV-E1 and HERV-Fc2 ENV (ERVFC1-1). Known malignancy markers in NB, e.g. proto-oncogenic MYC or MYCN were expressed highly heterogeneously in the three investigated NB cell lines with up-regulation of MYC and MYCN upon medium-induced microenvironmental change. In addition, SiMa cells exclusively showed a phenotype switching from loosely-adherent monolayers to low proliferating grape-like cellular aggregates, which was accompanied by an enhanced CD133 expression. Interestingly, the overexpression of HERV was associated with a significant elevation of immune checkpoint molecule CD200 in both quantitative PCR and RNA-seq analysis suggesting tumor escape mechanism in NB cell lines after incubation in serum-free stem cell medium.

show abstract

Endogenous Retrovirus 3 – History, Physiology, and Pathology

Cited by 20 publications

References 108 publications

Expression of A New Endogenous Retrovirus-Associated Transcript in Hodgkin Lymphoma Cells

Expression of A New Endogenous Retrovirus-Associated Transcript in Hodgkin Lymphoma Cells

Identification of Differentially Expressed Human Endogenous Retrovirus Families in Human Leukemia and Lymphoma Cell Lines and Stem Cells

Overexpression of Endogenous Retroviruses and Malignancy Markers in Neuroblastoma Cell Lines by Medium-Induced Microenvironmental Changes

Contact Info

Product

Resources

About