2004
DOI: 10.1530/rep.1.00173
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Endogenous regulators of protein phosphatase-1 during mouse oocyte development and meiosis

Abstract: Reversible phosphorylation, involving protein kinases and phosphatases (PP), is important in regulating oocyte meiosis. Okadaic acid (OA) inhibition of PP1 and/or PP2A stimulates oocyte germinal vesicle breakdown (GVB). In oocytes, PP1 is localized in the cytoplasm and nucleus, yet endogenous regulation of oocyte PP1 has not been investigated. The objectives of the study were to identify intra-oocyte mechanisms regulating PP1 during acquisition of OA-sensitive meiotic competence and meiotic resumption. Immunoh… Show more

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Cited by 22 publications
(25 citation statements)
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“…This result indicates that CDC25 activity is required in addition to Wee1B downregulation for the meiotic resumption. Because the suppression of CDC25 activity by PKA has been reported, [50][51][52] the low GVBD rate of the pigWee1B-downregulated oocytes might be attributed to the suppression of CDC25 activity by the high cAMP concentration.…”
Section: Discussionmentioning
confidence: 93%
“…This result indicates that CDC25 activity is required in addition to Wee1B downregulation for the meiotic resumption. Because the suppression of CDC25 activity by PKA has been reported, [50][51][52] the low GVBD rate of the pigWee1B-downregulated oocytes might be attributed to the suppression of CDC25 activity by the high cAMP concentration.…”
Section: Discussionmentioning
confidence: 93%
“…An involvement of okadaic acid (OA)-sensitive PP has been demonstrated during oocyte maturation in starfish, Xenopus and mammals (Huchon et al, 1981;Bornslaeger et al, 1986;Goris et al, 1989;Pondaven et al, 1989;Rime and Ozon, 1990;Schwartz and Schultz, 1991;Lu et al, 2001;Wang et al, 2004). Evidence for a crosstalk between cAMP-PKA and OA-sensitive PP was presented as well (Schwartz and Schultz, 1991;Lu et al, 2001).…”
Section: Introductionmentioning
confidence: 97%
“…In metII mouse eggs, OA induces spindle lengthening (26) and has been reported to block meiotic exit (27). However, it is clear that mouse oocytes contain PP1 in addition to PP2A, and some of the effects of OA on prophase I mouse oocytes may be due to PP1 inhibition (28,29).In this study, we decided to further examine the cell cycle effects of OA on mouse metII eggs. We wanted to determine the outcome of OA addition on cyclin B1 and securin degradation specifically because PP2A is needed in Xenopus eggs to hydrolyze CDK1 phosphorylation sites on Emi2, thereby strengthening Emi2 interaction with the APC/C (30 -32).…”
mentioning
confidence: 99%
“…In metII mouse eggs, OA induces spindle lengthening (26) and has been reported to block meiotic exit (27). However, it is clear that mouse oocytes contain PP1 in addition to PP2A, and some of the effects of OA on prophase I mouse oocytes may be due to PP1 inhibition (28,29).…”
mentioning
confidence: 99%