Endogenous prolactin-releasing peptide regulates food intake in rodents

Takayanagi, Yuki; Matsumoto, Hirokazu; Nakata, Masao; Mera, Takashi; Fukusumi, Shoji; Hinuma, Shuji; Ueta, Yoichi; Yada, Toshihiko; Leng, Gareth; Onaka, Tatsushi

doi:10.1172/jci34682

Cited by 81 publications

(120 citation statements)

References 45 publications

(80 reference statements)

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…UCP1 mRNA levels were determined using the Threshold Cycle method in accordance with the manufacturer's protocol. To standardize the level of UCP1 mRNA, glyceraldehyde-3-phosphate dehydrogenase (Gapd) mRNA, one of the housekeeping genes, was used for the reference in the same way as in a previous study in which the UCP1 mRNA level during cold exposure was assessed [18].…”

Section: Methodsmentioning

confidence: 99%

Estrogen modulates central and peripheral responses to cold in female rats

et al. 2009

View full text Add to dashboard Cite

The aim of this study was to determine whether estrogen modulates central and peripheral responses to cold in female rats. In ovariectomized female rats with and without administered estrogen [E(2) (+) and E(2) (-), respectively], the counts of cFos-immunoreactive cells in the medial preoptic nucleus (MPO) and dorsomedial hypothalamic nucleus (DMH) in the hypothalamus were greater in the E(2) (+) rats than in the E(2) (-) rats at 5 degrees C. Examination of the response of normal female rats to exposure to 5 degrees C at different phases of the estrus cycle revealed that counts of cFos-immunoreactive cells in the MPO, DMH, and posterior hypothalamus and the level of uncoupling protein 1 mRNA in the brown adipose tissues were greater in the proestrus phase than on day 1 of the diestrus phase. This result was linked to the level of plasma estrogen. The body temperature during cold exposure was higher in the E(2) (+) rats than in the E(2) (-) rats and was also higher in the proestrus phase than on day 1 of the diestrus phase. We conclude that estrogen may affect central and peripheral responses involved in thermoregulation in the cold.

show abstract

Section: Methodsmentioning

confidence: 99%

Estrogen modulates central and peripheral responses to cold in female rats

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Recently, it has been established that PrRP has other physiological functions (Onaka et al 2010), including the regulation of food intake (Lawrence et al 2000) and energy expenditure (Takayanagi et al 2008), whereas its involvement in the regulation of hypothalamic-pituitary-adrenal (HPA) axis (Dodd & Luckman 2013) and its prolactin-releasing ability was questioned (Jarry et al 2000). PrRP-producing cells are localized in the dorsomedial hypothalamic nucleus and in A1/A2 regions of the medulla oblongata (Yamada et al 2009) in the brainstem.…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown in rodents that intracerebroventricular injection of PrRP decreased food intake and body weight . Moreover, mice deficient in Prrp or Prrp receptor are obese (Gu et al 2004, Takayanagi et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Palmitoylated PrRP analog decreases body weight in DIO rats but not in ZDF rats

Holubová

Zemenová

Mikulášková

et al. 2016

Journal of Endocrinology

View full text Add to dashboard Cite

Anorexigenic neuropeptides produced and acting in the brain have the potential to decrease food intake and ameliorate obesity, but are ineffective after peripheral application, owing to a limited ability to cross the blood–brain barrier. We have designed lipidized analogs of prolactin-releasing peptide (PrRP), which is involved in energy balance regulation as demonstrated by obesity phenotypes of bothPrrp-knockout andPrrpreceptor-knockout mice. The aim of this study was to characterize the subchronic effect of a palmitoylated PrRP analog in two rat models of obesity and diabetes: diet-induced obese Sprague–Dawley rats and leptin receptor-deficient Zucker diabetic (ZDF) rats. In the rats with diet-induced obesity (DIO), a two-week intraperitoneal treatment with palmitoylated PrRP lowered food intake by 24% and body weight by 8%. This treatment also improved glucose tolerance and tended to decrease leptin levels and adipose tissue masses in a dose-dependent manner. In contrast, in ZDF rats, the same treatment with palmitoylated PrRP lowered food intake but did not significantly affect body weight or glucose tolerance, probably in consequence of severe leptin resistance due to a nonfunctional leptin receptor. Our data indicate a good efficacy of lipidized PrRP in DIO rats. Thus, the strong anorexigenic, body weight-reducing, and glucose tolerance-improving effects make palmitoylated PrRP an attractive candidate for anti-obesity treatment.

show abstract

“…Shortly after its discovery, it was established that PrRP has other physiological functions, including the regulation of food intake (Lawrence et al 2000) and energy expenditure (Takayanagi et al 2008). PrRP and its receptor were detected in several hypothalamic nuclei as well as in brainstem suggesting an involvement of PrRP in the control of food intake and body weight regulation (Roland et al 1999, Ibata et al 2000.…”

Section: Prolactin-releasing Peptide In Food Intake Regulationmentioning

confidence: 99%

“…Similarly, PrRP-deficient mice displayed late-onset obesity, increased food intake and attenuated responses to the anorexigenic signals cholecystokinin and leptin (Takayanagi et al 2008).…”

Section: Prolactin-releasing Peptide In Food Intake Regulationmentioning

confidence: 99%

Prolactin-releasing peptide: a new tool for obesity treatment

Kuneš

Pražienková

Popelová

et al. 2016

Journal of Endocrinology

View full text Add to dashboard Cite

Obesity is an escalating epidemic, but an effective noninvasive therapy is still scarce. For obesity treatment, anorexigenic neuropeptides are promising tools, but their delivery from the periphery to the brain is complicated because peptides have a low stability and limited ability to cross the blood-brain barrier. In this review, we summarize results of several studies with our newly designed lipidized analogs of prolactin-releasing peptide (PrRP). PrRP is involved in feeding and energy balance regulation as demonstrated by obesity phenotypes of both PrRP-and PrRP-receptor-knockout mice. Lipidized PrRP analogs showed binding affinity and signaling in PrRP receptor-expressing cells similar to natural PrRP. Moreover, these analogs showed high binding affinity also to anorexigenic neuropeptide FF (NPFF)-2 receptor. Acute peripheral administration of myristoylated and palmitoylated PrRP analogs to mice and rats induced strong and long-lasting anorexigenic effects and neuronal activation in the brain areas involved in food intake regulation. Two-week-long subcutaneous administration of palmitoylated PrRP31 and myristoylated PrRP20 lowered food intake, body weight, improved metabolic parameters and attenuated lipogenesis in mice with diet-induced obesity. A strong anorexigenic, body weight-reducing and glucose tolerance-improving effect of palmitoylated-PrRP31 was shown also in diet-induced obese rats after its repeated 2-week-long peripheral administration. Thus, the strong anorexigenic and body weight-reducing effects of palmitoylated PrRP31 and myristoylated PrRP20 make these analogs attractive candidates for antiobesity treatment. Moreover, PrRP receptor might be a new target for obesity therapy.

show abstract

Endogenous prolactin-releasing peptide regulates food intake in rodents

Cited by 81 publications

References 45 publications

Estrogen modulates central and peripheral responses to cold in female rats

Estrogen modulates central and peripheral responses to cold in female rats

Palmitoylated PrRP analog decreases body weight in DIO rats but not in ZDF rats

Prolactin-releasing peptide: a new tool for obesity treatment

Contact Info

Product

Resources

About