Endogenous production of tumor necrosis factor by a combination of interferon and BRM of bacterial origin

Effects of Liposome-Entrapped Muramyl Tripeptide Phosphatidylethanolamine (L-MTP-PE) on the Tumor Growth and Survival of Mice Bearing Syngeneic Tumor in Combination with a Chemotherapeutic or Immunomodulatory Agent

“…Briefly, at 3 h after a priming agent was injected to C3H/He mice, a triggering agent, OK432 12 were administered intravenously, then at 2 h later, their blood was withdrawn under anesthesia. TNF-αactivities of separated serum were measured according to the method of Satoh et al 13 by using an in vitro cytotoxicity assay with L-929 cells as targets by the method of Ruff et al 14 value for the dilution of cytotoxic serum for 50% survival of the cells (ED 50 ) was obtained from a dose response curve. TNF-α activity (units/mL) was calculated from the ratio of the ED 50 of the test serum to that of standard rabbit tumor necrosis serum, the titer of which was determined to be 6,000 units/mL of recombinant human TNF-α (1 x 105 units/mL, kindly supplied by Asahi Chemical Co. LTD., Osaka, Japan) in each test plate.…”

“…C3H/He mice, BALB/c mice and C57B1/6 mice were used for the inoculations of MM46 carcinoma (MM46), Meth A fibrosarcoma (Meth A), and M5076 carcinoma (M5076), respectively. MM46 and Meth A were maintained in our laboratory as described previously 13 14 . M5075 were kindly donated by Drs.…”

Inhibition by Perilla Juice of Tumor Necrosis Factor Production

“…Serum TNF activity was measured by an in vitro cytotoxicity assay with L-929 cells as a target. 3) Units of activity were calculated as the dilution factor of the serum allowing survival of half the L-929 cells with a human recombinant TNF preparation as a standard.…”