Endogenous phytonutrient, phytochemical, and phytohormone levels modulate <i>in-vitro</i> callus induction and plant regeneration in finger millet (<i>Eleusine coracana</i>) genotypes

Jamra, Gautam; Shah, Pallavi; Agarwal, Aparna; Sharma, Divya; Kumar, Anil

doi:10.1080/11263504.2021.1918779

Cited by 5 publications

(1 citation statement)

References 52 publications

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“…Nevertheless, in this study, two types of shoots were obtained from the stem nodes of Jerusalem artichoke cultured on the same medium containing 0.5 mg/L 6-BA. The influences of the types, concentrations, and combinations of endogenous hormones on callus induction and callus morphogenesis have been documented in various plants [49][50][51]. Endogenous IAA is a crucial factor determining callus induction [51,52], whereas endogenous ABA is usually considered an inhibitor and is involved in various environmental stress responses [23,53].…”

Section: Differences In the Endogenous Hormone Content And Antioxidan...mentioning

confidence: 99%

Plant Regeneration via Organogenesis in Jerusalem Artichokes and Comparative Analysis of Endogenous Hormones and Antioxidant Enzymes in Typical and Atypical Shoots

Zhang,

Yin

et al. 2023

Plants

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The Jerusalem artichoke (Helianthus tuberosus) is a tuberous plant with considerable nutrient and bioactive compounds. The optimization of the in vitro clonal propagation protocol is critical for large-scale reproduction and biotechnological applications of Jerusalem artichoke production. In this work, in vitro plant regeneration from the stem nodes of the Jerusalem artichoke via direct organogenesis is presented. In the shoot induction stage, the stem segments produced more shoots with vigorous growth on MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA). The concentrations of 6-BA and gibberellic acid (GA3) were both optimized at 0.5 mg/L for shoot multiplication, and the combination of 0.05 mg/L indole-3-butyric acid (IBA) and 0.05 mg/L 1-naphthylacetic acid (NAA) was the most responsive for root induction, yielding the largest number of roots. The regenerated plantlets were successfully hardened at a 96% survival rate and vigorously grew in the field. The genetic stability of the regenerated plants was confirmed by flow cytometry and simple sequence repeat (SSR) analysis. However, 17.3% of shoots on the optimum shoot induction medium had withered leaves and excessive callus (atypical shoots), which greatly reduced the induction efficiency. Enzyme activity in the typical and atypical shoots was compared. The atypical shoots had significantly higher levels of endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA), as well as increased activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), whereas the content of 6-BA, zeatin (ZT), and GA3 was significantly reduced. The activity of the three enzymes was positively correlated with the content of IAA and ABA, while being negatively correlated with that of 6-BA, ZT, and GA3. The results suggest that the poor growth of the atypical shoots might be closely related to the significant accumulation of endogenous IAA and ABA, thus significantly increasing antioxidant enzyme activity.

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