Endogenous Phytohormones of Fern Polystichum aculeatum (L.) Roth Gametophytes at Different Stages of Morphogenesis in vitro Culture

Kosakivska, I.V.; Vasyuk, V.A.; Voytenko, L.V.; Shcherbatiuk, М. М.; Romanenko, K. O.; Бабенко, Л. М.

doi:10.3103/s0095452720010089

Cited by 14 publications

(11 citation statements)

References 49 publications

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“…The combined supernatants were evaporated to 5 mL with a Typ 350P vacuum evaporator (Poland). Further purification of phytohormones was performed by the method (Kosakivska et al, 2020) on two solid phase SPE C18 columns, Sep-Pak Plus, Waters and SPE Oasis MCX, 6 cc / 150 mg, Waters. Column C18 was used to remove lipophilic substances, proteins and pigments.…”

Section: Methodsmentioning

confidence: 99%

Morphogenesis, pigment content, phytohormones and productivity of eggplants under the action of gibberellin and tebuconazole

Rogach

Voytenko

Shcherbatiuk

et al. 2020

Regul. Mech. Biosyst.

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Regulation of growth and development of cultivated plants in order to optimize the production process is one of the main objectives of modern plant physiology. Both activators and growth inhibitors are used for this purpose. The effect of foliar treatment with 0.005% solution of gibberellic acid and 0.025% solution of antigibberellic preparation of tebuconazole on the morphogenesis, leaf structure, photosynthetic pigment content, balance of phytohormones and eggplant productivity cv. Diamond variety was investigated. The treatment with the preparations was carried out in the budding phase. The vegetation experiment was laid under soil and sand culture in vessels of a ten-liter capacity. The morphometric parameters were determined every 10 days, the mesostructure was studied in the leaves of the middle tier in the phase of fruit formation, chlorophyll content was measured in the raw material by spectrophotometric method. Analytical determination of phytohormones (indole-3-acetic acid, gibberellic, abscisic acids and cytokinins – zeatin, zeatin-O-glucoside, zeatinriboside, isopentenyladenine and isopentenyladenosine) was performed by high performance liquid chromatography. Under the action of gibberellic acid, plant height increased significantly, and it decreased after its treatment with tebuconazole. The stimulator and inhibitor increased the number of leaves per plant, the weight of the leaf dry matter, the area of the individual leaf blade and the area of leaves per plant. Both preparations increased the weight of the raw material of the stems and roots, as well as the mass of dry matter of the whole plant. Under the action of tebuconazole, the content of chlorophylls in the leaves increased, while under the action of gibberellic acid it decreased. After treatment with gibberellic acid and tebuconazole, the thickness of the leaf blade increased due to chlorenchyma thickening. In the variant with gibberellic acid, the thickness of upper and lower epidermis increased, and in the variant with tebuconazole, these parameters decreased. Both growth regulators increased the volume of cells of the columnar parenchyma. The contet of endogenous gibberellic, indole-3-acetic and abscisic acids of the stems and especially in the leaves increased with the treatment of exogenous gibberellic acid, whereas after the application of tebuconazole the gibberellic and indole-3-acetic acid content of the stems decreased significantly and practically decreased to traces. Instead, the amount of abscisic acid increased. After treatment with exogenous gibberellic acid, the pool of cytokinins in the leaves significantly decreased. The inactive isoforms of the hormone zeatin-O-glucoside and isopentenyladenosine dominated in the stems. The effect of tebuconazole decreased the pool of cytokinins in the stems and increased in the leaves. In general, due to the multidirectional action, growth regulators positively influenced the elements of eggplant productivity. More effective was the retardant – tebuconazole. Therefore, the anatomic-morphological and structural-functional rearrangements in eggplants under the effects of exogenous gibberellic acid and tebuconazole are due to changes in the balance and distribution of endogenous hormones. Increased photosynthetic activity, stimulation of growth processes of some organs of the plant and inhibition of others enlarged the biological productivity of the culture.

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Section: Methodsmentioning

confidence: 99%

Morphogenesis, pigment content, phytohormones and productivity of eggplants under the action of gibberellin and tebuconazole

Rogach

Voytenko

Shcherbatiuk

et al. 2020

Regul. Mech. Biosyst.

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“…Unfortunately, understanding of the biochemistry and metabolism of Cannabis beyond the cannabinoid pathways is limited and represents a necessary area of future study. To date, several studies [ 120 , 121 , 122 , 123 , 124 ] showed that the gene expression pattern of endogenous PGRs and the balance between endogenous and exogenous PGRs play an important role in regeneration efficiency, especially in recalcitrant plants. For instance, Kumari et al [ 120 ] studied the endogenous level of PGRs via UHPLC–MS analysis and its effect on shoot regeneration and somatic embryogenesis of Tulbaghia simmleri .…”

Section: Future Directionsmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

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Hesami

et al. 2021

Plants

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

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“…Notably, the endogenous IAA content displayed a similar trend to that of ABA. Kosakivska et al [ 20 ] reported a similar balance between the endogenous ABA and IAA contents analyzed from Polystichum aculeatum gametophytes of the same morphogenesis stage. Although auxin is involved in the growth of gametophytes, it accumulates primarily in young sporophytes and promotes development [ 32 ].…”

Section: Discussionmentioning

confidence: 76%

“…Zeatin, dihydrozeatin, zeatin-riboside, and isopentenyladenine were detected at similar levels in both gametophytes and sporophytes [ 19 ]. Kosakivska et al [ 20 ] reported zeatin glucoside and isopentenyladenin in both the SG and sporophyte stages. In this study, zeatin was detected in the control, GS, and GL.…”

Section: Discussionmentioning

confidence: 99%

Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

et al. 2021

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Background Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension culture method. Results The growth curve linear phase of gametophyte cells was confirmed between 9 and 18 days of culture, and the subculture cycle was determined to be 2 weeks. A double-strength MS medium (fresh weight, 18.0 g) containing 2% sucrose and NH4+:NO3− (120 mM, 40:80) was found to be the optimal liquid medium. Gametophytes obtained after suspension culture for 18 days did not normally form sporophytes in an ex vitro soil environment. However, this issue was resolved after changing the culture type or extending the culture period to 6 weeks. A short suspension culture period increased the fresh weight of fragmented and homogenized gametophytes but yielded numerous relatively immature gametophytes (globular forms of branching gametophytes, BG). Furthermore, differences in gametophyte morphogenesis and development were indicated by changes in endogenous phytohormone content. BG with immature development exhibited high accumulation of zeatin, jasmonic acid, and salicylic acid, and relatively low levels of abscisic acid and indole-3-acetic acid. The immature development of gametophytes directly affected sporophyte formation. Conclusions This study maximized the advantages of liquid suspension culture using eastern bracken gametophytes and provides data to resolve any associated issues, thus facilitating efficient bracken production.

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Endogenous Phytohormones of Fern Polystichum aculeatum (L.) Roth Gametophytes at Different Stages of Morphogenesis in vitro Culture

Cited by 14 publications

References 49 publications

Morphogenesis, pigment content, phytohormones and productivity of eggplants under the action of gibberellin and tebuconazole

Morphogenesis, pigment content, phytohormones and productivity of eggplants under the action of gibberellin and tebuconazole

The Past, Present and Future of Cannabis sativa Tissue Culture

Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

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